乌克兰栽培的小白菊(Tanacetum Parthenium)中Parthenolide的定性组成和定量含量研究

European Pharmaceutical Journal Pub Date : 2020-05-01 DOI:10.2478/afpuc-2020-0011

K. Hordiei, T. Gontova, S. Gubar, M. Yaremenko, E. Kotova

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引用次数: 0

摘要

摘要小白菊是一种著名的药用植物，是倍半萜内酯的重要来源，其主要成分是一种孤雌醇内酯(约占85%)。小白菊因其所含的帕thenolide，具有抗炎作用，被广泛用于预防偏头痛。目的是研究乌克兰栽培的小白菊样品中帕thenolide的定性组成和定量含量，并研究环境因素对帕thenolide积累的影响。方法采用薄层色谱法，在默克公司生产的硅胶60f254板上对小白菊样品进行鉴别。采用ProStar高效液相色谱仪和二极管-矩阵检测器对其进行定量分析。结果采用薄层色谱法对小白菊样品的色谱图谱进行了研究。在所有样品的测试溶液的色谱图中，在parthenolide水平上检测到蓝色区域。其含量范围为0.16% ~ 0.39%。在乌克兰中部地区的样品中观察到的含量最高。结果表明:黑土、日晒量不高于5.25 kWh/m2/day、平均气温+20℃~ +25℃是小白菊类草本植物单酚内酯积累的最佳条件。计算得到的验证参数均满足必要的验收标准。结论对乌克兰不同地区种植的小白菊样品中孤雌酚内酯的定性组成和定量含量进行了研究。根据Eur.Ph.。和SPhU，在所有样品中鉴定出一种孤香内酯。采用高效液相色谱法测定所研究样品中孤香内酯的定量含量。确定了小白菊中孤雌醇内酯积累的最佳条件。

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Study of the Qualitative Composition and Quantitative Content of Parthenolide in the Feverfew (Tanacetum Parthenium) Herb Cultivated in Ukraine

Abstract Feverfew is a well-known medicinal plant as a source of the sesquiterpene lactones, the main one is a parthenolide (about 85%). Owing to the parthenolide, feverfew herb has anti-inflammatory activity and it is widely used for prevention of migraine. The aim was to study the qualitative composition and quantitative content of the parthenolide in the samples of the feverfew herb cultivated in Ukraine and to study the influence of environmental factors on the accumulation of parthenolide. Methods The identification in the samples of the feverfew herb by the thin-layer chromatography method was carried out on silica gel 60 F254 plates, Merck. For the quantitative determination of the parthenolide, a high-performance liquid chromatography ProStar equipped with a diode-matrix detector, were used. Results The chromatographic profile of the samples of the feverfew herb was studied using a TLC method. In the chromatograms of test solutions from all samples, the blue zones were detected at the level of parthenolide. The quantitative content of parthenolide ranged between 0.16% and 0.39%. The highest content was observed in the sample from the Central region of Ukraine. The data obtained indicate that the optimal conditions for parthenolide accumulation in feverfew herb are as follows: black soils, solar insolation not higher than 5.25 kWh/m2/day, average air temperature from +20°C to +25°C. All the calculated validation parameters of the parthenolide quantitative determination method meet the necessary acceptance criteria. Conclusion The study of the qualitative composition and quantitative content of parthenolide in the samples of feverfew herb cultivated in different regions of Ukraine has been conducted. According to the methodology of Eur.Ph. and SPhU, a parthenolide was identified in all the samples. HPLC method was used to determine the quantitative content of parthenolide in the samples studied. The optimal conditions for parthenolide accumulation in feverfew herb were established.

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来源期刊

European Pharmaceutical Journal Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)

CiteScore

0.60

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0.00%

发文量

期刊介绍： European Pharmaceutical Journal publishes only original articles not previously published and articles that are not being considered or have not been submitted for publication elsewhere. If parts of the results have been published as conference abstract or elsewhere, it should be stated in references. The ethical standards of the Helsinki-Tokio Declaration should be kept. This should be mentioned in the Methods of manuscript. Reviews are published only on request. Authors, whose submitted research work was performed with the support of a company, should indicate this in Conflict of Interest.