新胶原诱导下的热损伤愈合:临床前随机实验研究

G. Mogilnaya, E. V. Fomicheva, K. Melkonian
{"title":"新胶原诱导下的热损伤愈合:临床前随机实验研究","authors":"G. Mogilnaya, E. V. Fomicheva, K. Melkonian","doi":"10.25207/1608-6228-2022-29-6-53-66","DOIUrl":null,"url":null,"abstract":"Background. The healing of skin wounds having various etiologies is known to involve a multistep process characterized by certain intercellular interactions affecting dermal cells, their attachment, migration, and differentiation. Here, recovery is interpreted as the return of dermis to its original state. The fact is, however, that the dermal extracellular matrix (ECM) is structurally impaired, which suppresses the regulatory and repository functions of the dermis, leading to the formation of a scar that inhibits several biological functions in the affected area and causes aesthetic problems associated with mobility.Objectives. To evaluate the structural features of dermis during wound healing using a calcium-containing biodegradable implant.Methods. The study used 60 rats that were inflicted with a third-degree burn injury (partially damaged dermis). The selected animals were divided into two groups: experimental and control. On post-burn day 14, a calcium-containing biodegradable implant was administered to rats from the experimental group, while a sterile saline solution was used in the control group. Material was sampled at two months (74 days) and four months (134 days). In order to assess the morphological state of the burn area, its sections were stained with hematoxylin and eosin, according to Mallory and Van-Gieson. For the selective detection of collagens, immunohistochemical tests using antibodies to collagen types I and III (Abcam, England) were employed. To characterize dermal cells, the authors used antibodies to vimentin (LabVision, USA), as well as to СD-68 (cluster of differentiation 68), α-SMA (alpha-smooth muscle actin), CD-105 (cluster of differentiation 105), and VEGF (vascular endothelial growth factor) receptors (Abcam, England). The obtained results were processed using the Statistica 6 software (StatSoft, USA).Results. The administration of a calcium-containing biodegradable filler during the burn healing process was found to ensure local fibroblast activation with the formation of collagen types I and III. When the implant residence time was prolonged up to four months, an increase in the number of macrophages expressing CD-68 receptors was observed. Of note is that these cells retained their localization, while α-SMA-expressing cells were localized in both the superficial and deep dermal compartments. The number of cells expressing CD-105 and VEGF rose as well.Conclusion. The use of the biodegradable filler is found to be promising in terms of post-burn dermal regeneration, as well as providing a dermal ECM, whose collagen network composition and assembly are similar to the original. Here, macrophages act as the primary synthesis regulators of the dermal ECM and stimulate fibroblasts, which ensures re-epithelialization and angiogenesis of the inflicted area.","PeriodicalId":33483,"journal":{"name":"Kubanskii nauchnyi meditsinskii vestnik","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Thermal injury healing in the context of neocollagenogenesis induction: preclinical randomized experimental study\",\"authors\":\"G. Mogilnaya, E. V. Fomicheva, K. Melkonian\",\"doi\":\"10.25207/1608-6228-2022-29-6-53-66\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background. The healing of skin wounds having various etiologies is known to involve a multistep process characterized by certain intercellular interactions affecting dermal cells, their attachment, migration, and differentiation. Here, recovery is interpreted as the return of dermis to its original state. The fact is, however, that the dermal extracellular matrix (ECM) is structurally impaired, which suppresses the regulatory and repository functions of the dermis, leading to the formation of a scar that inhibits several biological functions in the affected area and causes aesthetic problems associated with mobility.Objectives. To evaluate the structural features of dermis during wound healing using a calcium-containing biodegradable implant.Methods. The study used 60 rats that were inflicted with a third-degree burn injury (partially damaged dermis). The selected animals were divided into two groups: experimental and control. On post-burn day 14, a calcium-containing biodegradable implant was administered to rats from the experimental group, while a sterile saline solution was used in the control group. Material was sampled at two months (74 days) and four months (134 days). In order to assess the morphological state of the burn area, its sections were stained with hematoxylin and eosin, according to Mallory and Van-Gieson. For the selective detection of collagens, immunohistochemical tests using antibodies to collagen types I and III (Abcam, England) were employed. To characterize dermal cells, the authors used antibodies to vimentin (LabVision, USA), as well as to СD-68 (cluster of differentiation 68), α-SMA (alpha-smooth muscle actin), CD-105 (cluster of differentiation 105), and VEGF (vascular endothelial growth factor) receptors (Abcam, England). The obtained results were processed using the Statistica 6 software (StatSoft, USA).Results. The administration of a calcium-containing biodegradable filler