来自珍珠斑的组蛋白H2A氨基末端衍生肽Etroplusin，Etroplus suratensis（Bloch，1790）：系统发育和功能表征

Q4 Biochemistry, Genetics and Molecular Biology

Animal Gene Pub Date : 2023-06-01 DOI:10.1016/j.angen.2023.200149

P.P. Athira, M.V. Anju, V.V. Anooja, K. Archana, Dhanya Kesavan, S. Neelima, Anjali S. Mohan, M.R. Revathy, Rosamma Philip

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引用次数: 0

摘要

源自组蛋白前体的抗菌肽（AMP）形成了一类重要的治疗分子。本研究探索了核心组蛋白H2A衍生的抗菌肽——珍珠斑Etroplusin、苏拉氏Etroplus suratensis的综合分子特征。Etroplusin表现出AMP的特征性特征，如净+11电荷和两亲性α-螺旋结构。多序列比对显示了etroplusin与先前报道的H2A肽的同源性。系统发育分析表明，etroplusin与鲫鱼H2A衍生肽相似，属于鱼类H2A肽分支。生物信息学工具的结构建模预测了α螺旋结构和脯氨酸铰链的随机线圈。etroplusin的功能表征揭示了其抗菌、抗真菌和抗癌活性。在用细菌性鱼类病原体无乳链球菌攻击时，在苏拉氏杆菌的全身组织中观察到etroplusin的显著上调。本研究将阐明鱼类的先天免疫反应，以及作为防御分子的H2A衍生肽etroplusin的重要性。

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A histone H2A amino terminal derived peptide Etroplusin from Pearl spot, Etroplus suratensis (Bloch, 1790): Phylogenetic and functional characterization

Antimicrobial peptides (AMPs) derived from histone precursors form a significant class of therapeutic molecules. The study explores the comprehensive molecular characterization of core histone H2A-derived antimicrobial peptide, Etroplusin from Pearl spot, Etroplus suratensis. Etroplusin exhibited characteristic features of AMP such as net +11 charge and amphipathic alpha-helical structure. Multiple sequence alignment showed the homologous nature of etroplusin with previously reported H2A peptides. Phylogenetic analysis revealed that etroplusin is similar to Carassius auratus H2A-derived peptide and comes under the fish H2A peptide clade. Structure modelling with bioinformatics tools predicted alpha-helical structure and random coils with a proline hinge. The functional characterization of etroplusin revealed its antibacterial, antifungal, and anticancer activities. Significant upregulation of etroplusin was observed in the systemic tissues of E. suratensis on a challenge with the bacterial fish pathogen, Streptococcus agalactiae. The present study will shed light on fish innate immune responses and the importance of etroplusin, the H2A-derived peptide as a defense molecule.

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来源期刊

Animal Gene Agricultural and Biological Sciences-Insect Science

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期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.