探讨鳄梨种子生产生物乙醇的潜力:沸水脱木质素预处理的研究

IF 2.4 Q3 ENERGY & FUELS
Herliati Rahman, Ayu Nehemia, Hadiatun Puji Astuti
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引用次数: 0

摘要

替代化石燃料的需求日益增加,使得生物乙醇成为一种很有前途的选择。尽管许多产糖和农业废物的来源可以转化为乙醇,但鳄梨种子(AS)作为原料特别有吸引力,因为它们丰富、碳水化合物含量高,并且缺乏与食物链的相互作用。因此,本研究通过几个步骤研究了AS在生物乙醇生产中的潜力,包括沸水脱木素预处理、催化水解和酿酒酵母发酵。AS的脱木素预处理包括在4%(w/v)氢氧化钠溶液中浸泡24小时。然后将混合物加热至80°C并缓慢搅拌2.5小时,然后用100°C的沸水洗涤1.5小时并筛选混合物。随后,使用两种不同浓度的酿酒酵母作为酵母进行催化水解和发酵,即10%(w/v)和15%(w/w)。使用扫描电子显微镜(SEM)进行定性样品分析以观察脱木素预处理的效果,而使用Thermo Scientific Nicolet iS50进行FTIR分析以测试葡萄糖官能团。使用气相色谱7890b质谱5977A、安捷伦DBVRX进行定量分析以测定水解产物发酵。结果表明,用15%(w/v)酵母和40%(v/v)催化剂发酵可获得最高的乙醇产量,乙醇产量为理论最大值的83.755%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Investigating the potential of avocado seeds for bioethanol production: A study on boiled water delignification pretreatment
The increasing need for alternative fuels to replace fossil fuels has made bioethanol a promising option. Although numerous sources of sugar generation and agricultural wastes can be converted into ethanol, Avocado Seeds (AS) are particularly attractive as raw materials due to their abundance, high carbohydrate content, and lack of interactions with the food chain. Therefore, this study investigated the potential of AS for bioethanol production using several steps, including boiled water delignification pretreatment, catalytic hydrolysis, and fermentation with Saccharomyces cerevisiae. The delignification pretreatment of AS involved soaking in 4% (w/v) sodium hydroxide liquor for 24 hours. Then the mixture was heated to 80°C and stirred slowly for 2.5 hours and after that washing with boiled water at 100 oC for 1.5 hours and screening the mixture. Subsequently, catalytic hydrolysis and fermentation were carried out using two different concentrations of Saccharomyces cerevisiae as yeast, namely 10% (w/v) and 15% (w/v). Qualitative sample analysis was conducted using scanning electron microscopy (SEM) to observe the effect of delignification pretreatment, while FTIR analysis using Thermo Scientific Nicolet iS50 was used to test for glucose functional groups. Quantitative analysis was performed using gas chromatography 7890b mass spectrophotometry 5977A, Agilent DBVRX to determine hydrolysate fermentation. The results revealed that the highest ethanol yield was achieved through fermentation with 15% (w/v) yeast and 40% (v/v) catalyst, resulting in an ethanol yield of 83.755% of the theoretical maximum.
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来源期刊
CiteScore
4.50
自引率
16.00%
发文量
83
审稿时长
8 weeks
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