临床分离Pantoea sp .部分毒力和抗生素基因的分子鉴定和检测

IF 2.1 Q2 MEDICINE, GENERAL & INTERNAL
Noor Hameed Shahab, Suha Maher Abed, Maan Hasan Salih
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引用次数: 0

摘要

简介与目的:Pantoea属革兰氏阴性菌,属Erwiniaceae科。这些细菌是已知会引起几种人类临床感染的机会性病原体。本研究的目的是基于16S rRNA测序研究Pantoea spp.的流行和鉴定,并检测临床病例分离样品中抗生素耐药和毒力基因的存在。材料与方法:对Pantoea spp.进行鉴定,提取分离物DNA,用PCR扩增16s rRNA。使用特定引物评估Pantoea分离株的毒力(EsaI和ompA)和抗生素(bacA, oqxB, qnrA和ermB)基因的存在。结果:根据16S rRNA测序结果,记录到3株新菌株。所有分离株(100%)均存在毒力基因Esal, 15/16(94%)分离株存在ompA基因。所有菌株中均检出耐药基因oqxB(100%),其次是bacA(87.5%)、qnrA(75%)和ermB(37%)。结论:从临床标本中分离到的泛菌具有一定的毒力和抑菌基因,具有一定的致病性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular identification and detection of some virulence and antibiotic genes in Pantoea spp. isolated from clinical samples
Introduction and Aim: Pantoea species, are Gram negative bacteria that belong to the family Erwiniaceae. These bacteria are opportunistic pathogens known to cause several clinical infections in humans. The purpose of current research was to study the prevalence and identification of Pantoea spp. based on 16S rRNA sequencing, as well as detect the presence of antibiotic resistance and virulence genes in samples isolated from clinical cases.   Materials and Methods: For the identification of Pantoea spp., DNA extracted from isolates were subjected to 16s rRNA amplification by PCR. Pantoea isolates were assessed for the presence of virulence (EsaI and ompA), and antibiotic (bacA, oqxB, qnrA, and ermB) genes using specific primers.   Results: Three new strains were recorded based on results of 16S rRNA sequencing. The virulence gene Esal was prevalent in all isolates (100%) tested while 15/16 (94%) of isolates had ompA genes. The antibiotic-resistant gene oqxB was found in all isolates (100%), followed by bacA (87.5%), qnrA (75%), and ermB (37%) genes.   Conclusion: Pantoea spp isolated from clinical samples were seen to harbor virulence as well as antimicrobial genes indicating the isolates to be pathogenic.
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来源期刊
BioMedicine-Taiwan
BioMedicine-Taiwan MEDICINE, GENERAL & INTERNAL-
CiteScore
2.80
自引率
5.90%
发文量
21
审稿时长
24 weeks
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