Windy E. Cole, Janina A. Krumbeck, Christopher Schattinger
{"title":"一项单中心试点研究调查了天然交联细胞外基质与聚六亚甲基双胍治疗慢性下肢伤口细菌污染的效果。","authors":"Windy E. Cole, Janina A. Krumbeck, Christopher Schattinger","doi":"10.25270/wmp.2023.2.22077","DOIUrl":null,"url":null,"abstract":"BACKGROUND\nElevated bacterial bioburden and biofilm formation are common causes for delayed wound healing. Biofilm is an assemblage of surface-associated microbes enclosed in a self-produced matrix. Identifying and managing biofilm is a crucial component of successful wound care protocols.\n\n\nPURPOSE\nTo determine the effects of a native cross-linked extra cellular matrix with polyhexamethylene biguanide (PHMP; PHMP antimicrobial [PCMP]), in conjunction with next-generation DNA sequencing (NGS) and targeted debridement by fluorescence imaging, on managing bacterial bioburden to support wound healing.\n\n\nMETHODS\nOver 6 weeks, fluorescence images were captured to assess bacterial contamination as were standard images with measurements. Tissue swabs were obtained and analyzed using NGS to quantify bacterial bioburden. PCMP was applied at each treatment visit.\n\n\nRESULTS\nBaseline wound duration was 23.0 ± 6.7 weeks, and baseline size was 7.9 ± 7.1 cm2. Two out of 5 wounds healed by week 4. The mean percentage area reduction of all wounds was 59.7% by week 4 and 78.1% by week 6. All wounds were fluorescence positive on week 1 and fluorescence negative by Week 4.\n\n\nCONCLUSION\nPCMP, as an antimicrobial barrier, combined with use of a real-time method of bacterial detection, may be beneficial in managing the wound healing environment.","PeriodicalId":23741,"journal":{"name":"Wound management & prevention","volume":null,"pages":null},"PeriodicalIF":1.0000,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A single-center pilot study investigating the effects of a native cross-linked extracellular matrix with polyhexamethylene biguanide to manage chronic lower extremity wounds exhibiting bacterial contamination.\",\"authors\":\"Windy E. Cole, Janina A. Krumbeck, Christopher Schattinger\",\"doi\":\"10.25270/wmp.2023.2.22077\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"BACKGROUND\\nElevated bacterial bioburden and biofilm formation are common causes for delayed wound healing. Biofilm is an assemblage of surface-associated microbes enclosed in a self-produced matrix. Identifying and managing biofilm is a crucial component of successful wound care protocols.\\n\\n\\nPURPOSE\\nTo determine the effects of a native cross-linked extra cellular matrix with polyhexamethylene biguanide (PHMP; PHMP antimicrobial [PCMP]), in conjunction with next-generation DNA sequencing (NGS) and targeted debridement by fluorescence imaging, on managing bacterial bioburden to support wound healing.\\n\\n\\nMETHODS\\nOver 6 weeks, fluorescence images were captured to assess bacterial contamination as were standard images with measurements. Tissue swabs were obtained and analyzed using NGS to quantify bacterial bioburden. PCMP was applied at each treatment visit.\\n\\n\\nRESULTS\\nBaseline wound duration was 23.0 ± 6.7 weeks, and baseline size was 7.9 ± 7.1 cm2. Two out of 5 wounds healed by week 4. The mean percentage area reduction of all wounds was 59.7% by week 4 and 78.1% by week 6. All wounds were fluorescence positive on week 1 and fluorescence negative by Week 4.\\n\\n\\nCONCLUSION\\nPCMP, as an antimicrobial barrier, combined with use of a real-time method of bacterial detection, may be beneficial in managing the wound healing environment.\",\"PeriodicalId\":23741,\"journal\":{\"name\":\"Wound management & prevention\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2023-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Wound management & prevention\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.25270/wmp.2023.2.22077\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"DERMATOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Wound management & prevention","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.25270/wmp.2023.2.22077","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"DERMATOLOGY","Score":null,"Total":0}
A single-center pilot study investigating the effects of a native cross-linked extracellular matrix with polyhexamethylene biguanide to manage chronic lower extremity wounds exhibiting bacterial contamination.
BACKGROUND
Elevated bacterial bioburden and biofilm formation are common causes for delayed wound healing. Biofilm is an assemblage of surface-associated microbes enclosed in a self-produced matrix. Identifying and managing biofilm is a crucial component of successful wound care protocols.
PURPOSE
To determine the effects of a native cross-linked extra cellular matrix with polyhexamethylene biguanide (PHMP; PHMP antimicrobial [PCMP]), in conjunction with next-generation DNA sequencing (NGS) and targeted debridement by fluorescence imaging, on managing bacterial bioburden to support wound healing.
METHODS
Over 6 weeks, fluorescence images were captured to assess bacterial contamination as were standard images with measurements. Tissue swabs were obtained and analyzed using NGS to quantify bacterial bioburden. PCMP was applied at each treatment visit.
RESULTS
Baseline wound duration was 23.0 ± 6.7 weeks, and baseline size was 7.9 ± 7.1 cm2. Two out of 5 wounds healed by week 4. The mean percentage area reduction of all wounds was 59.7% by week 4 and 78.1% by week 6. All wounds were fluorescence positive on week 1 and fluorescence negative by Week 4.
CONCLUSION
PCMP, as an antimicrobial barrier, combined with use of a real-time method of bacterial detection, may be beneficial in managing the wound healing environment.