首次从石油污染生态系统中分离到可生物降解的多环芳烃

IF 1.3 Q4 ENVIRONMENTAL SCIENCES
S. Hosseini, Davood Azadi, Abdorrahim Absalan
{"title":"首次从石油污染生态系统中分离到可生物降解的多环芳烃","authors":"S. Hosseini, Davood Azadi, Abdorrahim Absalan","doi":"10.34172/ehem.2022.11","DOIUrl":null,"url":null,"abstract":"Background: Polycyclic aromatic hydrocarbons (PAHs) have detrimental effects on human, ecosystem, and biodiversity. Bioremediation is an option that has been used to remediate and reduce the risk of contaminants such as PAHs. Microorganisms are readily available to screen and can be rapidly identified to be used in many extreme environmental conditions. Mycobacteria have a great potential for the production of bioactive compound, which have degradation activity. Due to this issue, and also, as there is no study conducted on the biodiversity of biodegradable Mycobacterium in Markazi province, the present study aimed to assess the isolation and identification of biodegradable Mycobacterium species from diverse Markazi province ecosystems. Methods: Mycobacterium were screened from a total of 30 soil, water, and sludge samples from the oil-polluted ecosystems of Markazi province and characterized to the genus and species level by applying molecular and conventional microbiological assay including the PCR amplification and sequence analysis of 16SrRNA and hsp65 genes. The growth rate in the presence of PAHs, turbidometry, and high performance liquid chromatography (HPLC) were used to determine their bioremediation capability. Results: In total, 6 Mycobacterium isolates (20%) were screened from 30 samples, which belonged to two species of Mycobacterium consisting of M. porcinum (4 isolates) and M. celeriflavum (2 isolates). The strains of M. porcinum and M. celeriflavum could degrade 70% and 90% of 1 mg/L PAH solution in 7 days. Conclusion: According to the results, the M. porcinum and M. celeriflavum have a significant capability to biodegrade the PAHs. Therefore, more investigations are recommended for separation and applicational use of the mycobacterium species for bioremediation of PAHs.","PeriodicalId":51877,"journal":{"name":"Environmental Health Engineering and Management Journal","volume":null,"pages":null},"PeriodicalIF":1.3000,"publicationDate":"2022-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"First isolation of biodegradable polycyclic aromatic hydrocarbons Mycobacterium porcinum and Mycobacterium celeriflavum from oil-polluted ecosystems\",\"authors\":\"S. Hosseini, Davood Azadi, Abdorrahim Absalan\",\"doi\":\"10.34172/ehem.2022.11\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background: Polycyclic aromatic hydrocarbons (PAHs) have detrimental effects on human, ecosystem, and biodiversity. Bioremediation is an option that has been used to remediate and reduce the risk of contaminants such as PAHs. Microorganisms are readily available to screen and can be rapidly identified to be used in many extreme environmental conditions. Mycobacteria have a great potential for the production of bioactive compound, which have degradation activity. Due to this issue, and also, as there is no study conducted on the biodiversity of biodegradable Mycobacterium in Markazi province, the present study aimed to assess the isolation and identification of biodegradable Mycobacterium species from diverse Markazi province ecosystems. Methods: Mycobacterium were screened from a total of 30 soil, water, and sludge samples from the oil-polluted ecosystems of Markazi province and characterized to the genus and species level by applying molecular and conventional microbiological assay including the PCR amplification and sequence analysis of 16SrRNA and hsp65 genes. The growth rate in the presence of PAHs, turbidometry, and high performance liquid chromatography (HPLC) were used to determine their bioremediation capability. Results: In total, 6 Mycobacterium isolates (20%) were screened from 30 samples, which belonged to two species of Mycobacterium consisting of M. porcinum (4 isolates) and M. celeriflavum (2 isolates). The strains of M. porcinum and M. celeriflavum could degrade 70% and 90% of 1 mg/L PAH solution in 7 days. Conclusion: According to the results, the M. porcinum and M. celeriflavum have a significant capability to biodegrade the PAHs. Therefore, more investigations are recommended for separation and applicational use of the mycobacterium species for bioremediation of PAHs.\",\"PeriodicalId\":51877,\"journal\":{\"name\":\"Environmental Health Engineering and Management Journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2022-03-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Environmental Health Engineering and Management Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.34172/ehem.2022.11\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Health Engineering and Management Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.34172/ehem.2022.11","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
引用次数: 1

摘要

背景:多环芳烃(PAHs)对人类、生态系统和生物多样性都有不利影响。生物修复是一种已被用于修复和减少多环芳烃等污染物风险的选择。微生物很容易筛选,可以快速识别,在许多极端环境条件下使用。分枝杆菌具有生产具有降解活性的生物活性化合物的巨大潜力。鉴于这一问题,同时由于目前还没有对马尔卡兹省可生物降解分枝杆菌的生物多样性进行研究,本研究旨在评估从马尔卡兹省不同生态系统中分离和鉴定可生物降解分枝杆菌的物种。方法:从Markazi省石油污染生态系统的30份土壤、水和污泥样品中筛选分枝杆菌,采用分子和常规微生物学方法,包括16SrRNA和hsp65基因的PCR扩增和序列分析,对其进行属和种水平的鉴定。采用浊度法和高效液相色谱法测定其生物修复能力。结果:从30份样本中共筛选出6株分枝杆菌(占20%),分别属于猪分枝杆菌(4株)和芹菜分枝杆菌(2株)。猪分枝杆菌和芹菜分枝杆菌在7天内对1 mg/L多环芳烃溶液的降解率分别为70%和90%。结论:猪支链菌和芹菜支链菌对多环芳烃具有明显的生物降解能力。因此,在多环芳烃生物修复中,分枝杆菌的分离和应用有待进一步研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
First isolation of biodegradable polycyclic aromatic hydrocarbons Mycobacterium porcinum and Mycobacterium celeriflavum from oil-polluted ecosystems
Background: Polycyclic aromatic hydrocarbons (PAHs) have detrimental effects on human, ecosystem, and biodiversity. Bioremediation is an option that has been used to remediate and reduce the risk of contaminants such as PAHs. Microorganisms are readily available to screen and can be rapidly identified to be used in many extreme environmental conditions. Mycobacteria have a great potential for the production of bioactive compound, which have degradation activity. Due to this issue, and also, as there is no study conducted on the biodiversity of biodegradable Mycobacterium in Markazi province, the present study aimed to assess the isolation and identification of biodegradable Mycobacterium species from diverse Markazi province ecosystems. Methods: Mycobacterium were screened from a total of 30 soil, water, and sludge samples from the oil-polluted ecosystems of Markazi province and characterized to the genus and species level by applying molecular and conventional microbiological assay including the PCR amplification and sequence analysis of 16SrRNA and hsp65 genes. The growth rate in the presence of PAHs, turbidometry, and high performance liquid chromatography (HPLC) were used to determine their bioremediation capability. Results: In total, 6 Mycobacterium isolates (20%) were screened from 30 samples, which belonged to two species of Mycobacterium consisting of M. porcinum (4 isolates) and M. celeriflavum (2 isolates). The strains of M. porcinum and M. celeriflavum could degrade 70% and 90% of 1 mg/L PAH solution in 7 days. Conclusion: According to the results, the M. porcinum and M. celeriflavum have a significant capability to biodegrade the PAHs. Therefore, more investigations are recommended for separation and applicational use of the mycobacterium species for bioremediation of PAHs.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
2.40
自引率
37.50%
发文量
17
审稿时长
12 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信