粘土矿物改变镉对亮氨酸氨肽酶活性的毒性作用

IF 2.8 4区 工程技术 Q2 CHEMISTRY, APPLIED
Shunyu Huang, Jingji Li, Ji-peng Wang
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引用次数: 2

摘要

土壤亮氨酸氨基肽酶(LAP)是一种参与微生物获取氮的水解酶。在污染土壤中,LAP活性不仅受重金属类型和浓度的影响,还受酶形态的影响。本文研究了土壤LAP和纯化LAP对镉(Cd)的抑制程度和机制。我们还研究了蒙脱土和高岭土对LAP和受Cd污染的LAP的影响。结果表明,Cd对LAP活性的抑制作用随着Cd浓度的增加而增强,Cd对LAP的抑制作用是非竞争性的。黏土矿物的加入降低了LAP活性和最大反应速率(vmax),与Cd的存在无关。蒙脱土降低了LAP对底物的亲和力(K m),而高岭石提高了LAP对底物的亲和力。黏土矿物固定化LAP对Cd污染的抗性较游离LAP有所提高。本研究结果有助于了解重金属对土壤酶的毒性作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Clay Minerals Change the Toxic Effect of Cadmium on the Activities of Leucine Aminopeptidase
Soil leucine aminopeptidase (LAP) is a hydrolytic enzyme involved in the acquisition of nitrogen by microorganisms. In contaminated soils, LAP activity is affected not only by the type and concentration of heavy metals but also by the form of enzyme. Here, we investigated the degree and mechanism of cadmium (Cd) inhibition of soil LAP and purified LAP. We also examined the effect of montmorillonite and kaolinite on LAP and LAP contaminated with Cd. The results showed that Cd inhibition of LAP activity increased with increasing Cd concentration and that Cd exerted noncompetitive inhibition of LAP. The addition of clay minerals decreases LAP activity and the maximum reaction rate ( V max ), regardless of the presence of Cd. Montmorillonite decreases the affinity of LAP to the substrate ( K m ), while kaolinite increases the affinity of LAP to the substrate. The clay mineral-immobilized LAP showed an increase in resistance to Cd contamination compared with the free LAP. The results obtained in this study may aid in understanding the toxic effects of heavy metals on soil enzymes.
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来源期刊
Adsorption Science & Technology
Adsorption Science & Technology 工程技术-工程:化工
CiteScore
5.00
自引率
10.30%
发文量
181
审稿时长
4.5 months
期刊介绍: Adsorption Science & Technology is a peer-reviewed, open access journal devoted to studies of adsorption and desorption phenomena, which publishes original research papers and critical review articles, with occasional special issues relating to particular topics and symposia.
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