液体细胞透射电子显微镜中介质电泳控制粒子组装的可行性

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
T. Yamazaki, Hiromasa Niinomi, Y. Kimura
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引用次数: 2

摘要

液池透射电子显微镜(LC-TEM)是一种有效的观察液体样品现象的技术,具有与传统透射电子显微镜(TEM)相似的空间和时间分辨率。因此,这种方法有望使以前传统光学显微镜无法实现的现象可视化。然而,由于难以控制观察区域内样品液体的状态,这种方法难以观察到成核等动态过程。为了解决这一问题,我们重点研究了用电极组装粒子的介电电泳,并利用相对比光学显微镜(PCM)和透射电镜(TEM)研究了将交流电流信号施加到现有液体电池中的电极上时发生的现象。在PCM中,我们观察到溶液中的胶体颗粒被吸引到电极上形成组件,颗粒沿着电场排列形成珍珠链,珍珠链积累形成胶体晶体。然而,由于相关支架设计的差异,在TEM研究中没有观察到这些现象。我们的研究结果表明,在LC-TEM中利用介电泳力进行粒子组装是可能的,但需要进一步的研究,包括电气设备的开发,才能在实践中实现这一点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Feasibility of control of particle assembly by dielectrophoresis in liquid-cell transmission electron microscopy
Abstract Liquid-cell transmission electron microscopy (LC-TEM) is a useful technique for observing phenomena in liquid samples with spatial and temporal resolutions similar to those of conventional transmission electron microscopy (TEM). This method is therefore expected to permit the visualization of phenomena previously inaccessible to conventional optical microscopy. However, dynamic processes such as nucleation are difficult to observe by this method because of difficulties in controlling the condition of the sample liquid in the observation area. To approach this problem, we focused on dielectrophoresis, in which electrodes are used to assemble particles, and we investigated the phenomena that occurred when an alternating-current signal was applied to an electrode in an existing liquid cell by using a phase-contrast optical microscope (PCM) and TEM. In PCM, we observed that colloidal particles in a solution were attracted to the electrodes to form assemblies, that the particles aligned along the electric field to form pearl chains and that the pearl chains accumulated to form colloidal crystals. However, these phenomena were not observed in the TEM study because of differences in the design of the relevant holders. The results of our study imply that the particle assembly by using dielectrophoretic forces in LC-TEM should be possible, but further studies, including electric device development, will be required to realize this in practice.
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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