差向突变因子作用下冬小麦细胞遗传学效应的发生

IF 0.5 Q4 BIOLOGY
V. Beiko, M. Nazarenko
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引用次数: 1

摘要

研究了0.01%、0.05%、0.10%和0.50%差向异构酶Triton-X-305在细胞水平上的影响特征,即鉴定第一代植物花粉活力的方法,以及对面包冬小麦种子发芽过程中生发根初级分生组织细胞染色体畸变的细胞学分析。这一方向的研究使我们能够显著改善对环境中相同类型物质的监测,并在DNA水平上预测其作用的性质。研究了乌克兰选择产生的Podolyanka和Spivanka基因型以及法国选择的Altigo、Courtot、Lyrik和Flamenko品种。选择这些基因型是为了表征复杂遗传模式化合物在一系列浓度下可能的基因型-突变相互作用,最大对比度考虑到可能的高位点效应。本研究的主要目的是确定Triton X-305试剂在细胞水平上影响的特异性,并确定充分显示该物质对随后诱导生物多样性和增强细胞水平遗传变异性的影响的参数。研究了不同浓度对花粉不育性的影响、染色体重排的总频率、染色体重排谱(包括片段和双片段、单桥和双桥、微核和滞后染色体)等指标。片段与桥的比率作为活性因子性质的指标,具有两个或两个以上重排的细胞数量作为该物质遗传毒性的指标已经确定。因此,与其他具有诱变性质的因素不同,差向突变原对染色体重排总频率的影响明显较弱,同时改变了获得的畸变率,有利于微核和滞后染色体。此外,其他参数被确定为对识别特定的影响因素更有意义,并且浓度差异的对比度较小。生育率也有不太显著的下降,然而,这个参数高度依赖于来源材料。未来,我们打算通过对后代获得的材料进行视觉分析,以及通过生物化学分析、产量质量分析和形态计量学来评估变异性,主要是遗传性的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Occurrence of cytogenetic effects under the action of epimutagen in winter wheat
Research on studying the features of 0.01%, 0.05%, 0.10% and 0.50% epimutagen Triton-X-305 impact at the cellular level, which means the way of identifying the viability of pollen in plants of the first generation, and cytological analysis of chromosomal aberrations in the cells of the primary meristem of germinal roots during germination of bread winter wheat seeds have been conducted. Research in this direction allows us to significantly improve the monitoring of same type of substances in the environment and to forecast the nature of their action at the DNA-level. Genotypes Podolyanka and Spivanka created by Ukrainian selection and varieties of French selection Altigo, Courtot, Lyrik, Flamenko have been studied. These genotypes were selected in order to characterize possible genotype-mutagenic interactions for a compound of complex hereditary pattern for a range of concentrations with maximum contrast taking into account the possible high site-specific effect. The main purpose of this research was to identify the specificity of impact of the agent Triton X-305 at the cellular level and identify parameters fully showing the effect of this substance on the subsequent induction of biodiversity and the enhancement of hereditary variability at the cellular level. Such indicators as pollen sterility effected by various concentrations, the total frequency of chromosomal rearrangements, the spectrum of chromosomal rearrangements, including fragments and double fragments, single and double bridges, micronuclei and lagging chromosomes have been investigated. The ratio of fragments to bridges as an indicator of the active factor nature, the number of cells with two or more rearrangements as an indicator of genetic toxicity of this substance has been established. As a result, a significantly weaker effect of the epimutagen on the overall frequency of chromosomal rearrangement has been shown unlike other factors of a mutagenic nature, at the same time changing the ratio of the obtained aberrations in favour of micronuclei and lagging chromosomes. In addition, other parameters are determined as more meaningful for identifying a specific agent of impact, and the differences in concentrations are less contrasting. There is also a less significantly decreasing fertility, however, this parameter is highly dependent on the source material. In the future, we intend to assess the variability, primarily of a hereditary nature, by the way of a visual analysis of the obtained material in subsequent generations, as well as through biochemical analysis, yield qualities analysis and morphometry of the obtained material.
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来源期刊
CiteScore
0.90
自引率
0.00%
发文量
25
审稿时长
10 weeks
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