IDH野生型胶质母细胞瘤中EGFL7的表达谱与不良患者预后相关

IF 1.7 Q3 PATHOLOGY

Journal of Pathology and Translational Medicine Pub Date : 2022-06-15 DOI:10.4132/jptm.2022.04.22

Bruno Henrique Bressan da Costa, A. Becker, L. Neder, P. G. Gonçalves, Cristiane Carla de Oliveira, A. Polverini, C. Clara, G. Teixeira, R. Reis, L. Bidinotto

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The genes strongly correlated to EGFL7 expression were submitted to enrichment gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Additionally, EGFL7 expression was associated with patient overall survival. The expression of EGFL7 was analyzed through immunohistochemistry in 74 GBM IDH-wildtype patients’ samples, and was associated with clinicopathological data and overall survival. Results In silico analysis found 78 genes strongly correlated to EGFL7 expression. These genes were enriched in 40 biological processes and eight KEGG pathways, including angiogenesis/vasculogenesis, cell adhesion, and phosphoinositide 3-kinase–Akt, Notch, and Rap1 signaling pathways. The immunostaining showed high EGFL7 expression in 39 cases (52.7%). High immunolabelling was significantly associated with low Karnofsky Performance Status and poor overall survival. Cox analysis showed that GBMs IDH-wildtype with high EGFL7 expression presented a higher risk of death compared to low expression (hazard ratio, 1.645; 95% confidence interval, 1.021 to 2.650; p = .041). Conclusions This study gives insights regarding the genes that are correlated with EGFL7, as well as biological processes and signaling pathways, which should be further investigated in order to elucidate their role in glioblastoma biology.","PeriodicalId":46933,"journal":{"name":"Journal of Pathology and Translational Medicine","volume":"56 1","pages":"205 - 211"},"PeriodicalIF":1.7000,"publicationDate":"2022-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"EGFL7 expression profile in IDH-wildtype glioblastomas is associated with poor patient outcome\",\"authors\":\"Bruno Henrique Bressan da Costa, A. Becker, L. Neder, P. G. Gonçalves, Cristiane Carla de Oliveira, A. Polverini, C. 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引用次数: 2

摘要

背景尽管胶质母细胞瘤（GBM）的治疗取得了进展，但患者的平均寿命为14个月。因此，迫切需要确定预后、治疗反应或开发新的治疗策略的生物标志物。我们先前描述了高表皮生长因子样结构域多重7（EGFL7）表达与毛细胞星形细胞瘤患者不良预后的关系。本研究旨在使用免疫组织化学和计算机方法分析EGFL7在GBM异柠檬酸脱氢酶（IDH）-野生型中的预后潜力。方法对癌症基因组图谱RNA测序数据进行Spearman相关性分析。将与EGFL7表达密切相关的基因提交给富集基因本体论和京都基因和基因组百科全书（KEGG）分析。此外，EGFL7的表达与患者的总生存率相关。通过免疫组化分析了74例GBM IDH野生型患者样本中EGFL7的表达，并与临床病理数据和总生存率相关。结果计算机分析发现78个基因与EGFL7的表达密切相关。这些基因在40个生物学过程和8个KEGG途径中富集，包括血管生成/血管生成、细胞粘附和磷酸肌醇3-激酶-Akt、Notch和Rap1信号通路。免疫染色显示39例（52.7%）EGFL7高表达。高免疫标记与低Karnofsky性能状态和低总生存率显著相关。Cox分析显示，与低表达相比，EGFL7高表达的GBMs-IDH野生型具有更高的死亡风险（风险比，1.645；95%置信区间，1.021-2.650；p=0.041），为了阐明它们在胶质母细胞瘤生物学中的作用，应该对其进行进一步的研究。

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EGFL7 expression profile in IDH-wildtype glioblastomas is associated with poor patient outcome

Background Despite the advances in glioblastoma (GBM) treatment, the average life span of patients is 14 months. Therefore, it is urgent to identity biomarkers of prognosis, treatment response, or development of novel treatment strategies. We previously described the association of high epidermal growth factor-like domain multiple 7 (EGFL7) expression and unfavorable outcome of pilocytic astrocytoma patients. The present study aims to analyze the prognostic potential of EGFL7 in GBM isocitrate dehydrogenase (IDH)-wildtype, using immunohistochemistry and in silico approaches. Methods Spearman’s correlation analysis of The Cancer Genome Atlas RNA sequencing data was performed. The genes strongly correlated to EGFL7 expression were submitted to enrichment gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Additionally, EGFL7 expression was associated with patient overall survival. The expression of EGFL7 was analyzed through immunohistochemistry in 74 GBM IDH-wildtype patients’ samples, and was associated with clinicopathological data and overall survival. Results In silico analysis found 78 genes strongly correlated to EGFL7 expression. These genes were enriched in 40 biological processes and eight KEGG pathways, including angiogenesis/vasculogenesis, cell adhesion, and phosphoinositide 3-kinase–Akt, Notch, and Rap1 signaling pathways. The immunostaining showed high EGFL7 expression in 39 cases (52.7%). High immunolabelling was significantly associated with low Karnofsky Performance Status and poor overall survival. Cox analysis showed that GBMs IDH-wildtype with high EGFL7 expression presented a higher risk of death compared to low expression (hazard ratio, 1.645; 95% confidence interval, 1.021 to 2.650; p = .041). Conclusions This study gives insights regarding the genes that are correlated with EGFL7, as well as biological processes and signaling pathways, which should be further investigated in order to elucidate their role in glioblastoma biology.

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来源期刊

Journal of Pathology and Translational Medicine PATHOLOGY-

CiteScore

5.00

自引率

4.20%

发文量

审稿时长

14 weeks

期刊介绍： The Journal of Pathology and Translational Medicine is an open venue for the rapid publication of major achievements in various fields of pathology, cytopathology, and biomedical and translational research. The Journal aims to share new insights into the molecular and cellular mechanisms of human diseases and to report major advances in both experimental and clinical medicine, with a particular emphasis on translational research. The investigations of human cells and tissues using high-dimensional biology techniques such as genomics and proteomics will be given a high priority. Articles on stem cell biology are also welcome. The categories of manuscript include original articles, review and perspective articles, case studies, brief case reports, and letters to the editor.