绿色合成的双星草根提取物氧化锌纳米颗粒(第二篇)减轻自由基和糖尿病相关酶

Q3 Engineering

Nanoscience and Nanotechnology - Asia Pub Date : 2020-11-30 DOI:10.2174/2210681210666200117150727

F. Balogun, A. Ashafa

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引用次数: 1

摘要

生态友好型金属纳米结构合成方法的出现不断得到广泛的接受。研究了生物合成氧化锌纳米颗粒对游离自由基和糖水解酶的影响。采用紫外可见光谱、透射电子显微镜、傅里叶变换红外光谱(FTIR)、能量色散光谱和x射线衍射技术(XRD)获得了表征的纳米粒子DaZnONPs (Dicoma anomalala zinc oxide nanoparticles)。采用标准方法测定了合成的纳米结构对1,1-二苯基-2-吡啶肼(DPPH)、2,2 ' -氮基-双(3-乙基苯并噻唑啉-6-磺酸(ABTS)、金属螯合、α -淀粉酶和α -葡萄糖苷酶的活性。DaZnONPs被观察到是稳定的，大多数是立方体形状，在纳米尺寸范围内。在386 nm处观察到DaZnONPs的最佳吸收。FTIR分析表明存在由生物碱、黄酮类、单宁和皂苷产生的官能团(在早期的报道中检测到)，并表明ZnONPs可能成核和稳定性。xr结果显示了DaZnONPs和标准ZnO光谱的相似模式，揭示了粒子在纳米范围内的六方体晶体性质。DaZnONPs对DPPH (0.54 μgmL-1)和α -淀粉酶(104.34 μgmL-1)的抑制作用优于槲皮素(349.98 μgmL-1)和阿卡波糖(594 μgmL-1)。54μgmL-1。同时，daznonps (30.41 μgmL-1)与槲皮素(27.81 μgmL-1)的金属螯合作用不显著(p < 0.05)。DaZnOnPs对α -淀粉酶和α -葡萄糖苷酶具有非竞争性抑制作用。DaZnONPs(相对于散装提取物)可以作为可能的抗氧化和抗高血糖药物，减轻自由基和高血糖的不利影响。

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Green-synthesized Zinc Oxide Nanoparticles from Aqueous Root Extract of Dicoma anomala (Sond.) Mitigates Free Radicals and Diabetes-linked Enzymes

The emergence of eco-friendly methods for the synthesis of metallic nanostructures has continued to receive wider acceptance. The study investigated the effect of biologically-synthesized ZnO nanoparticles on free radicals and carbohydrate-hydrolyzing enzymes. The characterized nanoparticles, DaZnONPs (Dicoma anomala zinc oxide nanoparticles) were obtained using ultraviolet-visible spectroscopy, transmission electron microscopy, Fourier- transform infrared spectroscopy (FTIR), energy dispersive spectroscopy, and X-ray diffraction technique (XRD). The activity of the synthesized nanostructures against 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), metal chelating, alpha-amylase and alpha-glucosidase was determined using standard methods. DaZnONPs were observed to be stable, mostly cubical in shape and within the nanometre size range. Optimum absorption of DaZnONPs was observed at 386 nm. The FTIR analysis indicated the presence of functional groups arising from alkaloids, flavonoids, tannins, and saponins (detected in earlier reports) and indicate potential nucleation and stability of the ZnONPs. XRD result depicted similar patterns of DaZnONPs and standard ZnO spectra, revealing a hexagonal and crystalline nature of the particles in nanometre range as shown by the obtained peaks. DaZnONPs inhibited DPPH (0.54 μgmL-1 and alpha-amylase (104.34 μgmL-1 better than quercetin (349.98 μgmL-1 and acarbose (594. 54 μgmL-1. Meanwhile, the metal chelating effect of DaZnONPs (30.41 μgmL-1 was observed to be insignificantly (p>0.05) at par with quercetin (27.81 μgmL-1. The kinetics of alpha-amylase and alpha-glucosidase enzymes by DaZnOnPs was observed to be non-competitive inhibitions. DaZnONPs (as against the bulk extract) could be explored as possible antioxidative and antihyperglycaemic agents mitigating the adverse effects of free radicals and hyperglycaemia.

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来源期刊

Nanoscience and Nanotechnology - Asia Engineering-Engineering (all)

CiteScore

1.90

自引率

0.00%

发文量

期刊介绍： Nanoscience & Nanotechnology-Asia publishes expert reviews, original research articles, letters and guest edited issues on all the most recent advances in nanoscience and nanotechnology with an emphasis on research in Asia and Japan. All aspects of the field are represented including chemistry, physics, materials science, biology and engineering mainly covering the following; synthesis, characterization, assembly, theory, and simulation of nanostructures (nanomaterials and assemblies, nanodevices, nano-bubbles, nano-droplets, nanofluidics, and self-assembled structures), nanofabrication, nanobiotechnology, nanomedicine and methods and tools for nanoscience and nanotechnology.