印度海产品加工厂食品接触表面分离大肠杆菌的流行、耐药性和分子特征

Q3 Agricultural and Biological Sciences

Journal of Food Quality and Hazards Control Pub Date : 2022-06-15 DOI:10.18502/jfqhc.9.1.9685

B. Francis, A. Antony, D. P. Sukumaran, A. Hatha

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引用次数: 0

摘要

背景:病原菌在生物膜中的生存对食品安全构成威胁。本研究的目的是确定流行，抗菌素耐药性和大肠杆菌菌株的分子特性。方法:对印度海产品前加工厂食品接触表面形成的生物膜进行拭子取样(n=144)。采用聚合酶链式反应(PCR)对大肠杆菌进行了分离鉴定。根据菌株的耐药性和毒力基因的存在进行了风险评估。采用Kirby-Bauer纸片扩散法进行药敏试验。采用四重PCR进行系统发育分组。采用肠杆菌重复基因间一致性pcr (ERIC-PCR)对菌株进行分子分型。数据采用SPSS 22进行统计分析。结果:肠致病性大肠杆菌(EPEC)是最常见的血清型。多重PCR分析显示存在志贺毒素基因(stx1, stx2)，内膜素(eae)和肠溶血素基因(hlyA)。志贺毒素基因stx2的检出率最高(83.33%)。各类群中以B1(45.56%)和B2(30%)居多。大肠杆菌对氨苄西林(85.56%)、哌拉西林(84.44%)和头孢多肟(85.56%)的耐药率普遍较高。结论:存在遗传异质性的多药耐药大肠杆菌菌株，具有潜在的毒力，在海产品行业具有较高的危险性。

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Prevalence, Antimicrobial Resistance, and Molecular Characterization of Escherichia coli Isolated from Food Contact Surfaces in Seafood Pre-Processing Plants (India)

Background: The survival of pathogens in biofilms poses a threat to food safety. The aim of this study was to determine prevalence, antimicrobial resistance, and molecular characterization of Escherichia coli strains. Methods: Swab samples (n=144) were collected from biofilm formed on food contact surfaces in seafood pre-processing plant in India. E. coli was isolated and identified using uid A gene by Polymerase Chain Reaction (PCR). The risk assessment of the isolates was carried out in terms of their drug resistance and the presence of virulence genes. Antimicrobial susceptibility testing was performed using the Kirby-Bauer disk diffusion method. Phylogenetic grouping was done by quadruplex PCR. Molecular typing of the strains was performed by Enterobacterial Repetitive Intergenic Consensus-PCR (ERIC-PCR). Data were statistically analyzed using SPSS version 22. Results: Enteropathogenic E. coli (EPEC) strains were the most prevalent serotype. Multiplex PCR analysis revealed the presence of shiga toxin genes (stx1, stx2), intimin (eae), and enterohemolysin genes (hlyA). Shiga toxin gene stx2 showed the highest prevalence (83.33%). Among various phylogroups, B1 (45.56%) and B2 (30%) were the most prevalent phylogroups. Resistance to ampicillin (85.56%), piperacillin (84.44%), and cefpodoxime (85.56%) was widespread among the E. coli strains. Conclusion: The presence of genetically heterogeneous multi drug resistant E. coli strains with virulence potential showed a high risk in the seafood industry.

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来源期刊

Journal of Food Quality and Hazards Control Agricultural and Biological Sciences-Food Science

CiteScore

1.50

自引率

0.00%

发文量

审稿时长

8 weeks

期刊介绍： Journal of Food Quality and Hazards Control (J. Food Qual. Hazards Control) is an international peer-reviewed quarterly journal that aims at publishing of high quality articles involved in food quality, food hygiene, food safety, and food control which scientists from all over the world may submit their manuscript. This academic journal aims to improve international exchange of new findings and recent developments in all aspects of agricultural and biological sciences. This free of charge journal is published in both online and print forms and welcomes the manuscripts that fulfill the general criteria of novelty and scientific importance. Among the most significant objectives of Journal of Food Quality and Hazards Control are to ensure that the articles reflect a wide range of topics regarding journal scopes; to do a fair, scientific, fast, as well as high quality peer-review process; to provide a wide and diverse geographical coverage of articles around the world; and to publish the articles having a trustable resource of scientific information for the audiences. The types of acceptable submissions include original article, review article, short communication, letter to the editor, case report, editorial, as well as book review. Journal of Food Quality and Hazards Control is an official journal of Research Center for Food Hygiene and Safety, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.