色氨酸覆盖金芳基纳米颗粒与SARS-CoV-2刺突蛋白的能量转移研究

IF 1.6 4区材料科学 Q4 MATERIALS SCIENCE, MULTIDISCIPLINARY

Soft Materials Pub Date : 2022-03-14 DOI:10.1080/1539445X.2022.2053543

Javad B. M. Parambath, S. Kanan, Ahmed Abouzeed Mohamed

{"title":"色氨酸覆盖金芳基纳米颗粒与SARS-CoV-2刺突蛋白的能量转移研究","authors":"Javad B. M. Parambath, S. Kanan, Ahmed Abouzeed Mohamed","doi":"10.1080/1539445X.2022.2053543","DOIUrl":null,"url":null,"abstract":"ABSTRACT Current research on coronavirus 2 disease (SARS-CoV-2) has been focused on developing reliable gold nanomaterials and methods for the detection of viral spike protein (S-proteins). Here, we describe the fluorescence quenching of spike proteins in the presence of robust gold-aryl nanoparticles. The obtained results demonstrated a strong binding affinity between S-proteins and tryptophan immobilized on gold-carbon nanoparticles (Trp-AuNPs) based on the binding constant value of 11.098 M−1. Förster non-radiative energy transfer (FRET) calculations showed an average binding distance of r = 3.06 nm and a critical energy transfer distance of R0 = 2.5 nm. Time-resolved fluorescence studies of conjugated S-proteins onto Trp-AuNPs revealed a short fluorescence lifetime and dynamic quenching.","PeriodicalId":22140,"journal":{"name":"Soft Materials","volume":"20 1","pages":"405 - 413"},"PeriodicalIF":1.6000,"publicationDate":"2022-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Tryptophan capped gold-aryl nanoparticles for energy transfer study with SARS-CoV-2 spike proteins\",\"authors\":\"Javad B. M. Parambath, S. Kanan, Ahmed Abouzeed Mohamed\",\"doi\":\"10.1080/1539445X.2022.2053543\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"ABSTRACT Current research on coronavirus 2 disease (SARS-CoV-2) has been focused on developing reliable gold nanomaterials and methods for the detection of viral spike protein (S-proteins). Here, we describe the fluorescence quenching of spike proteins in the presence of robust gold-aryl nanoparticles. The obtained results demonstrated a strong binding affinity between S-proteins and tryptophan immobilized on gold-carbon nanoparticles (Trp-AuNPs) based on the binding constant value of 11.098 M−1. Förster non-radiative energy transfer (FRET) calculations showed an average binding distance of r = 3.06 nm and a critical energy transfer distance of R0 = 2.5 nm. Time-resolved fluorescence studies of conjugated S-proteins onto Trp-AuNPs revealed a short fluorescence lifetime and dynamic quenching.\",\"PeriodicalId\":22140,\"journal\":{\"name\":\"Soft Materials\",\"volume\":\"20 1\",\"pages\":\"405 - 413\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2022-03-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Soft Materials\",\"FirstCategoryId\":\"88\",\"ListUrlMain\":\"https://doi.org/10.1080/1539445X.2022.2053543\",\"RegionNum\":4,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MATERIALS SCIENCE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Soft Materials","FirstCategoryId":"88","ListUrlMain":"https://doi.org/10.1080/1539445X.2022.2053543","RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MATERIALS SCIENCE, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 2

摘要

摘要当前对冠状病毒2型疾病（严重急性呼吸系统综合征冠状病毒2型）的研究主要集中在开发可靠的金纳米材料和检测病毒刺突蛋白（S蛋白）的方法上。在这里，我们描述了在坚固的金芳基纳米颗粒存在下刺突蛋白的荧光猝灭。所获得的结果表明，基于11.098 M−1的结合常数值，S蛋白与固定在金碳纳米颗粒（Trp-AuNPs）上的色氨酸之间具有很强的结合亲和力。Förster非辐射能量转移（FRET）计算显示平均结合距离r＝3.06nm，临界能量转移距离R0＝2.5nm。结合到Trp-AuNPs上的S-蛋白的时间分辨荧光研究揭示了短的荧光寿命和动态猝灭。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Tryptophan capped gold-aryl nanoparticles for energy transfer study with SARS-CoV-2 spike proteins

ABSTRACT Current research on coronavirus 2 disease (SARS-CoV-2) has been focused on developing reliable gold nanomaterials and methods for the detection of viral spike protein (S-proteins). Here, we describe the fluorescence quenching of spike proteins in the presence of robust gold-aryl nanoparticles. The obtained results demonstrated a strong binding affinity between S-proteins and tryptophan immobilized on gold-carbon nanoparticles (Trp-AuNPs) based on the binding constant value of 11.098 M−1. Förster non-radiative energy transfer (FRET) calculations showed an average binding distance of r = 3.06 nm and a critical energy transfer distance of R0 = 2.5 nm. Time-resolved fluorescence studies of conjugated S-proteins onto Trp-AuNPs revealed a short fluorescence lifetime and dynamic quenching.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Soft Materials 工程技术-材料科学：综合

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

2.2 months

期刊介绍： Providing a common forum for all soft matter scientists, Soft Materials covers theory, simulation, and experimental research in this rapidly expanding and interdisciplinary field. As soft materials are often at the heart of modern technologies, soft matter science has implications and applications in many areas ranging from biology to engineering. Unlike many journals which focus primarily on individual classes of materials or particular applications, Soft Materials draw on all physical, chemical, materials science, and biological aspects of soft matter. Featured topics include polymers, biomacromolecules, colloids, membranes, Langmuir-Blodgett films, liquid crystals, granular matter, soft interfaces, complex fluids, surfactants, gels, nanomaterials, self-organization, supramolecular science, molecular recognition, soft glasses, amphiphiles, foams, and active matter. Truly international in scope, Soft Materials contains original research, invited reviews, in-depth technical tutorials, and book reviews.