MiR-382-5p靶向PDPK1在骨肉瘤(OS)中的抑制功能

Pub Date : 2021-11-25 DOI:10.31901/24566330.2021/21.04.790

Ji-Luan Liu

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引用次数: 0

摘要

miR-382-5p参与骨肉瘤(OS)的发展。然而，miR-382-5p在骨肉瘤中的调控系统尚不清楚。本研究研究了PDPK1与miR- 382-5p在OS中的相互作用。RT-PCR检测miR-382-5p和PDPK1在骨肉瘤细胞和正常人成骨细胞中的表达。双荧光素酶报告基因试验证实PDPK1是miR-382-5p的靶标。CCK-8评价细胞活力。流式细胞术测定细胞凋亡率。Transwell和Scratch试验估计细胞转移。miR-382-5p在OS细胞中被抑制。进一步的功能结果显示，miR-382-5p上调通过介导PDPK1在OS细胞中降低细胞活力和流动性

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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MiR-382-5p’s Function as a Suppressor in Osteosarcoma (OS) by Targeting PDPK1

ABSTRACT miR-382-5p engages in development of osteosarcoma (OS). However, the regulatory system of miR-382-5p in osteosarcoma remains to be revealed. This research studied the interplay between PDPK1 and miR- 382-5p in OS. RT-PCR was used to evaluate miR-382-5p and PDPK1 expression in OS cells and normal human osteoblast cells. Dual-luciferase reporter assay validated PDPK1 as a miR-382-5p target. CCK-8 evaluated the cell viability. Flow cytometric method determined cell apoptosis rate. Transwell and Scratch assays estimated the cell metastasis. miR-382-5p was inhibited in OS cells. Further functional results showed miR-382-5p upregulation reduced cell viability, and mobility by mediating PDPK1 in OS cells

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