Zostera japonica DnaJ基因增强转基因拟南芥的耐盐性

IF 0.9 4区 生物学 Q4 PLANT SCIENCES
Siting Chen, Guanglong Qiu
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引用次数: 0

摘要

土壤盐害已成为影响植物种子萌发、作物生长和产量的主要生物胁迫之一。盐损伤可导致植物中超氧阴离子、过氧化氢和羟基自由基的积累,大量积累的活性氧会引起脂质、蛋白质和核酸的氧化,进而引起细胞损伤。在本实验中,以转基因拟南芥(Z.japonica)DnaJ基因ZjDjB1过表达的幼苗和成株为材料。用100 mM和200 测定了mM NaCl和根长、鲜重、叶片含水量和干重、生理指标和抗氧化系统酶活性。结果表明,过表达J.japonica DnaJ基因ZjDjB1可以显著减轻盐胁迫对转基因ZjDjB1拟南芥幼苗和成株的损伤。结果表明,在盐胁迫下,过表达日本血吸虫DnaJ基因ZjDjB1可以降低MDA和O2-的含量,增加脯氨酸的含量,激活抗氧化系统的酶活性,减轻盐胁迫对植物的损害。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Enhancement of salt tolerance of transgenic Arabidopsis thaliana by Zostera japonica DnaJ gene
Soil salt damage has become one of the main biological stresses affecting plant seed germination, crop growth and yield. Salt damage can lead to the accumulation of superoxide anion, hydrogen peroxide and hydroxyl free radicals in plants, and a large amount of accumulated reactive oxygen species will cause the oxidation of lipids, proteins and nucleic acids and then cause cell damage. In this experiment, the seedlings and adult plants of transgenic Arabidopsis with overexpression of Zostera japonica (Z. japonica) DnaJ gene ZjDjB1 were used as materials. Wild-type and transgenic ZjDjB1 Arabidopsis were treated with 100 mM and 200 mM NaCl and root length, fresh weight, leaf water content and dry weight, physiological indexes and antioxidant system enzyme activities were measured. The results showed that overexpression of Z. japonica DnaJ gene ZjDjB1 could significantly alleviate the damage caused by salt stress to transgenic ZjDjB1 Arabidopsis seedlings and adult plants. The results showed that overexpression of Z. japonica DnaJ gene ZjDjB1 could reduce the content of MDA and O2-, increase the content of proline and activate the enzyme activity of antioxidant system under salt stress, to reduce the damage of salt stress to plants.
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来源期刊
Israel Journal of Plant Sciences
Israel Journal of Plant Sciences 生物-植物科学
CiteScore
1.90
自引率
0.00%
发文量
17
审稿时长
>12 weeks
期刊介绍: The Israel Journal of Plant Sciences is an international journal of extensive scope that publishes special issues dealing with all aspects of plant sciences, including but not limited to: physiology, cell biology, development, botany, genetic
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