GA3介导的烟草3-羟基-3-甲基戊二酰辅酶a还原酶1（NtHMGR1）转录速率和mRNA稳定性的提高。

Q3 Medicine

Notulae Scientia Biologicae Pub Date : 2022-11-28 DOI:10.55779/nsb14411317

R. Manoharlal, Lucky Duhan, R. Pasrija, Saiprasad V. S. Gandra

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引用次数: 0

摘要

我们目前的研究评估了通过赤霉素A3（GA3）处理模式栽培烟草烟草烟草中观察到的3-羟基-3-甲基戊二酰辅酶A还原酶1（NtHMGR1）转录水平和伴随的功能活性增强的潜在分子机制。基于转录运行和虫草素追逐试验，我们的结果表明，用GA3引发烟草种子会相对显著地提高NtHMGR1的转录速率和mRNA稳定性。总之，我们的研究确定，GA3介导的转录和转录后调控是观察到的NtHMGR1转录水平增强并因此增强功能活性的机制之一。

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GA3 mediated enhanced transcriptional rate and mRNA stability of 3-hydroxy-3-methylglutaryl coenzyme a reductase 1 (NtHMGR1) in Nicotiana tabacum L.

Our present study evaluated the underlying molecular-mechanism(s) associated with the observed enhanced transcript levels and concomitant functional activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase 1 (NtHMGR1), a rate-limiting enzyme of cytosolic mevalonate (MVA) pathway of terpenoids biosynthesis, by gibberellin A3 (GA3) treatment in model cultivated tobacco, Nicotiana tabacum L. Based on the transcription run-on and cordycepin chase assays, our results demonstrated that tobacco seeds-priming with GA3 causes a relative and significantly enhanced transcriptional rate and mRNA stability of NtHMGR1. Taken together, our study established that GA3 mediated transcriptional and post-transcriptional regulatory control as one of the mechanisms for the observed enhanced transcript-levels and consequently enhanced functional activity of NtHMGR1.

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来源期刊

Notulae Scientia Biologicae Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (miscellaneous)

CiteScore

1.10

自引率

0.00%

发文量

审稿时长

12 weeks