口服丙烯酰胺对Wistar大鼠胃肠运动和肠道结构的影响

A. Ige, O. Ayoola, E. Oladejo, B. Adele, O. Ola, E. Adewoye
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引用次数: 2

摘要

简介:丙烯酰胺是烹饪过程中的一种副产品,据报道是一种有毒物质,可能具有致癌特性。它对胃功能的损害以前已有报道。在本研究中,研究了其对雄性Wistar大鼠胃肠运动和肠道结构的影响。方法:将45只大鼠(120-180g)分为3组(n=15),用0.2ml蒸馏水或丙烯酰胺(分别为7.5mg/kg和15mg/kg)处理28天。之后,评估胃排空和肠动力。使用组织学技术评估肠道结构(十二指肠、空肠和回肠)、粘膜和肠道细胞计数。结果:与对照组相比,实验组(丙烯酰胺处理组;7.5mg/kg和15mg/kg)的胃排空和肠道转运时间增加(p<0.05)。与对照组相比,实验组的粘膜细胞计数(十二指肠、空肠和回肠)和回肠肠细胞计数(p<0.05)减少。与对照组相比,实验组的十二指肠样本显示出严重的凝固性坏死和绒毛脱落,管腔充满坏死碎片,李隐窝破裂和坏死,中度多形核细胞浸润和血管充血。在丙烯酰胺处理组的空肠和回肠中也观察到这些病理,尽管严重程度较低。结论:增加丙烯酰胺的口腔暴露会损害胃排空、肠道运动、粘液分泌,并损害小肠的消化和吸收功能,尤其是十二指肠。这些观察结果可归因于丙烯酰胺诱导的神经元信号受损、自主神经病变、氧化应激、炎症和细胞坏死。关键词:丙烯酰胺,胃肠道,胃排空,肠动力,小肠
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Gastrointestinal motility and Intestinal structure following oral exposure to acrylamide in Wistar rats
Introduction: Acrylamide, a byproduct of the cooking process, has been reported to be a toxicant with likely carcinogenic properties. Its impairment of gastric function has been previously reported. In this study its effects on gastrointestinal motility and intestinal structure was investigated in male Wistar rats.Methods: Forty-five rats (120-180g) were divided into 3 equal groups (n=15) and treated p.o with either 0.2ml distilled-water, or acrylamide (7.5mg/kg and 15mg/kg respectively) for 28days. Thereafter, gastric emptying and intestinal motility was assessed. Intestinal structure (duodenum, jejunum and ileum), mucosal and intestinal cell counts were evaluated using histological techniques.Results: Gastric emptying and intestinal transit time increased (p<0.05) in the experimental (acrylamidetreated; 7.5mg/kg and 15mg/kg) groups compared to control. Mucosal cell counts (duodenum, jejunum and ileum) and ileum intestinal cell counts (p<0.05) were reduced in the experimental groups compared to control. Compared to control, duodenal samples of the experimental groups showed severe coagulative necrosis and sloughing off of the villi, luminal filling with necrotic debris, disruption and necrosis of the crypts of Lieberkühn, moderate polymorphonuclear cell infiltration and vascular congestion. These pathologies albeit with less severity were also observed in the jejunum and ileum of acrylamide treated groups.Conclusion: Increased oral exposure to acrylamide impairs gastric emptying, intestinal motility, mucus secretion and compromises digestive and absorptive functions of the small intestines, especially the duodenum. These observations may be ascribed to acrylamide-induced impaired neuronal signaling, autonomic neuropathy, oxidative stress, inflammation and cell necrosis. Keywords: Acrylamide, gastrointestinal tract, gastric emptying, intestinal motility, small intestines
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