阿曲班在大鼠脊髓损伤修复中的作用

中华创伤杂志 Pub Date : 2020-01-15 DOI:10.3760/CMA.J.ISSN.1001-8050.2020.01.018

Chenxi Zhao, Baoyou Fan, Wenxiang Li, Xu Wang, Yilin Pang, Xue Yao, S. Feng

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HE staining was used to compare the size of the cavity in the local region 42 days after injury. \n \n \nResults \nAt day 7 after injury, the BBB score was (3.7±0.5)points and the inclined plane test was (28.0±2.6)° in the Argatroban group, which were better than those in the injury group [(3.3±0.5)points, (24.3±1.9)°] (P<0.05). At day 42 after injury, the BBB score was (13.0±0.8)points and inclined plane test was (50.7±2.7)° in the Argatroban group, which were significantly better than those in the injury group [(9.7±1.3) points, (40.5±2.7)°] (P<0.05). But all the above values in the Argatroban group were significantly lower than those in the sham group [(21.0±0.0)points, (60.0±0.0)°](P<0.05). At day 42 after operation, the SEP latency [(25.0±0.9)ms] in the Argatroban group was significantly shorter than that in the injury group [(31.5±1.9) ms]; the amplitude [(2.1±0.1)μV] in the Argatroban group was lower than that in the injury group [(0.5±0.1)μV] (P<0.05). 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引用次数: 1

摘要

目的探讨阿加曲班对大鼠脊髓损伤的修复作用。方法选择雌性Wistar大鼠54只，按随机数字表法分为假手术组、损伤组和阿加曲班组，每组18只。假手术组仅取t10椎板;损伤组采用脊髓损伤装置制作大鼠脊髓损伤模型;阿加曲班组脊髓损伤后给予阿加曲班治疗。分别于伤前和伤后7、14、21、28、35、42 d采用BBB评分和斜板试验评价后肢运动功能恢复情况。术后42 d检测感觉诱发电位(SEP)和运动诱发电位(MEP)。HE染色比较损伤后42天局部腔的大小。结果损伤后第7天，阿加曲班组BBB评分为(3.7±0.5)分，斜面测试为(28.0±2.6)°，优于损伤组[(3.3±0.5)分，(24.3±1.9)°](P<0.05)。损伤后第42天，阿加曲班组BBB评分为(13.0±0.8)分，斜面测试为(50.7±2.7)°，显著优于损伤组[(9.7±1.3)分，(40.5±2.7)°](P<0.05)。但阿加曲班组上述数值均显著低于假手术组[(21.0±0.0)点，(60.0±0.0)°]，差异有统计学意义(P<0.05)。术后第42天，阿加曲班组SEP潜伏期[(25.0±0.9)ms]明显短于损伤组[(31.5±1.9)ms];阿加曲班组的振幅[(2.1±0.1)μV]低于损伤组[(0.5±0.1)μV] (P<0.05)。阿加曲班组MEP潜伏期[(11.5±1.0)ms]明显短于损伤组[(17.5±1.1)ms]，振幅[(4.8±0.8)μV]明显低于损伤组[(2.8±0.7)μV] (P<0.05)。与假手术组相比，阿加曲班组SEP或MEP潜伏期和振幅有显著性差异[(7.5±1.0)ms，(7.5±1.0)μV](P<0.05)。HE染色显示，阿加曲班组病变中心面积[(0.35±0.04)mm2]明显小于损伤组[(0.71±0.05)mm2]。结论脊髓损伤后，阿加曲班能有效保护损伤区脊髓组织，促进后肢感觉和运动功能的恢复。关键词:脊髓损伤;Argatroban;炎症;凝血酶

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Role of argatroban in repair of spinal cord injury in rats

Objective To investigate the effect of argatroban in repair of spinal cord injury in rats. Methods A total of 54 female Wistar rats were selected and divided into three groups according to the random number table: sham group, injury group and Argatroban group, with 18 rats in each group. The sham group only took the T10lamina; the injury group used the spinal cord injury device to make the rat spinal cord injury model; the Argatroban group received Argatroban treatment after spinal cord injury. The recovery of hindlimb motor function was evaluated by BBB score and clined plate test before injury and 7, 14, 21, 28, 35 and 42 days after injury. The sensory evoked potentials (SEP) and motor evoked potentials (MEP) were detected 42 days after operation. HE staining was used to compare the size of the cavity in the local region 42 days after injury. Results At day 7 after injury, the BBB score was (3.7±0.5)points and the inclined plane test was (28.0±2.6)° in the Argatroban group, which were better than those in the injury group [(3.3±0.5)points, (24.3±1.9)°] (P<0.05). At day 42 after injury, the BBB score was (13.0±0.8)points and inclined plane test was (50.7±2.7)° in the Argatroban group, which were significantly better than those in the injury group [(9.7±1.3) points, (40.5±2.7)°] (P<0.05). But all the above values in the Argatroban group were significantly lower than those in the sham group [(21.0±0.0)points, (60.0±0.0)°](P<0.05). At day 42 after operation, the SEP latency [(25.0±0.9)ms] in the Argatroban group was significantly shorter than that in the injury group [(31.5±1.9) ms]; the amplitude [(2.1±0.1)μV] in the Argatroban group was lower than that in the injury group [(0.5±0.1)μV] (P<0.05). The MEP latency [(11.5±1.0)ms] in the Argatroban group was significantly shorter than that in the injury group [(17.5±1.1)ms], and the amplitude [(4.8±0.8)μV] in the Argatroban group was lower than that in the injury group [(2.8±0.7)μV] (P<0.05). And the SEP or MEP latency and amplitude in the Argatroban group showed significant differences compared to the sham group [(7.5±1.0)ms, (7.5±1.0)μV](P<0.05). HE staining showed that the central area of the lesion in the Argatroban group [(0.35±0.04)mm2] was significantly smaller than that in the injury group [(0.71±0.05)mm2]. Conclusion After spinal cord injury, argatroban can protect the spinal cord tissue effectively in the injured area and promote recovery of sensory and motor function in the hind limbs of rats. Key words: Spinal cord injuries; Argatroban; Inflammation; Thrombin

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中华创伤杂志

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期刊介绍： Chinese Journal of Trauma (International Standard Serial Publication Number: ISSN 1001-8050, Domestic Uniform Serial Publication Number: CN 50-1098/R) was founded in September 1985, which is the only high-level medical professional academic journal that can comprehensively and systematically reflect the achievements and development trends of China's traumatology medicine, and has a wide academic influence in China's traumatology medicine community. It has a wide range of academic influence in China's trauma medicine. Chinese Journal of Trauma is a source journal of China Science and Technology Paper Statistics, a source journal of China Science Citation Database (CSCD), a core journal of China Comprehensive Medicine and Health Care, a source journal of China Academic Journals Comprehensive Evaluation Database (CAJCED), a full-text journal of China Journal Full-text Database (CJFD), a core academic journal of China Center for Scientific Evaluation (RCCSE), a core academic journal of China Traumatology and Traumatology Center (CTC), a core academic journal of China Traumatology Center (RCCSE). RCCSE) core academic journals; Chinese Biomedical Journal Database (CMCC), Chinese Biomedical Journal Citation Database (CBJCED), China Journal Network (CJN), China Academic Journals (CD-ROM), Chinese Academic Journals Abstracts (Chinese Edition), Chemical Abstracts of the United States (CA), Index Copernicus of Poland (IC), and Japan Institute of Science and Technology Database (JICST), World Health Organization Western Pacific Region Medical Search (WPRIM) and Russian Journal of Abstracts (ΡЖ) included journals.