胼胝体金纳米颗粒对多发性硬化症模型髓磷脂的增强作用

IF 1.4 Q4 NANOSCIENCE & NANOTECHNOLOGY
M. Khosravi, M. Karami, M. J. Nadoushan, A. Hajnorouzi
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引用次数: 1

摘要

目的:在不需要大量费用的情况下,我们将L-精氨酸注射到大鼠胼胝体(CC)中以建立多发性硬化(MS)动物模型,并研究了金纳米粒子(GNPs)的注射前效应。材料和方法:成年雄性Wistar大鼠(250-300g)通过手术在CC处插管,恢复后每天注射L-精氨酸(3-200µg/只,CC内)一次,连续3-5天。GNP(0.001-0.01µg/大鼠,CC内)单独注射或在使用相同程序注射L-精氨酸之前注射。对照组仅接受生理盐水(1µL/大鼠,CC内)。用luxol快蓝对大脑进行了研究。体重变化也通过方差分析(ANOVA)进行分析。结果:L-精氨酸可显著降低(p<0.05)纤维密度,而神经元数量增加(p<0.01),单次GNP可降低(p>0.05)纤维和神经元密度;然而,NPs的预注射引起有髓鞘纤维和神经元的均匀密度。结论:L-精氨酸可能通过促炎性一氧化氮(NO)触发脱髓鞘,GNPs可能改善这种作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Myelin enhancement of Multiple sclerosis model with gold nanoparticles into the corpus callosum
Objective(s): With no substantial cost, we injected L-arginine into the rat’s corpus callosum (CC) to create animal model of multiple sclerosis (MS) and investigated the pre-injection effect of gold nanoparticles (GNPs). Materials and Methods: Adult male Wistar rat (250-300 g) was surgically cannulated at the CC, and after recovery it was injected L-arginine (3-200 µg/rat, intra-CC) once daily for 3 to 5 consecutive days. GNPs (0.001-0.01 µg/rat, intra-CC) were injected alone or prior to the L-arginine using the same procedure. Control group solely received saline (1 µL/rat, intra-CC). Brain was studied with luxol fast blue. Weight change was also analyzed via the analysis of variance (ANOVA). Results: L-arginine significantly induced (p< 0.05) a reduction in the fiber density while the neurons increased (p< 0.05). Single GNPs reduced (p< 0.05) the fiber and neuron densities; however, pre-injection of NPs caused myelinated fibers and uniform density of neurons. Conclusion: The L-arginine may trigger demyelination by pro-inflammatory nitric oxide (NO), and the GNPs may improve this effect.
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来源期刊
Nanomedicine Journal
Nanomedicine Journal NANOSCIENCE & NANOTECHNOLOGY-
CiteScore
3.40
自引率
0.00%
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0
审稿时长
12 weeks
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