miR-625-3p调节转录因子21 (TCF21)表达增强肝癌细胞放射抵抗

Pub Date : 2022-02-10 DOI:10.31901/24566330.2022/22.01.785

Jiongbo Lou

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引用次数: 0

摘要

摘要miR-625-3p在各种癌症中调节细胞功能。然而，它在肝癌放射抵抗中的作用仍有待揭示。本研究旨在探讨miR-625-3p在肝癌中的作用。RTPCR检测miR-625-3p/TCF21在肝癌组织和细胞系中的表达。MTT法分析了miR-625-3p在肝癌细胞无线电抗性调节中的作用。评估患者的总生存率与miR-625-3p的相关性。萤光素酶报告基因实验验证了预测的TCF21为miR-625-3p靶点。在肝癌患者中发现了更高的miR-625-3p表达。在耐辐射患者的组织中，miR-625-3p较高。miR-625-3p的过表达增加了肝癌细胞对辐射的放射抗性。miR-625-3p通过与TCF21的3’UTR结合而不利地调节TCF21。下调miR-625-3p促进了细胞对辐射的敏感性，而TCF21的抑制则逆转了这种敏感性。

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miR-625-3p Regulates Transcription Factor 21 (TCF21) Expression to Enhance Radio-resistance of Hepatoma Cells

ABSTRACT miR-625-3p regulates cell functions in various cancers. However, its role in radio-resistance in hepatoma remains to be unveiled. This study aims at investigating the functions of miR-625-3p in hepatoma. RTPCR detected miR-625-3p/TCF21 expression in hepatoma tissues and cell line. MTT assays were conducted to analyze the miR-625-3p role in regulations of radio-resistance of hepatoma cells. Overall survival was evaluated in correlation to miR-625-3p among patients. Luciferase reporter experiments validated the predicted TCF21 as miR-625-3p target. Higher miR-625-3p expression was discovered in hepatoma patients. In tissues of radio resistant patients, miR-625-3p was higher. The overexpression of miR-625-3p increased the radio-resistance of hepatoma cells to irradiation. miR-625-3p adversely moderated TCF21 via binding to the 3’UTR of TCF21. Downregulating miR-625-3p promoted the cell sensitivity to irradiation, which was reversed by TCF21 inhibition.

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