从生物垃圾到骨替代品

IF 1.6 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Chemical and Biochemical Engineering Quarterly Pub Date : 2020-07-31 DOI:10.15255/cabeq.2020.1783

A. Ressler, A. Gudelj, Karla Zadro, M. Antunović, M. Cvetnić, M. Ivanković, H. Ivanković

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引用次数: 15

摘要

壳聚糖/羟基磷灰石（CS/HAp）复合支架可以模拟骨的纳米复合结构。生物羟基磷灰石（HAp）含有多种离子，在骨生长中起着至关重要的作用。本工作的目的是合成仿生羟基磷灰石，并制备基于壳聚糖的复合支架，其中以蛋壳、贝壳和墨鱼骨为原料合成羟基磷灰石。粉末由纳米结构的缺钙HAp和无定形磷酸钙（ACP）组成。在所制备的粉末中，由于使用Ca2+离子的生物前体，检测到Sr2+、Mg2+和Na+离子。采用冷冻凝胶技术制备了高孔CS/HAp结构。CS/HAp支架显示出高度多孔的结构，具有非常好的互连孔和均匀分散的HAp颗粒。CS/HAp支架的MTT测定显示没有毒性，活/死测定证实了接种细胞的良好生存能力和增殖能力。

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From Bio-waste to Bone Substitute

Nanocomposite structure of the bone can be mimicked by chitosan/hydroxyapatite (CS/HAp) composite scaffold. Biological hydroxyapatite (HAp) contains various ions, which have a crucial role in bone growth. The aim of the present work was to synthesize biomimetic hydroxyapatite and prepare composite scaffolds based on chitosan, where HAp was synthesised from hen eggshells, seashells and cuttlefish bone. The powders were composed of nano-structured calcium deficient HAp and amorphous calcium phosphate (ACP). In the as-prepared powders, Sr2+, Mg2+ and Na+ ions were detected as a result of using biogenic precursor of Ca2+ ions. Highly porous CS/HAp structures have been prepared by freeze-gelation technique. The CS/HAp scaffolds have shown highly porous structure with very well interconnected pores and homogeneously dispersed HAp particles. The MTT assay of CS/HAp scaffolds has shown no toxicity, and the live/dead assay has confirmed good viability and proliferation of seeded cells.

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来源期刊

Chemical and Biochemical Engineering Quarterly 工程技术-工程：化工

CiteScore

2.70

自引率

6.70%

发文量

审稿时长

>12 weeks

期刊介绍： The journal provides an international forum for presentation of original papers, reviews and discussions on the latest developments in chemical and biochemical engineering. The scope of the journal is wide and no limitation except relevance to chemical and biochemical engineering is required. The criteria for the acceptance of papers are originality, quality of work and clarity of style. All papers are subject to reviewing by at least two international experts (blind peer review). The language of the journal is English. Final versions of the manuscripts are subject to metric (SI units and IUPAC recommendations) and English language reviewing. Editor and Editorial board make the final decision about acceptance of a manuscript. Page charges are excluded.