同时,采用光镊和荧光杂交的单分子方法

IF 2.7
Guoteng Ma , Chunguang Hu , Shuai Li , Xiaoqin Gao , Hongbin Li , Xiaotang Hu
{"title":"同时,采用光镊和荧光杂交的单分子方法","authors":"Guoteng Ma ,&nbsp;Chunguang Hu ,&nbsp;Shuai Li ,&nbsp;Xiaoqin Gao ,&nbsp;Hongbin Li ,&nbsp;Xiaotang Hu","doi":"10.1016/j.npe.2019.11.004","DOIUrl":null,"url":null,"abstract":"<div><p>As studies on life sciences progress toward the single-molecule level, new experiments have put forward more requirements for simultaneously displaying the mechanical properties and conformational changes of biomolecules. Optical tweezers and fluorescence microscopy have been combined to solve this problem. The combination of instruments forms a new generation of hybrid single-molecule technology that breaks through the limitations of traditional biochemical analysis. Powerful manipulation and fluorescence visualization have been widely used, and these techniques provide new possibilities for studying complex biochemical reactions at the single-molecule level. This paper explains the features of this combined technique, including the application characteristics of single-trap and dual-traps, the anti-bleaching method, and optical tweezers combined with epi-fluorescence, confocal fluorescence, total internal reflection fluorescence, and other fluorescence methods. Using typical experiments, we analyze technical solutions and explain the factors and principles that instrument designers should consider. This review aims to give an introduction to this novel fusion technology process and describe important biological results.</p></div>","PeriodicalId":87330,"journal":{"name":"Nanotechnology and Precision Engineering","volume":"2 4","pages":"Pages 145-156"},"PeriodicalIF":2.7000,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.npe.2019.11.004","citationCount":"6","resultStr":"{\"title\":\"Simultaneous, hybrid single-molecule method by optical tweezers and fluorescence\",\"authors\":\"Guoteng Ma ,&nbsp;Chunguang Hu ,&nbsp;Shuai Li ,&nbsp;Xiaoqin Gao ,&nbsp;Hongbin Li ,&nbsp;Xiaotang Hu\",\"doi\":\"10.1016/j.npe.2019.11.004\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>As studies on life sciences progress toward the single-molecule level, new experiments have put forward more requirements for simultaneously displaying the mechanical properties and conformational changes of biomolecules. Optical tweezers and fluorescence microscopy have been combined to solve this problem. The combination of instruments forms a new generation of hybrid single-molecule technology that breaks through the limitations of traditional biochemical analysis. Powerful manipulation and fluorescence visualization have been widely used, and these techniques provide new possibilities for studying complex biochemical reactions at the single-molecule level. This paper explains the features of this combined technique, including the application characteristics of single-trap and dual-traps, the anti-bleaching method, and optical tweezers combined with epi-fluorescence, confocal fluorescence, total internal reflection fluorescence, and other fluorescence methods. Using typical experiments, we analyze technical solutions and explain the factors and principles that instrument designers should consider. This review aims to give an introduction to this novel fusion technology process and describe important biological results.</p></div>\",\"PeriodicalId\":87330,\"journal\":{\"name\":\"Nanotechnology and Precision Engineering\",\"volume\":\"2 4\",\"pages\":\"Pages 145-156\"},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2019-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.npe.2019.11.004\",\"citationCount\":\"6\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nanotechnology and Precision Engineering\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2589554019300406\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nanotechnology and Precision Engineering","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2589554019300406","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 6

摘要

随着生命科学研究向单分子水平发展,新的实验对同时显示生物分子的力学性质和构象变化提出了更多的要求。光学镊子和荧光显微镜相结合来解决这个问题。这些仪器的组合形成了新一代杂交单分子技术,突破了传统生化分析的局限性。强大的操作和荧光可视化技术得到了广泛的应用,这些技术为在单分子水平上研究复杂的生化反应提供了新的可能性。本文阐述了该组合技术的特点,包括单阱和双阱的应用特点、抗漂白方法、光镊与外延荧光、共聚焦荧光、全内反射荧光等荧光方法的结合。通过典型实验,分析了技术解决方案,说明了仪器设计人员应考虑的因素和原则。本文旨在介绍这种新型融合技术的过程和描述重要的生物学结果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Simultaneous, hybrid single-molecule method by optical tweezers and fluorescence

As studies on life sciences progress toward the single-molecule level, new experiments have put forward more requirements for simultaneously displaying the mechanical properties and conformational changes of biomolecules. Optical tweezers and fluorescence microscopy have been combined to solve this problem. The combination of instruments forms a new generation of hybrid single-molecule technology that breaks through the limitations of traditional biochemical analysis. Powerful manipulation and fluorescence visualization have been widely used, and these techniques provide new possibilities for studying complex biochemical reactions at the single-molecule level. This paper explains the features of this combined technique, including the application characteristics of single-trap and dual-traps, the anti-bleaching method, and optical tweezers combined with epi-fluorescence, confocal fluorescence, total internal reflection fluorescence, and other fluorescence methods. Using typical experiments, we analyze technical solutions and explain the factors and principles that instrument designers should consider. This review aims to give an introduction to this novel fusion technology process and describe important biological results.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信