Daniela Muñiz-Anguiano, Manuel Gerardo Verduzco-Zapata, Marco A. Liñán-Cabello
{"title":"墨西哥太平洋海岸恢复过程中Pocillopora spp. (Anthozoa:菌核)反应的相关因素","authors":"Daniela Muñiz-Anguiano, Manuel Gerardo Verduzco-Zapata, Marco A. Liñán-Cabello","doi":"10.4067/S0718-19572017000200009","DOIUrl":null,"url":null,"abstract":"espanolDurante un proceso de restauracion de Pocillopora spp., usando corales de oportunidad (CO), fueron evaluados diversos atributos bioticos en dos sitios a lo largo de un ano, de manera bimensual fueron determinadas las concentraciones de aminoacidos tipo micosporina (AAMs) y niveles de clorofila a (Cl a). De manera especifica, en el ultimo muestreo se realizo un analisis de malonaldehido (MDA) y se calcularon las relaciones ARN/ADN y proteina/ADN como indicadores de estres oxidativo y actividad trascripcional en simbionte y cnidario de todos los morfotipos. En sitio Carrizales (SC) la tecnica de restauracion de ramilletes coralinos (RC) promovio la integracion de COs registrando signos de recuperacion y altas tasas de supervivencia (90%); no obstante, en el Sitio Juluapan (SJ), se observo una falta de respuesta a los indicadores bioticos y moleculares asociados a la recuperacion. Por lo tanto, los CO provenientes de Carrizales no fueron capaces de aclimatarse a un sitio en el que imperan distintas condiciones ambientales. Altas concentraciones de Cl a, AAMs, y una alta relacion ARN/ADN fueron caracteristicas de una condicion saludable de corales, que a su vez, estuvieron asociados a los indicadores de restauracion y mejor supervivencia observada. EnglishDuring a restoration process of Pocillopora spp., using opportunity corals (CO) several biotic attributes were assessed at two sites bi-monthly over a year and concentrations of mycosporin type amino acids (MAAs) and levels of chlorophyll a (Chl a) were determined. Specifically, in the last sampling analysis it was performed an analysis of malonaldehyde (MDA) and the RNA/DNA and protein/DNA relationships were calculated, as an index of oxidative stress and transcriptional activity in symbiont and coral tissue of all morphotypes. At Carrizales site (SC) the coral clusters (RC) used as a method of restoration, promoted the integration of CO showing signs of recovery and high survival rates (90%); however, in Juluapan site (SJ), a lack of response to biotic and molecular indicators associated with recovery was observed. Therefore, CO from Carrizales were not able to acclimate to a place where different conditions prevail. High concentrations of Chl a, AAMs and a high RNA/DNA ratio were characteristic of a healthy condition of coral which in turn, were associated with higher specimen's survival and restoration.","PeriodicalId":0,"journal":{"name":"","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4067/S0718-19572017000200009","citationCount":"3","resultStr":"{\"title\":\"Factores asociados a la respuesta de Pocillopora spp. (Anthozoa: Scleractinia) durante un proceso de restauración en la costa del Pacífico mexicano\",\"authors\":\"Daniela Muñiz-Anguiano, Manuel Gerardo Verduzco-Zapata, Marco A. Liñán-Cabello\",\"doi\":\"10.4067/S0718-19572017000200009\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"espanolDurante un proceso de restauracion de Pocillopora spp., usando corales de oportunidad (CO), fueron evaluados diversos atributos bioticos en dos sitios a lo largo de un ano, de manera bimensual fueron determinadas las concentraciones de aminoacidos tipo micosporina (AAMs) y niveles de clorofila a (Cl a). De manera especifica, en el ultimo muestreo se realizo un analisis de malonaldehido (MDA) y se calcularon las relaciones ARN/ADN y proteina/ADN como indicadores de estres oxidativo y actividad trascripcional en simbionte y cnidario de todos los morfotipos. En sitio Carrizales (SC) la tecnica de restauracion de ramilletes coralinos (RC) promovio la integracion de COs registrando signos de recuperacion y altas tasas de supervivencia (90%); no obstante, en el Sitio Juluapan (SJ), se observo una falta de respuesta a los indicadores bioticos y moleculares asociados a la recuperacion. Por lo tanto, los CO provenientes de Carrizales no fueron capaces de aclimatarse a un sitio en el que imperan distintas condiciones ambientales. Altas concentraciones de Cl a, AAMs, y