Anodal阻滞评价麻醉大鼠神经传导变化的临床前非随机实验研究

V. Pokrovskiy, A. N. Ardelyan, B. S. Tashu, N. A. Arutyunyan, O. Y. Sherbakov, S. S. Pilipenko, D. A. Pocheshkhova
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A DC anode was placed on the exposed sciatic nerve, more proximal and more distal to the recording electrodes, and a common cathode in the form of a needle was introduced into one of the forelimbs. Needle nichrome electrodes were introduced into the nerve using a manipulator. An original neurogram and a neurogram against anode activation were recorded by closing the DC circuit of different voltages to block the afferent signal, efferent signal and afferent and efferent signals simultaneously.Results. When the anodal block of different voltages was applied to the afferent signal, efferent signal, and afferent and efferent signals simultaneously in all 10 experiments, the frequency-amplitude characteristics of the neurogram changed significantly as compared to the original neurogram. The amplitude of the neurogram increased considerably, while the frequency decreased, though not so dramatically. 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引用次数: 0

摘要

背景目前还没有神经再生功能评估的黄金标准。不同的研究人员使用各种方法来直接和间接评估再生神经的功能。间接方法的优点是微创,直接方法的好处是直接在神经中记录信号。目标。确定应用阳极块时麻醉大鼠坐骨神经的神经图变化的重要参数,并评估神经造影作为神经再生功能评估的方法。方法。在10只麻醉大鼠身上进行了一系列实验。将直流阳极放置在暴露的坐骨神经上,更靠近和更远离记录电极,并将针形式的公共阴极引入其中一个前肢。使用机械手将针状镍铬电极引入神经。通过闭合不同电压的直流电路来同时阻断传入信号、传出信号以及传入和传出信号,记录原始神经图和反对阳极激活的神经图。后果在所有10个实验中,当对传入信号、传出信号以及传入和传出信号同时施加不同电压的阳极阻断时,与原始神经图相比,神经图的频率-振幅特性发生了显著变化。神经图的振幅显著增加,而频率降低,尽管没有那么显著。振幅和频率参数的变化取决于电压值。在大多数情况下,这种关系与振幅成正比,与频率成反比。结论考虑到暴露于阳极阻断时神经图的动力学性质,其变化的最重要参数是振幅。损伤后再生过程中神经纤维成分的变化会导致传入和传出信号的变化,与初始状态相比,这可能会在神经图中显示出来。因此,阳极块可以用作神经损伤的模型,以及神经图参数的动力学分析,作为神经再生功能评估的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Anodal Block in Evaluation of Nerve Conduction Changes in Anesthetized Rats: Preclinical Non-Randomized Experimental Study
Background. There is currently no gold standard for functional assessment of nerve regeneration. Different researchers use various methods to assess the functionality of the regenerated nerve directly and indirectly. Indirect methods have the advantage of being minimally invasive, and the benefit of direct methods is recording the signal directly in the nerve.Objectives. To identify significant parameters of neurogram changes in the sciatic nerve in an anaesthetized rat when the anode block is applied and to evaluate neurography as a method for functional assessment of nerve regeneration.Methods. A series of experiments was performed on 10 anaesthetized rats. A DC anode was placed on the exposed sciatic nerve, more proximal and more distal to the recording electrodes, and a common cathode in the form of a needle was introduced into one of the forelimbs. Needle nichrome electrodes were introduced into the nerve using a manipulator. An original neurogram and a neurogram against anode activation were recorded by closing the DC circuit of different voltages to block the afferent signal, efferent signal and afferent and efferent signals simultaneously.Results. When the anodal block of different voltages was applied to the afferent signal, efferent signal, and afferent and efferent signals simultaneously in all 10 experiments, the frequency-amplitude characteristics of the neurogram changed significantly as compared to the original neurogram. The amplitude of the neurogram increased considerably, while the frequency decreased, though not so dramatically. The changes in amplitude and frequency parameters were revealed to depend on the voltage value. In most cases, this relationship was directly proportional to the amplitude and inversely proportional to the frequency.Conclusion. Considering the nature of the dynamics of the neurogram when exposed to the anodal block, the most significant parameter of its change is the amplitude. Changes in nerve fibre composition during its regeneration after damage cause changes in afferent and efferent signals, which is likely to be displayed in the neurogram as compared to the initial state. Thus, the anodal block can be used as a model of nerve damage, and the analysis of the dynamics of neurogram parameters — as a method for functional assessment of nerve regeneration.
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CiteScore
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