杂化单色二氧化硅纳米颗粒标记大肠杆菌的自组装动力学及其对合成纳米生物光学性能的影响

IF 1.1 4区 物理与天体物理 Q4 NANOSCIENCE & NANOTECHNOLOGY
Luna R. Gomez Palacios, Carina Salinas, A. Veglia, M. Amé, A. Bracamonte
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引用次数: 1

摘要

摘要研究了二氧化硅纳米颗粒在大肠杆菌上的沉积。硅化表面与生物膜极性组分之间的非共价相互作用形成了纳米生物结构。用不同的显微镜技术,如亮场共聚焦显微镜和透射电子显微镜(TEM),评估了这种混合结构的稳定构象特征。纳米生物结构在胶体分散体和没有水介质的情况下被检测到。纳米生物相互作用与强极性和氢桥的非共价相互作用有关。因此,在最佳条件下制备后,通过亮场共聚焦显微镜和透射电镜观察到结构良好的纳米生物结构。然而,为了评估它们的稳定性和纳米生物结构之间的相互作用,确定了不同时期的尺寸分布。根据纳米颗粒和细菌浓度记录不同的纳米生物聚集体大小。单纳米标记的大肠杆菌具有良好的分散性,细菌浓度低。在中、高浓度条件下,纳米生物结构在不同时期的分布也不同。结果表明,二氧化硅纳米颗粒在大肠杆菌中的掺入提高了其分散性;然而,随着时间的推移,他们用硅纳米表面修饰的大肠杆菌膜增强了它们之间的纳米生物结构相互作用。在这里,我们讨论了大肠杆菌的动力学和纳米生物光学特性。它们的纳米生物结构不能被认为是静态系统;它们的相互作用被认为是分散性、稳定性和对抗其他化学制剂(如抗生素)的重要因素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Self-assembly dynamics and effect on synthetic nanobio-optical properties by hybrid monocolored silica nanoparticle labeling of Escherichia coli
Abstract. The deposition of silica nanoparticles on Escherichia coli bacteria was investigated. The noncovalent interaction between the silanized surfaces and polar components of the biomembrane resulted in a nanobiostructure. This hybrid architecture showed stable conformation characteristics evaluated with different microscopy techniques, such as bright field confocal microscopy and transmission electron microscopy (TEM). Nanobioarchitectures were detected within colloidal dispersions and in the absence of aqueous media. Nanobiointeractions were related to strong polar and hydrogen bridges’ noncovalent interactions. Thus, well-constituted and defined nanobiostructures were observed by bright field confocal microscopy and TEM after their preparation in optimal conditions. However, to evaluate their stability and internanobiostructure interactions, size distributions within variable periods were determined. Variable nanobioaggregate sizes were recorded according to nanoparticles and bacteria concentrations. From single nanolabeled E. coli with well dispersible properties, low bacteria concentrations were observed. In intermediate and high concentrations, different distributions of nanobiostructures were observed in different periods. It was observed that the incorporation of silica nanoparticles into E. coli increased their dispersibility; however, their modified E. Coli membranes with silanized nanosurfaces augmented their internanobiostructure interactions through time. Here, we discuss the dynamics and nanobio-optics properties of E. coli. Their nanobiostructures could not be considered to be static systems; their interactions are regarded as important factors for dispersibility, stability, and effects against additional chemical agents such as antibiotics.
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来源期刊
Journal of Nanophotonics
Journal of Nanophotonics 工程技术-光学
CiteScore
2.60
自引率
6.70%
发文量
42
审稿时长
3 months
期刊介绍: The Journal of Nanophotonics publishes peer-reviewed papers focusing on the fabrication and application of nanostructures that facilitate the generation, propagation, manipulation, and detection of light from the infrared to the ultraviolet regimes.
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