几内亚共和国孕妇群体中的艾滋病毒:频率和遗传特征

Q4 Medicine
T. Balde, A. Shchemelev, Y. Ostankova, S. Boumbaly, D. Valutite, V. S. Davydenko, E. Serikova, E. B. Zueva, E. Anufrieva, V. V. Skvoroda, D. A. Vasileva, E. Esaulenko, A. Semenov, A. Totolian
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引用次数: 0

摘要

本研究的目的是评估几内亚共和国孕妇人体免疫缺陷病毒的流行率和分子遗传特征研究。材料和方法。这项研究的材料是来自几内亚共和国的972名孕妇的血浆样本。检查患者是否存在HIV感染血清学(Ag+Ab)和分子标记物(RNA)。对于PCR结果呈阳性且病毒载量充足(>500c/ml)的患者,通过Sanger测序获得负责合成pro和rev蛋白的pol基因片段的遗传序列。这些序列用于系统发育分析,并检测耐药性突变。结果和讨论。ELISA阳性率为12.96%。在ELISA阳性的女性中,76.98%的病例检测到RNA,但在11例病例中,在没有HIV感染血清学标志物的患者中检测到RNA。因此,在整个调查人群中,HIV RNA的发生率为11.11%。在绝大多数病例中,发现了循环重组形式02_AG。根据分析,我们认为重组形式的艾滋病毒对研究区域病毒的遗传多样性有重大贡献。DR突变的发生率很高(26.80%)。最常见的取代发生在蛋白酶的20位(70.10%,95%CI 59.96–78.98%),其中K20I突变占主导地位。此外,L10I/V突变相对常见,增加了具有其他PI抗性突变的病毒的复制。在与HIV对NNRTI的耐药性相关的突变中,发现了一个非多态性突变V179T。结论本研究中确定的影响预防母婴传播有效性的一个重要因素是几内亚孕妇PDR的高患病率。这项研究在孕妇和未接受抗逆转录病毒疗法的妇女中发现的耐药性突变的高流行率表明,几内亚目前的治疗方案不足以预防艾滋病毒的垂直感染。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
HIV in pregnant women group in the Republic of Guinea: frequency and genetic characteristics
The aim of this study was to assess the prevalence and study of the molecular genetic characteristics of the human immunodeficiency virus in pregnant women of the Republic of Guinea.Materials and methods. The material for the study was blood plasma samples of 972 pregnant women from the Republic of Guinea. The patients were examined for the presence of HIV infection serological (Ag+Ab) and molecular markers (RNA). For patients with a positive PCR result and a sufficient viral load (>500 c/ml), the genetic sequences of the pol gene fragment responsible for the synthesis of pro and rev proteins were obtained by Sanger sequencing. These sequences were used for phylogenetic analysis and examined for drug resistance mutations.Results and discussion. 12.96% of patients was positive in ELISA. Among women who were positive in ELISA, RNA was detected in 76.98% of cases, however, in 11 cases, RNA was detected in patients without serological markers of HIV infection, so the incidence of HIV RNA in the entire surveyed population was 11.11%. In the vast majority of cases, the circulating recombinant form 02_AG is found. Based on the analysis, we assume a significant contribution of recombinant forms of HIV to the genetic diversity of the virus in the region under study.The incidence of DR mutations was quite high (26.80%). The most frequent substitutions were in position 20 of the protease (70.10%, 95% CI 59.96–78.98%), of which the K20I mutation was dominant. In addition, the L10I/V mutation was relatively common, increasing the replication of viruses with other PI resistance mutations. Among the mutations associated with HIV resistance to NNRTIs, a non-polymorphic mutation V179T was found.Conclusion. An important factor influencing the effectiveness of Prevention of Mother to Child Transmission identified in this study was the high prevalence of PDR among pregnant women in Guinea. The high prevalence of drug resistance mutations found in this study in pregnant women, as well as in ART-naive women, indicates that current regimens in Guinea are insufficient to prevent vertical HIV infection.
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来源期刊
HIV Infection and Immunosuppressive Disorders
HIV Infection and Immunosuppressive Disorders Medicine-Infectious Diseases
CiteScore
0.70
自引率
0.00%
发文量
37
期刊介绍: In the scientific-practical journal "HIV Infection and Immunosuppressive Disorders", published various issues of HIV medicine (epidemiology, molecular mechanisms of pathogenesis to the development of educational programs) leading scientists of Russia and countries of CIS, USA, as well as practical healthcare professionals working in research centers, research institutes, universities, clinics where done basic medical work. A special place on the pages of the publication is given to basic and clinical research, analytical reviews of contemporary and foreign reports, the provision of medical care for various diseases.
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