病毒介导的基因转导技术在眼科研究中的应用

Q4 Medicine

中华眼底病杂志 Pub Date : 2020-02-25 DOI:10.3760/CMA.J.ISSN.1005-1015.2020.02.019

Lijie Dong, Hui Zhang, Qiong Wang, Y. Hong

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引用次数: 0

摘要

随着分子生物学技术的进步和遗传学的发展，病毒载体系统不断完善和优化。病毒载体系统已逐渐成为眼科基因治疗的最佳载体之一。腺病毒载体具有瞬时表达的特点，在降低角膜免疫反应中起着重要作用。慢病毒载体具有稳定高效的特点，可以在体内长时间缓慢表达。腺相关病毒载体具有免疫原性低、高效、精确的特点，可感染多种视网膜细胞。腺相关病毒载体和CRISPR-Cas9的联合使用为眼科遗传疾病的精确治疗提供了新的方法。病毒载体的出现显著增加了基因治疗的潜力，并比传统疗法具有无与伦比的优势。我们有理由相信，在不久的将来，基于病毒的基因转导技术将很快实现临床应用，届时将解决大量眼科难题。关键词：基因转移，横向；评审；病毒载体

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Application of virus-mediated gene transduction technology in ophthalmology research

With the advancement of molecular biology technology and the development of genetics, the viral vector system has been continuously improved and optimized. The viral vector system has gradually become one of the best carriers in ophthalmic gene therapy. Adenovirus vector has the characteristics of transient expression and plays an important role in reducing corneal immune response. Lentiviral vector has the characteristics of stable and high efficiency and can be expressed slowly in the body for a long time.Adeno-associated virus vector has the characteristics of low immunogenicity, high efficiency and precision and can infect a variety of retinal cells. The combined use of adeno-associated virus vector and CRISPR-Cas9 provides new methods for precise treatment of ophthalmic genetic diseases. The advent of viral vectors has significantly increased the potential of gene therapy and has unparalleled advantages over traditional therapies. We have reason to believe that virus-based gene transduction technology will soon achieve clinical application in the near future, and a large number of difficult ophthalmic problems will be solved by then. Key words: Gene transfer, horizontal; Review; Viral vector

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来源期刊

中华眼底病杂志 Medicine-Ophthalmology

CiteScore

0.40

自引率

0.00%

发文量

5383

期刊介绍： Chinese Journal of Ocular Fundus Diseases is the only scientific journal in my country that focuses on reporting fundus diseases. Its purpose is to combine clinical and basic research, and to give equal importance to improvement and popularization. It comprehensively reflects the leading clinical and basic research results of fundus disease disciplines in my country; cultivates professional talents in fundus disease, promotes the development of fundus disease disciplines in my country; and promotes academic exchanges on fundus disease at home and abroad. The coverage includes clinical and basic research results of posterior segment diseases such as retina, uveal tract, vitreous body, visual pathway, and internal eye diseases related to systemic diseases. The readers are medical workers and researchers related to clinical and basic research of fundus diseases. According to the journal retrieval report of the Chinese Institute of Scientific and Technological Information, the comprehensive ranking impact factor and total citation frequency of the Chinese Journal of Ocular Fundus Diseases have been among the best in the disciplines of ophthalmology, otolaryngology, and ophthalmology in my country for many years. The papers published have been included in many important databases at home and abroad, such as Scopus, Peking University Core, and China Science Citation Database (CSCD).