{"title":"QuEChERS-石墨烯-UPLC法测定牛肉中磷酸腺苷","authors":"Xiaoyan Duan","doi":"10.21162/pakjas/22.140","DOIUrl":null,"url":null,"abstract":"A rapid and highly sensitive modified QuEChERS method was combined with ultrahigh performance liquid chromatography (UPLC) to determine the content of adenosine phosphate in beef sample. In view of the complexity of the beef sample matrix, the cold perchloric acid was used as the solvent combined with vortex oscillation were selected to extracted the adenosine phosphate in the beef sample. 10mg graphene was used to remove interferences such as fat and pigment in beef, and UPLC was used for quantitative analysis. The three adenosine phosphates in beef showed a good linear relationship in the concentration range of 0.5-20 μg/mL, the linear correlation coefficient R2>0.99, and the average recovery rate of the three adenosine phosphate addition levels in beef samples was between 85.25%-100.12%, and the RSD was ranged from 1.63% to 3.69%. The adenosine phosphate content in commercially available beef samples further verifies the feasibility of this method. The proposed method has the advantages of simple and fast operation, good purification effect, high sensitivity and accuracy, therefor, may provide a new method for efficient and accurate evaluation of the quality and safety of beef","PeriodicalId":19885,"journal":{"name":"Pakistan Journal of Agricultural Sciences","volume":null,"pages":null},"PeriodicalIF":0.7000,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Determination of Adenosine phosphate in beef by QuEChERS- Graphene -UPLC\",\"authors\":\"Xiaoyan Duan\",\"doi\":\"10.21162/pakjas/22.140\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A rapid and highly sensitive modified QuEChERS method was combined with ultrahigh performance liquid chromatography (UPLC) to determine the content of adenosine phosphate in beef sample. In view of the complexity of the beef sample matrix, the cold perchloric acid was used as the solvent combined with vortex oscillation were selected to extracted the adenosine phosphate in the beef sample. 10mg graphene was used to remove interferences such as fat and pigment in beef, and UPLC was used for quantitative analysis. The three adenosine phosphates in beef showed a good linear relationship in the concentration range of 0.5-20 μg/mL, the linear correlation coefficient R2>0.99, and the average recovery rate of the three adenosine phosphate addition levels in beef samples was between 85.25%-100.12%, and the RSD was ranged from 1.63% to 3.69%. The adenosine phosphate content in commercially available beef samples further verifies the feasibility of this method. The proposed method has the advantages of simple and fast operation, good purification effect, high sensitivity and accuracy, therefor, may provide a new method for efficient and accurate evaluation of the quality and safety of beef\",\"PeriodicalId\":19885,\"journal\":{\"name\":\"Pakistan Journal of Agricultural Sciences\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.7000,\"publicationDate\":\"2022-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pakistan Journal of Agricultural Sciences\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.21162/pakjas/22.140\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pakistan Journal of Agricultural Sciences","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.21162/pakjas/22.140","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
Determination of Adenosine phosphate in beef by QuEChERS- Graphene -UPLC
A rapid and highly sensitive modified QuEChERS method was combined with ultrahigh performance liquid chromatography (UPLC) to determine the content of adenosine phosphate in beef sample. In view of the complexity of the beef sample matrix, the cold perchloric acid was used as the solvent combined with vortex oscillation were selected to extracted the adenosine phosphate in the beef sample. 10mg graphene was used to remove interferences such as fat and pigment in beef, and UPLC was used for quantitative analysis. The three adenosine phosphates in beef showed a good linear relationship in the concentration range of 0.5-20 μg/mL, the linear correlation coefficient R2>0.99, and the average recovery rate of the three adenosine phosphate addition levels in beef samples was between 85.25%-100.12%, and the RSD was ranged from 1.63% to 3.69%. The adenosine phosphate content in commercially available beef samples further verifies the feasibility of this method. The proposed method has the advantages of simple and fast operation, good purification effect, high sensitivity and accuracy, therefor, may provide a new method for efficient and accurate evaluation of the quality and safety of beef
期刊介绍:
Pakistan Journal of Agricultural Sciences is published in English four times a year. The journal publishes original articles on all aspects of agriculture and allied fields.