during the burn healing process was found to ensure local fibroblast activation with the formation of collagen types I and III. When the implant residence time was prolonged up to four months, an increase in the number of macrophages expressing CD-68 receptors was observed. Of note is that these cells retained their localization, while α-SMA-expressing cells were localized in both the superficial and deep dermal compartments. The number of cells expressing CD-105 and VEGF rose as well.Conclusion. The use of the biodegradable filler is found to be promising in terms of post-burn dermal regeneration, as well as providing a dermal ECM, whose collagen network composition and assembly are similar to the original. Here, macrophages act as the primary synthesis regulators of the dermal ECM and stimulate fibroblasts, which ensures re-epithelialization and angiogenesis of the inflicted area.\",\"PeriodicalId\":33483,\"journal\":{\"name\":\"Kubanskii nauchnyi meditsinskii vestnik\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-12-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Kubanskii nauchnyi meditsinskii vestnik\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.25207/1608-6228-2022-29-6-53-66\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Kubanskii nauchnyi meditsinskii vestnik","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.25207/1608-6228-2022-29-6-53-66","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

背景。具有多种病因的皮肤伤口的愈合涉及一个多步骤的过程,其特征是某些影响真皮细胞的细胞间相互作用,它们的附着,迁移和分化。在这里,恢复被解释为真皮恢复到原来的状态。然而,事实是,真皮细胞外基质(ECM)在结构上受损,这抑制了真皮的调节和储存功能,导致疤痕的形成,抑制了受影响区域的几种生物功能,并导致与活动有关的美学问题。目的:探讨含钙生物可降解假体在伤口愈合过程中真皮的结构特征。这项研究使用了60只遭受三度烧伤(真皮部分受损)的大鼠。选取的动物分为两组:实验组和对照组。烧伤后第14天,实验组大鼠给予含钙生物可降解植入物,对照组大鼠给予无菌生理盐水。在2个月(74天)和4个月(134天)时取样。根据Mallory和Van-Gieson的说法,为了评估烧伤区域的形态状态,用苏木精和伊红对其切片进行了染色。对于胶原的选择性检测,采用I型和III型胶原抗体的免疫组织化学测试(Abcam, England)。为了表征真皮细胞,作者使用了针对vimentin(美国LabVision公司)的抗体,以及针对СD-68(分化簇68)、α-SMA (α-平滑肌肌动蛋白)、CD-105(分化簇105)和VEGF(血管内皮生长因子)受体(英国Abcam公司)的抗体。使用Statistica 6软件(StatSoft, USA)对所得结果进行处理。研究发现,在烧伤愈合过程中使用含钙的可生物降解填充物可确保局部成纤维细胞活化,形成I型和III型胶原。当植入物停留时间延长至4个月时,观察到表达CD-68受体的巨噬细胞数量增加。值得注意的是,这些细胞保留了它们的定位,而表达α- sma的细胞则定位于真皮浅层和深层。表达CD-105和VEGF的细胞数量也有所增加。生物可降解填料的使用在烧伤后真皮再生方面被发现是有前途的,并提供真皮ECM,其胶原网络组成和组装与原始相似。在这里,巨噬细胞作为真皮ECM的主要合成调节因子并刺激成纤维细胞,从而确保损伤区域的再上皮化和血管生成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Thermal injury healing in the context of neocollagenogenesis induction: preclinical randomized experimental study
Background. The healing of skin wounds having various etiologies is known to involve a multistep process characterized by certain intercellular interactions affecting dermal cells, their attachment, migration, and differentiation. Here, recovery is interpreted as the return of dermis to its original state. The fact is, however, that the dermal extracellular matrix (ECM) is structurally impaired, which suppresses the regulatory and repository functions of the dermis, leading to the formation of a scar that inhibits several biological functions in the affected area and causes aesthetic problems associated with mobility.Objectives. To evaluate the structural features of dermis during wound healing using a calcium-containing biodegradable implant.Methods. The study used 60 rats that were inflicted with a third-degree burn injury (partially damaged dermis). The selected animals were divided into two groups: experimental and control. On post-burn day 14, a calcium-containing biodegradable implant was administered to rats from the experimental group, while a sterile saline solution was used in the control group. Material was sampled at two months (74 days) and four months (134 days). In order to assess the morphological state of the burn area, its sections were stained with hematoxylin and eosin, according to Mallory and Van-Gieson. For the selective detection of collagens, immunohistochemical tests using antibodies to collagen types I and III (Abcam, England) were employed. To characterize dermal cells, the authors used antibodies to vimentin (LabVision, USA), as well as to СD-68 (cluster of differentiation 68), α-SMA (alpha-smooth muscle actin), CD-105 (cluster of differentiation 105), and VEGF (vascular endothelial growth factor) receptors (Abcam, England). The obtained results were processed using the Statistica 6 software (StatSoft, USA).Results. The administration of a calcium-containing biodegradable filler during the burn healing process was found to ensure local fibroblast activation with the formation of collagen types I and III. When the implant residence time was prolonged up to four months, an increase in the number of macrophages expressing CD-68 receptors was observed. Of note is that these cells retained their localization, while α-SMA-expressing cells were localized in both the superficial and deep dermal compartments. The number of cells expressing CD-105 and VEGF rose as well.Conclusion. The use of the biodegradable filler is found to be promising in terms of post-burn dermal regeneration, as well as providing a dermal ECM, whose collagen network composition and assembly are similar to the original. Here, macrophages act as the primary synthesis regulators of the dermal ECM and stimulate fibroblasts, which ensures re-epithelialization and angiogenesis of the inflicted area.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
0.10
自引率
0.00%
发文量
37
审稿时长
8 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信