una alta relacion ARN/ADN fueron caracteristicas de una condicion saludable de corales, que a su vez, estuvieron asociados a los indicadores de restauracion y mejor supervivencia observada. EnglishDuring a restoration process of Pocillopora spp., using opportunity corals (CO) several biotic attributes were assessed at two sites bi-monthly over a year and concentrations of mycosporin type amino acids (MAAs) and levels of chlorophyll a (Chl a) were determined. Specifically, in the last sampling analysis it was performed an analysis of malonaldehyde (MDA) and the RNA/DNA and protein/DNA relationships were calculated, as an index of oxidative stress and transcriptional activity in symbiont and coral tissue of all morphotypes. At Carrizales site (SC) the coral clusters (RC) used as a method of restoration, promoted the integration of CO showing signs of recovery and high survival rates (90%); however, in Juluapan site (SJ), a lack of response to biotic and molecular indicators associated with recovery was observed. Therefore, CO from Carrizales were not able to acclimate to a place where different conditions prevail. High concentrations of Chl a, AAMs and a high RNA/DNA ratio were characteristic of a healthy condition of coral which in turn, were associated with higher specimen's survival and restoration.\",\"PeriodicalId\":0,\"journal\":{\"name\":\"\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0,\"publicationDate\":\"2017-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.4067/S0718-19572017000200009\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.4067/S0718-19572017000200009\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.4067/S0718-19572017000200009","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Factores asociados a la respuesta de Pocillopora spp. (Anthozoa: Scleractinia) durante un proceso de restauración en la costa del Pacífico mexicano
espanolDurante un proceso de restauracion de Pocillopora spp., usando corales de oportunidad (CO), fueron evaluados diversos atributos bioticos en dos sitios a lo largo de un ano, de manera bimensual fueron determinadas las concentraciones de aminoacidos tipo micosporina (AAMs) y niveles de clorofila a (Cl a). De manera especifica, en el ultimo muestreo se realizo un analisis de malonaldehido (MDA) y se calcularon las relaciones ARN/ADN y proteina/ADN como indicadores de estres oxidativo y actividad trascripcional en simbionte y cnidario de todos los morfotipos. En sitio Carrizales (SC) la tecnica de restauracion de ramilletes coralinos (RC) promovio la integracion de COs registrando signos de recuperacion y altas tasas de supervivencia (90%); no obstante, en el Sitio Juluapan (SJ), se observo una falta de respuesta a los indicadores bioticos y moleculares asociados a la recuperacion. Por lo tanto, los CO provenientes de Carrizales no fueron capaces de aclimatarse a un sitio en el que imperan distintas condiciones ambientales. Altas concentraciones de Cl a, AAMs, y una alta relacion ARN/ADN fueron caracteristicas de una condicion saludable de corales, que a su vez, estuvieron asociados a los indicadores de restauracion y mejor supervivencia observada. EnglishDuring a restoration process of Pocillopora spp., using opportunity corals (CO) several biotic attributes were assessed at two sites bi-monthly over a year and concentrations of mycosporin type amino acids (MAAs) and levels of chlorophyll a (Chl a) were determined. Specifically, in the last sampling analysis it was performed an analysis of malonaldehyde (MDA) and the RNA/DNA and protein/DNA relationships were calculated, as an index of oxidative stress and transcriptional activity in symbiont and coral tissue of all morphotypes. At Carrizales site (SC) the coral clusters (RC) used as a method of restoration, promoted the integration of CO showing signs of recovery and high survival rates (90%); however, in Juluapan site (SJ), a lack of response to biotic and molecular indicators associated with recovery was observed. Therefore, CO from Carrizales were not able to acclimate to a place where different conditions prevail. High concentrations of Chl a, AAMs and a high RNA/DNA ratio were characteristic of a healthy condition of coral which in turn, were associated with higher specimen's survival and restoration.