变形链球菌gtfB家族分离基因的分子特征分析

Q4 Dentistry

Revista Odonto Ciencia Pub Date : 2018-12-30 DOI:10.15448/1980-6523.2018.1.29727

Mônica Moreira, M. M. Nascimento, M. M. Poletto, Débora do Rocio Kliisiowicz, R. R. Gomes, V. Vicente

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引用次数: 0

摘要

目的：龋病是一种多因素感染性疾病，其主要病原体是变形链球菌，其毒力因子使其能够粘附在牙釉质上，并通过产生细胞外多糖促进牙齿生物膜的形成。变异链球菌的传播可能发生在人与人之间，通常发生在家庭内部。本研究旨在通过对编码葡糖基转移酶β（gtfB）的基因进行部分测序来评估变异链球菌的家族内遗传模式。方法：我们先前分析了来自9个家族的40个个体的唾液样本，获得了64个生物化学特征为变异链球菌的分离株。采用随机扩增多态性DNA（RAPD）对分离株进行鉴定。通过16S rRNA基因部分序列和gtfB基因测序来表征那些具有更大相似性的基因。结果：从具有龋齿活性的个体中分离的菌株之间存在遗传相似性；而来自无龋个体的分离株显示它们在基因上是不同的，这表明它们具有不同的毒力模式。结论：目前的结果表明，gtfB基因的部分测序是研究变异链球菌遗传模式和毒力的潜在标志物，值得进一步研究，以确定有龋齿风险的家族。

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Molecular characterization of Streptococcus mutans gtfB gene isolated from families

OBJECTIVE: Caries is a multifactorial infectious disease and the main etiological agent is the bacteria Streptococcus mutans due to its virulence factors, which enable the adherence to dental enamel and favours the formation of dental biofilm through the production of extracellular polysaccharides. The transmission of S. mutans can occur between people, often within families. The present study aimed to evaluate the intrafamily genetic pattern of S. mutans through partial sequencing of the gene that encodes the glucosyltransferase β (gtfB) enzyme.METHODS: We previously analyzed saliva samples from 40 individuals from nine families, and it was obtained 64 isolates biochemically characterized as S. mutans. The isolates were evaluated by random amplification of polymorphic DNA (RAPD). Those with greater similarity were characterized by partial sequence 16S rRNA gene and the gtfB gene sequencing.RESULTS: It was observed genetic similarity among strains isolated from individuals with caries activity; while isolates from individual without caries showed that they are genetically distinct, suggesting a different virulence pattern.CONCLUSION: The present results demonstrated that partial sequencing of the gtfB gene showed to be a potential marker to investigate genetic pattern and virulence of S. mutans, deserving further investigation in order to identify families at risk of caries.

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来源期刊

Revista Odonto Ciencia Dentistry-Dentistry (all)

CiteScore

0.30

自引率

0.00%

发文量

期刊介绍： Cessation. The Revista Odonto Ciência is a peer-reviewed journal published online (ISSN 1980-6523), four times per year, supported by the Pontifical Catholic University of Rio Grande do Sul – Dental School since 1986. Its abbreviation is Rev Odonto Cienc, which should be used in references, bibliographic legends, and footnotes. The Revista Odonto Ciência is an international scientific journal dedicated to provide a forum for academics, researchers, and practitioners to communicate relevant basic, clinical, education, and epidemiological studies in Dentistry and related fields. The Journal seeks to publish articles on new conceptual models, innovative technology, alternative procedures or treatment to reduce disease burden in the general population or specific groups, thus contributing to translating science into practice and social development. Contributions falling into the following categories will be considered for publication: 1) original research reports of basic and applied oral sciences, epidemiological, and education studies; 2) review article; 3) case report. The Revista Odonto Ciência only accepts manuscripts written in English. Manuscripts submitted to this journal should not have been published before nor have publication pending elsewhere.