光照条件、瓶封和培养时间对石斛萌发和离体发育的影响。

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-12-01 DOI:10.1590/2447-536x.v28i4.2515

Isabella de Souza Ribeiro, L. M. Ribeiro, J. S. Soares, J. Ramos, J. C. Sorgato

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引用次数: 1

摘要

摘要兰科植物生产的障碍之一是种子繁殖问题。因此，在离体培养中，光照和瓶封类型是影响兰花萌发和初期发育的因素。因此，本研究的目的是评价石斛萌发和离体发育初期的光照条件、瓶封方式和评价时间。播种后，根据烧瓶的密封，将培养物置于两种辐照条件下:7.0µmol m-2 s-1(螺纹盖)和19.0µmol m-2 s-1(塑料薄膜)，两种光照条件:白色荧光灯和红色荧光灯。在30、60、90 d时，分别测定1、2、3、4期原球茎的发芽率和成活率。DIC在播种后30天和60天采用细分小区方案，90天采用2 × 2因子方案。白色荧光灯覆盖塑料薄膜时萌发率最高(70.33%)，30 d时萌发率最高(70.13%)。90 d时使用塑料薄膜覆盖和白色荧光灯时存活率最高(100%)。在带螺纹灯头的红色荧光灯下繁殖体发育最大，达到第4期(9.55%)。

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Light condition, flask sealing, and cultivation time on the germination and initial in vitro development of Dendrobium nobile Lindl.

Abstract One of the obstacles for the production of Orchidaceae plants is the seed propagation. Thus, in in vitro cultivation, luminosity and the type of sealing of the bottles are factors that influence germination and initial development of orchids. Therefore, the objective was to evaluate the light condition, the type of sealing of the bottles and the evaluation time in germination and initial in vitro development of Dendrobium nobile Lindl. After sowing, cultures were subjected to two irradiance conditions according to the sealing of the flasks: 7.0 µmol m-2 s-1 (threaded cap) and 19.0 µmol m-2 s-1 (plastic film) and two light conditions: white fluorescent lamp and red fluorescent lamp. At 30, 60 and 90 days, the percentage of germination, survival, of protocorms in stage 1, stage 2, stage 3 and stage were evaluated 4. DIC was used, in a sub-divided plot scheme for 30 and 60 days after sowing and in a 2 x 2 factorial scheme at 90 days. The highest germination percentage was observed when using white fluorescent lamp with a plastic film cover (70.33%) and at 30 days (70.13%). The highest survival percentage (100%) was observed when using plastic film cover and white fluorescent lamp at 90 days. The greatest development of propagules, reaching stage 4 was verified in the red fluorescent lamp with threaded cap (9.55%).

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.