三体酒的细胞毒活性(略)直流。精油在肿瘤细胞系中的作用及其在相关死亡机制中的作用

IF 0.7 Q4 CHEMISTRY, MULTIDISCIPLINARY
I. Moro, F. A. Carvalho, Thais Fernanda Moreira, Felipe de Oliveira Souza, Alexander Alves da Silva, F. Politi, C. P. Soares, A. G. Santos
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引用次数: 0

摘要

本研究通过细胞毒性和细胞死亡测定的应用,评估了曲美杆菌精油(EO)及其成分在癌症治疗中的潜力。经气相色谱分析,该精油地上部分的主要成分为(E)-石竹烯(18.9%)、双环木烯(15.6%)和germacrene D(10.5%),(E)-石竹烯(11.5μg/mL)和石竹烯氧化物(>10.00μg/mL。对HepG2细胞株,三聚芽孢杆菌精油(10.4μg/mL)、α-胡木烯(17.1μg/mL。与MCF-10A细胞相比,EO(MCF-7和HepG2)、α-腐殖烯和(E)-石竹烯(MCF-7)以及石竹烯氧化物(HepG2。用精油、(E)-石竹烯、α-腐殖质和石竹烯氧化物进行的膜联蛋白-V和Hoechst/碘化丙啶测定显示,所有细胞系都存在凋亡和坏死机制。基于这些发现,偏苯三甲酸苯酯精油及其成分可以被认为是潜在的癌症治疗剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cytotoxic Activity of Baccharis trimera (Less.) DC. Essential Oil in Tumor Cell Lines and its Role in Associated Death Mechanisms
This study evaluated the potential of Baccharis trimera essential oil (EO) and its components in cancer therapy through the application of cytotoxicity and cell death assays. Using gas chromatography analysis, the major components of the aerial parts of the essential oil were identified as (E)-caryophyllene (18.9%), bicyclogermacrene (15.6%), and germacrene D (10.5%). Baccharis trimera essential oil (5.8 μg/mL) and α-humulene (7.8 μg/mL) presented strong cytotoxic activity, while (E)-caryophyllene (11.5 μg/mL) and caryophyllene oxide (> 100.0 μg/mL) showed moderate and low activities, respectively, against MCF-7 cell lines. Against HepG2 cell lines, B. trimera essential oil (10.4 μg/mL), α-humulene (17.1 μg/mL), and caryophyllene oxide (19.4 μg/mL) exhibited moderate activity, while (E)-caryophyllene (52.3 μg/mL) displayed low activity against HepG2 cell lines. The selectivity index values of EO (MCF-7 and HepG2), α-humulene and (E)-caryophyllene (MCF-7), and caryophyllene oxide (HepG2) were found between 1.1 and 2.8, compared with MCF-10A cells. The annexin-V and Hoechst / propidium iodide assays performed with essential oil, (E)-caryophyllene, α-humulene, and caryophyllene oxide showed apoptosis and necrosis mechanisms for all cell lines. Based on these findings, B. trimera essential oil and its components can be considered as potential therapeutic agents against cancer.
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来源期刊
Orbital: The Electronic Journal of Chemistry
Orbital: The Electronic Journal of Chemistry CHEMISTRY, MULTIDISCIPLINARY-
CiteScore
1.10
自引率
0.00%
发文量
25
审稿时长
10 weeks
期刊介绍: Orbital: The Electronic Journal of Chemistry is a quarterly scientific journal published by the Institute of Chemistry of the Universidade Federal de Mato Grosso do Sul, Brazil. Original contributions (in English) are welcome, which focus on all areas of Chemistry and their interfaces with Pharmacy, Biology, and Physics. Neither authors nor readers have to pay fees. The journal has an editorial team of scientists drawn from regions throughout Brazil and world, ensuring high standards for the texts published. The following categories are available for contributions: 1. Full papers 2. Reviews 3. Papers on Education 4. History of Chemistry 5. Short communications 6. Technical notes 7. Letters to the Editor The Orbital journal also publishes a number of special issues in addition to the regular ones. The central objectives of Orbital are threefold: (i) to provide the general scientific community (at regional, Brazilian, and worldwide levels) with a formal channel for the communication and dissemination of the Chemistry-related literature output by publishing original papers based on solid research and by reporting contributions which further knowledge in the field; (ii) to provide the community with open, free access to the full content of the journal, and (iii) to constitute a valuable channel for the dissemination of Chemistry-related investigations.
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