金属有机骨架(MOF)整体柱的制备及高效液相色谱法测定中药中薯蓣皂苷的固相萃取

IF 1.3 4区工程技术 Q4 CHEMISTRY, ANALYTICAL

Instrumentation Science & Technology Pub Date : 2022-09-13 DOI:10.1080/10739149.2022.2120894

Sha Wang, Yamei Han, Jing Chen, Ligai Bai, Hongyuan Yan, Haiyan Liu

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引用次数: 0

摘要

摘要以改性MIL-101-NH2和甲基丙烯酸缩水甘油酯(GMA)为单体，采用原位聚合法制备了金属有机骨架(MOF)整体柱。采用整体柱作为固相萃取(SPE)吸附剂对中药薯蓣皂苷进行在线富集和初步纯化。通过扫描电镜(SEM)和氮气吸附-解吸等手段对其进行表征，发现添加MIL-101-NH2的整体柱的优势体现在孔结构和比表面积上。固相萃取整体柱在0.13µg/mL ~ 5.0 × 102µg/mL范围内呈线性关系。检测限(LOD)为0.04µg/mL，定量限(LOQ)为0.13µg/mL。回收率为96% ~ 104%，日内、日间相对标准偏差(rsd)均在1.1%以下。该方法适用于复杂样品中薯蓣皂苷的测定。

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Preparation of a metal organic framework (MOF)-based monolithic column for the solid-phase extraction (SPE) of diosmetin from traditional Chinese medicine with determination by high-performance liquid chromatography (HPLC)

Abstract A metal organic framework (MOF)-based monolithic column was prepared by in situ polymerization using modified MIL-101-NH2 and glycidyl methacrylate (GMA) as monomers. The monolithic column was used as a solid phase extraction (SPE) adsorbent for on-line enrichment and preliminary purification of diosmetin in traditional Chinese medicine. After being characterized by scanning electron microscopy (SEM) and nitrogen adsorption-desorption, the advantages of the monolithic column with MIL-101-NH2 added were reflected in the pore structure and specific surface area. The monolithic column for SPE had a linear relationship from 0.13 µg/mL to 5.0 × 102 µg/mL. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.04 µg/mL and 0.13 µg/mL, respectively. The recovery was from 96% to 104%, and the intra-day and inter-day relative standard deviations (RSDs) were below 1.1%. This method was suitable for the determination of diosmetin in complex samples.

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来源期刊

Instrumentation Science & Technology 工程技术-分析化学

CiteScore

3.50

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Instrumentation Science & Technology is an internationally acclaimed forum for fast publication of critical, peer reviewed manuscripts dealing with innovative instrument design and applications in chemistry, physics biotechnology and environmental science. Particular attention is given to state-of-the-art developments and their rapid communication to the scientific community. Emphasis is on modern instrumental concepts, though not exclusively, including detectors, sensors, data acquisition and processing, instrument control, chromatography, electrochemistry, spectroscopy of all types, electrophoresis, radiometry, relaxation methods, thermal analysis, physical property measurements, surface physics, membrane technology, microcomputer design, chip-based processes, and more. Readership includes everyone who uses instrumental techniques to conduct their research and development. They are chemists (organic, inorganic, physical, analytical, nuclear, quality control) biochemists, biotechnologists, engineers, and physicists in all of the instrumental disciplines mentioned above, in both the laboratory and chemical production environments. The journal is an important resource of instrument design and applications data.