基于同工酶的陆生兰花(Cephalanthera subaphylla)分类等级评价

IF 0.8 Q4 PLANT SCIENCES
M. Chung, S. Son, J. Chung, J. López‐Pujol, T. Yukawa, M. Chung
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引用次数: 0

摘要

陆生小叶兰花Cephalanthera subaphylla Miyabe & Kudo的分类等级一直存在争议,因为它被视为C. erecta (Thunb)下的一个种或一个次种分类单元。布卢姆(C。erecta var. aphylla (Miyabe & Kudo) Ohwi和C. erecta f. aphylla (Miyabe & Kudo) M. Hiro]。同工酶标记,传统上用于划分种界,在这里被用来获得信息,以确定葡萄球菌的分类地位。为此,我们采集了原产于朝鲜半岛的头类动物(Cephalanthera) 5个分类群中的3个种群(共15个种群)。直立,C. falcata(拇指)Blume, C. longifolia (L.)Fritsch, and C. subaphylla]。在已确定的20个基因座中,有3个在5个物种中是单态的(Dia-2、Pgi-1和Tpi-1)。除长苞草外,其余4种间均无同工酶变异。在这17个多态位点所包含的51个等位基因中,14个位点的27个等位基因中的每一个都是单一物种所特有的。因此,我们发现10个种对之间Nei’s遗传特性(I)的平均值较低(直立c与长叶c的I = 0.250,镰形c与长苞c的I = 0.603),而葡萄球菌与其他种的遗传差异明显(葡萄球菌与长叶c的I = 0.349,葡萄球菌与镰形c的I = 0.400)。这些结果清楚地表明,C. subaphylla与包括C. erecta在内的朝鲜半岛原生头类动物的任何其他分类群都没有遗传关系。在主坐标分析(PCoA)中,葡萄球菌不仅与镰刀菌、长苞菌和长叶菌距离较远,而且与直立菌的距离也较远。最后,K = 5是贝叶斯方法的最佳聚类方案,5个聚类精确对应5个分类群。因此,我们的同工酶结果强烈表明,葡萄球菌值得物种的排名。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of the taxonomic rank of the terrestrial orchid Cephalanthera subaphylla based on allozymes
The taxonomic rank of the tiny-leaved terrestrial orchid Cephalanthera subaphylla Miyabe & Kudo has been somewhat controversial, as it has been treated as a species or as an infraspecific taxon, under C. erecta (Thunb.) Blume [C. erecta var. subaphylla (Miyabe & Kudo) Ohwi and C. erecta f. subaphylla (Miyabe & Kudo) M. Hiro]. Allozyme markers, traditionally employed for delimiting species boundaries, are used here to gain information for determining the taxonomic status of C. subaphylla. To do this, we sampled three populations of five taxa (a total of 15 populations) of Cephalanthera native to the Korean Peninsula [C. erecta, C. falcata (Thunb.) Blume, C. longibracteata Blume, C. longifolia (L.) Fritsch, and C. subaphylla]. Among 20 putative loci resolved, three were monomorphic (Dia-2, Pgi-1, and Tpi-1) across the five species. Apart from C. longibracteata, there was no allozyme variation within the remaining four species. Of the 51 alleles harbored by these 17 polymorphic loci, each of the 27 alleles at 14 loci was unique to a single species. Accordingly, we found low average values of Nei’s genetic identities (I) between ten species pairs (from I = 0.250 for C. erecta versus C. longifolia to I = 0.603 for C. falcata vs. C. longibracteata), with C. subaphylla being genetically clearly differentiated from the other species (from I = 0.349 for C. subaphylla vs. C. longifolia to 0.400 for C. subaphylla vs. C. falcata). These results clearly indicate that C. subaphylla is not genetically related to any of the other taxa of Cephalanthera that are native to the Korean Peninsula, including C. erecta. In a principal coordinate analysis (PCoA), C. subaphylla was positioned distant not only from C. falcata, C. longibracteata, and C. longifolia, but also from C. erecta. Finally, K = 5 was the best clustering scheme using a Bayesian approach, with five clusters precisely corresponding to the five taxa. Thus, our allozyme results strongly suggest that C. subaphylla merits the rank of species.
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来源期刊
KOREAN JOURNAL OF PLANT TAXONOMY
KOREAN JOURNAL OF PLANT TAXONOMY Agricultural and Biological Sciences-Plant Science
CiteScore
1.50
自引率
40.00%
发文量
23
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