有机酸与纯植物药微囊化混合物对体外双流连续培养系统瘤胃微生物群的影响

IF 3.3 3区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
R. Lobo, L. Roesch, E. Grilli, A. Faciola
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引用次数: 1

摘要

本研究的目的是在体外双流连续培养系统中评估饲粮中添加有机酸和纯植物药微胶囊混合物(mOAPBs)对瘤胃微生物组固体和液体相关微环境(分别为SAM和LAM)的影响。在8个发酵罐中培养瘤胃内容物,在基础饲粮中添加增加水平的mOAPBs (0;0.12;0.24;(0.36% DM),其中含有55.6%氢化和精制棕榈油、25%柠檬酸、16.7%山梨酸、1.7%百里香酚和1%香兰素。所有饲粮的营养成分相似(16.1 CP, 30.9 NDF, 32.0 starch, % DM基础)。适应期7 d后,在每个时期收集不同天数的样本,鉴定SAM和LAM的微生物组。mOAPB对α -、β -多样性和微生物丰度没有影响。SAM具有更大的细菌多样性,主成分分析表明它与LAM具有不同的细菌特征。此外,SAM还增加了碳水化合物降解微生物的丰度。综上所述,mOAPBs没有调节瘤胃微生物组。固体和液体相关微环境的微环境微生物群不同,SAM具有更多的碳水化合物降解微生物群。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of Microencapsulated Blend of Organic Acids and Pure Botanicals on the Ruminal Microbiota in an In Vitro Dual-Flow Continuous Culture System
The objective of the study was to evaluate the effects of dietary supplementation of a microencapsulated blend of organic acids and pure botanicals (mOAPBs) on the solid- and liquid-associated microenvironment (SAM and LAM, respectively) of the ruminal microbiome using an in vitro dual-flow continuous culture system. Ruminal content was incubated in eight fermenters and the basal diet was supplemented with increasing levels of mOAPBs (0; 0.12; 0.24; or 0.36% DM) which contained 55.6% hydrogenated and refined palm oil, 25% citric acid, 16.7% sorbic acid, 1.7% thymol, and 1% vanillin. All diets had a similar nutritional composition (16.1 CP, 30.9 NDF, and 32.0 starch, % DM basis). After 7 days of adaptation, a pooled sample across the days was collected in each period for identification of the microbiome of SAM and LAM. There was no effect of mOAPB on alpha-, beta-diversity, and microbial abundance. The SAM had a greater bacterial diversity and the principal component analysis demonstrated that it had a divergent bacterial profile from LAM. Additionally, SAM had an increased abundance of carbohydrate-degrading microorganisms. In summary, mOAPBs did not modulate the ruminal microbiome. The microenvironment microbiome of solid- and liquid-associated microenvironments were different, with SAM having a greater carbohydrate-degrading microorganism population.
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来源期刊
Fermentation-Basel
Fermentation-Basel BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
3.80
自引率
18.90%
发文量
594
审稿时长
7 weeks
期刊介绍:
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