疏水性抗癌药紫杉醇的简单分光光度评价方法

K. Sugo, M. Ebara
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引用次数: 3

摘要

在这项工作中,我们展示了一种简单的分光光度法来更准确地定量和测量紫杉醇(PTX)浓度。当与水性溶剂混合时,PTX不能精确定量,并且具有发生晶体沉淀的风险。PTX可能与水性溶剂发生多次相互作用,并由于其低溶解度而进入过饱和状态。因此,在临床使用之前,需要一种定量的方法来测量PTX以进行质量控制。尽管迄今为止已经报道了几种高效液相色谱(HPLC)方法,但并不是所有的医疗机构都有具有这种HPLC设备和分析技术的临床实验室。光谱学是一种简单方便的方法;然而,校准标准品是用有机溶剂制备的,如甲醇和乙腈,当与PTX混合时,会引起溶剂效应,导致结果不准确。我们生成了不同浓度(40%、50%、60%、70%、80%、90%和100%)甲醇下PTX的校准曲线,并评估了HPLC结果的相对误差。定量PTX的最佳甲醇浓度为65.8%,这对应于最小的相对误差。检测限和定量限分别为0.030μg/mL和0.092μg/mL。即使当加入聚氧乙烯蓖麻油和无水乙醇时,也可以预测PTX浓度,如在市售的PTX制剂中一样,在混合后用盐水稀释32倍。我们的研究结果表明,当在甲醇/水混合物中制备时,使用校准曲线可以更准确地定量PTX,而不需要特殊的设备或技术。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A simple spectrophotometric evaluation method for the hydrophobic anticancer drug paclitaxel
In this work, we demonstrate a simple spectrophotometry approach to more accurately quantify and measure paclitaxel (PTX) concentrations. PTX cannot be precisely quantified when mixed with an aqueous solvent, and carries the risk of undergoing crystal precipitation. It is likely that PTX undergoes numerous interactions with aqueous solvents and enters a supersaturated state due to its low solubility. Therefore, a quantitative method is required to measure PTX for quality control before clinical use. Although several high-performance liquid chromatography (HPLC) methods have been reported to date, not all medical facilities have a clinical laboratory with such HPLC devices and analysis techniques. Spectroscopy is a simple and convenient method; however, calibration standards are prepared with an organic solvent, such as methanol and acetonitrile, which, when mixed with PTX, can cause solvent effects that lead to inaccurate results. We generated a calibration curve of PTX at various concentrations (40%, 50%, 60%, 70%, 80%, 90% and 100%) of methanol and evaluated the relative error from HPLC results. The optimum methanol concentration for quantification of PTX was 65.8%, which corresponded to the minimum relative error. The detection limit and quantification limit were 0.030 μg/mL and 0.092 μg/mL, respectively. It was possible to predict the PTX concentration even when polyoxyethylene castor oil and anhydrous ethanol were added, as in the commercially available PTX formulation, by diluting 32-fold with saline after mixing. Our findings show that PTX can be more accurately quantified using a calibration curve when prepared in a methanol/water mixture without the need for special devices or techniques.
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