印度北部和东部地区乙型肝炎病毒基因型和亚基因型的流行:DNA测序方法

Q3 Pharmacology, Toxicology and Pharmaceutics
J. Kandpal, Rishendra Kumar, Kailash Chandra, S. Pandey, T. S. Bisht
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引用次数: 0

摘要

印度乙型肝炎病毒基因型和亚基因型的患病率摘要简介:乙型肝炎病毒(HBV)是一种在肝脏相关疾病中高度流行的感染病毒。HBV基因型和亚基因型的遗传分布和鉴定是控制感染传播的一个挑战。为了了解分子流行率,本方法对印度北部和东部地区HBV基因型和亚基因型的分布模式进行了研究。方法:对67例HBV DNA阳性受试者进行研究。首先,通过实时PCR对HBV阳性病例的DNA样本进行筛选,然后扩增HBV聚合酶基因的选定区域进行序列分析,以确定基因型和亚基因型。结果:常见基因型为D型(62.68%),其次为A型(29.85%)和C型(7.46%)。C1亚型仅在东部地区发现。A1亚型的频率在北部地区较高(n=13,30.95%),其次是东部地区(n=7,28%)。在15名(35.71%)受试者中发现HBV亚型D1占主导地位,其次是来自北部地区的10名(23.80%)受试人中的亚型D2。在来自东部地区的8名(32%)受试者中发现HBV亚型D2占优势。结论:总之,该方法清楚地表明了该地区D1、D2、A1亚型的高患病率。此外,在东部地区对C1亚基因型的鉴定强调了高传播感染风险和向印度其他地区的传播途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Prevalence of hepatitis B virus genotypes and sub-genotypes in north and east regions of India: DNA sequencing methodology
Prevalence of hepatitis B virus genotypes and sub-genotypes in India Abstract Introduction: Hepatitis B virus (HBV) is a highly prevalent infecting virus among liver-related diseases. The genetic distribution and identification of HBV genotypes and sub-genotypes represented a challenge to control the spread of infection. To find out molecular prevalence, the present methodology was carried out for the distribution pattern of HBV genotypes and subgenotypes in north and east regions of India. Methods:A total of 67 HBV DNA positive subjects were studied. At first, the DNA samples for HBV positive cases were screened by Real-time PCR, and then the selected region of HBV polymerase gene was amplified for the sequence analysis to determine genotypes and sub-genotypes. Results:The prevalent genotype found was the genotype D (62.68 %), followed by genotype A (29.85 %), and genotype C (7.46 %). Subgenotype C1 was identified in the east region only. The frequency of sub-genotype A1 was higher in the north region (n = 13, 30.95 %) followed by east region (n = 7, 28 %). HBV sub-genotype D1 was found to be predominant in 15 (35.71 %) subjects followed by sub-genotypes D2 in 10 (23.80 %) subjects from the north region. HBV sub-genotype D2 was found to be predominant in 8 (32 %) subjects from the east region. Conclusions:In conclusion, the method clearly demonstrates the high prevalence of subgenotypes D1, D2, A1 in this region. Also, the identification of the sub-genotype C1 in the east region emphasizes the high transmission infection risk and transmission route towards other regions of India.
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来源期刊
Current Trends in Biotechnology and Pharmacy
Current Trends in Biotechnology and Pharmacy Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
0.70
自引率
0.00%
发文量
0
期刊介绍: The Association of Biotechnology and Pharmacy (ABAP) will be useful to form a forum for scientists so that they can bring together to discuss and find scientific solutions to the problems of society. The annual meetings will help the members to share their knowledge and publish their research knowledge particularly by members and fellows of the Association and special care will be taken to provide an opportunity for young scientists. Besides this the association is planned to organize symposia, seminars and workshops on current developments of Biotechnology and Pharmacy particularly on the subject of current scientific interest, and the proceedings of which will be published regularly. And in view of the vast development of science and to disseminate the problems in publication of research work, an international journal of Current Trends in Biotechnology and Pharmacy has been started by ABAP.
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