小麦穗部相关性状和株高数量性状位点的分子定位与验证

作物学报 Pub Date : 2022-06-01 DOI:10.3724/sp.j.1006.2022.11055

Wenjing Hu, Dong-sheng Li, Xin Yi, Chunguang Zhang, Yong Zhang

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引用次数: 1

摘要

穗相关性状和株高是小麦育种的重要目标性状。本研究从CIMMYT小麦品系C615和扬麦13（YM13）的杂交中构建了198个重组自交系（RIL）群体，然后用小麦90K SNP阵列进行基因分型，并在三种环境中对穗相关性状和株高进行表型分型，以挖掘这些性状的QTL（数量性状位点）。利用复合区间作图法，我们鉴定了一个每穗总小穗数（TSS）QTL、两个穗长（SL）QTL，两个小穗紧凑度（SC）QTL和三个株高（PH）QTL。QSN.yaas-3B和QPH.yaas-3B在3B染色体上重叠。QSL.yaas-5A、QSC.yaas-5B和QPH.yaas-5C在5A染色体上重叠。QSL.yaas-6A和QSC.yaas-6A。QSN.yaas-3B/QPH.yaas-3B和QSL.yaas-6A/QSC.yaas-6A尚未报道，可能是新的基因座。将与QSL.yaas-5A/QSC.yaas-5A/QPH.yaas-5A紧密连接的SNP标记转化为一个竞争性等位基因特异性PCR（KASP）标记（QC615-5A-KASP），并在105个小麦品系中进行验证。这一结果将有助于小麦育种中产量相关性状的改良。

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Molecular mapping and validation of quantitative trait loci for spike-related traits and plant height in wheat

Spike-related traits and plant height are important target traits in wheat breeding. In the present study, a population of 198 recombinant inbred lines (RILs) derived from the cross between a CIMMYT wheat line C615 and Yangmai 13 (YM13) was constructed, followed by genotyping with Wheat 90K SNP array and phenotyping of spike-related traits and plant height in three environments to excavate QTLs (quantitative trait loci) for these traits. Using composite interval mapping method, we identified one QTL for total spikelet number per spike (TSS), two QTLs for spike length (SL), two QTLs for spikelet compactness (SC), and three QTLs for plant height (PH). QSN.yaas-3B and QPH.yaas-3B overlapped on the chromosome 3B. QSL.yaas-5A, QSC.yaas-5A and QPH.yaas-5A overlapped on the chromosome 5A. QSL.yaas-6A and QSC.yaas-6A overlapped on the chromosome 6A. QSN.yaas-3B/QPH.yaas-3B and QSL.yaas-6A/QSC.yaas-6A had not been reported yet, and were likely to be novel loci. The SNP marker closely linked to QSL.yaas-5A/QSC.yaas-5A/QPH.yaas-5A was then converted into one Kompetitive Allele Specific PCR (KASP) marker (QC615-5A-KASP), and validated in a panel of 105 wheat lines. The results would be useful for improvement of yield related traits in wheat breeding.

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来源期刊

作物学报 Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

1.70

自引率

0.00%

发文量

期刊介绍： The major aims of AAS are to report the progresses in the disciplines of crop breeding, crop genetics, crop cultivation, crop physiology, ecology, biochemistry, germplasm resources, grain chemistry, grain storage and processing, bio-technology and biomathematics etc. mainly in China and abroad. AAS provides regular columns for Original papers, Reviews, and Research notes. The strict peer-review procedure guarantees the academic level and raises the reputation of the journal. The readership of AAS is for crop science researchers, students of agricultural colleges and universities, and persons with similar academic level.