凝固酶阴性葡萄球菌临床重要分离株耐利奈唑胺的分子特征,一项来自北方邦西部医院的研究

IF 2.1 Q2 MEDICINE, GENERAL & INTERNAL
Priyakshi Chaudhry, A. Pandey, P. Chaturvedi, Km. Sangita
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引用次数: 0

摘要

简介与目的:多药耐药凝固酶阴性葡萄球菌的出现是一个治疗难题。耐利奈唑胺类疾病(lrcon)呈显著上升趋势,全球患病率约为2%。氯霉素-氟苯尼考耐药基因的获得是导致耐药的最常见机制。本研究旨在确定临床上重要的凝固酶阴性葡萄球菌(con)耐利奈唑胺的分子特征。材料与方法:采用标准细菌学技术对临床分离的1061株con进行鉴定。对利奈唑胺进行药敏试验,区径为20 mm(利奈唑胺筛选阳性)的分离菌经全自动Vitek 2紧凑系统验证,MIC为8 μ g / mL。在表型证实的lrcon中检测到cfr基因。结果:2.5%临床重要的凝固酶阴性葡萄球菌对利奈唑胺耐药。溶血链球菌的耐药性最高(70%),其次是柯氏链球菌(22.2%)。在lrcon中,有78%的分离株检出cfr基因,主要见于溶血链球菌和柯氏链球菌。所有lrcon均为mrcon(甲氧西林耐药)。但所有菌株均对糖肽敏感。结论:据报道,临床样本中对包括利奈唑胺在内的许多重要抗菌素具有耐药性的con病例激增。cfr基因的存在是利奈唑胺耐药最常见的机制。早期和正确识别这些分离株并遵守感染控制方案将有助于获得更好的临床结果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular characterization of linezolid resistance in clinically significant isolates of coagulase negative Staphylococcus species, a hospital based study from Western Uttar Pradesh
Introduction and aim: The emergence of Multidrug-resistant Coagulase-negative Staphylococcus species is a therapeutic problem. Linezolid-resistant CoNS (LRCoNS) are on a significant rise, with a global prevalence of around 2%. The acquisition of cfr (chloramphenicol-florfenicol resistance) gene  is the commonest  mechanism leading to resistance. This study aimed to determine the molecular characterization of linezolid resistance in clinically significant isolates of Coagulase Negative Staphylococci (CoNS).   Material and Methods: The 1061 clinical  isolates of CoNS were identified by standard bacteriological technique. Antibiotic sensitivity test was performed to determine the susceptibility to linezolid and those isolates with zone diameter 20 mm (linezolid screen positive) were further confirmed by the automated Vitek 2 compact system and MIC 8 microgram per mL was resistant . The cfr gene was detected in phenotypically confirmed LRCoNS.   Results: Resistant to Linezolid was seen in 2.5% of clinically important isolates of Coagulase Negative Staphylococci. The resistance was maximum in S.hemolyticus (70%), followed by S.cohnii (22.2%). Among the LRCoNS, the overall cfr gene was detected in 78% of isolates, predominantly in S.hemolyticus and S. cohnii. All the LRCoNs were also MRCoNS (methicillin resistance). However, all isolates were susceptible to glycopeptides.   Conclusion: There has been a surge of CoNS being reported from clinical samples with resistance to many important antimicrobials, including linezolid. The presence of cfr gene is the most common mechanism of resistance to linezolid. Early and correct identification of these isolates and adherence to infection control protocols will help for better clinical outcomes.
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来源期刊
BioMedicine-Taiwan
BioMedicine-Taiwan MEDICINE, GENERAL & INTERNAL-
CiteScore
2.80
自引率
5.90%
发文量
21
审稿时长
24 weeks
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