环状rna和环境及临床因素在椎间盘退变中的潜在作用

IF 1.3 Q4 ENVIRONMENTAL SCIENCES
Mahshid Malakootian, Akram Gholipour, Mahrokh Bagheri Moghaddam, M. Arabian, Maziar Oveisee
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引用次数: 3

摘要

背景:椎间盘退变(IDD)是劳动年龄人群中常见的残疾。IDD的潜在发病机制需要阐明。本研究旨在通过生物信息学方法确定IDD中差异表达的环状RNA(circRNA)。此外,还对参与IDD发病机制的环境和临床因素进行了综述。方法:从基因表达综合征(GEO)中获得IDD患者和健康人的circRNA阵列图谱(GSE67566)。GEO2R用于分析circRNA的表达谱。对差异表达最高的circRNA进行了功能计算机分析。通过PubMed和Google Scholar对环境和临床因素进行了综述。结果:25个circRNA在IDD中有差异表达。两种circRNA(hsa_ circRNA_101645和hsa_cirRNA_101852)表现出最多的下调和上调表达。功能计算机分析表明,上述circRNA含有AGO2和EIF4A3的靶位点以及几种微小RNA。结果表明,这2种环状circRNA可能吸收hsa-miR-330-3p、hsa-miR-502-5p、hsa-miR-662、hsa-miR-874和hsa-miR-646,并调节PSD3、SIK2、PCYT1B、ARID5B、MTMR3和HIPK2的表达,这些表达在自噬和细胞衰老中起着重要作用。温度、重金属暴露、年龄、超重、职业、运动、高血压和吸烟是与IDD进展相关的环境和临床因素。结论:尽管这些结果需要通过实验分析来证实,但它们反映了特定circRNA在IDD发病机制中的可能作用。关于IDD与环境和临床因素之间关系的争议需要深入的人群研究。研究circRNAs等新的分子调控标记物可以阐明IDD的潜在分子机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Potential Roles of Circular RNAs and Environmental and Clinical Factors in Intervertebral Disc Degeneration
Background: Intervertebral disc degeneration (IDD) is a common disability in the working-age population. The underlying pathogenesis of IDD needs elucidation. This study aimed to determine differentially expressed circular RNAs (circRNAs) in IDD by bioinformatics. Additionally, the environmental and clinical factors involved in IDD pathogenesis were reviewed. Methods: The circRNA array profiling of patients with IDD and healthy individuals (GSE67566) was acquired from Gene Expression Omnibus (GEO). GEO2R was employed to analyze the expression profiles of the circRNAs. Functional in silico analysis was done on circRNAs with the highest differential expression. Environmental and clinical factors were reviewed through PubMed and Google Scholar. Results: Twenty-five circRNAs were differentially expressed in IDD. Two circRNAs (hsa_ circRNA_101645 and hsa_circRNA_101852) exhibited the most downregulated and upregulated expressions. The functional in silico analysis showed that the aforementioned circRNAs harbored target sites for AGO2 and EIF4A3 and several microRNAs. The upshots indicated that these 2 circular circRNAs might sponge hsa-miR-330-3p, hsa-miR-502-5p, hsa-miR-662, hsa-miR-874, and hsa-miR-646 and regulate PSD3, SIK2, PCYT1B, ARID5B, MTMR3, and HIPK2 expressions, which play significant roles in autophagy and cellular senescence. Temperature, heavy metal exposure, age, overweight, occupation, exercise, hypertension, and smoking were the environmental and clinical factors associated with IDD progression. Conclusion: Although the results need confirmation by experimental analysis, they reflect the possible role of particular circRNAs in IDD pathogenesis. The controversy concerning the association between IDD and environmental and clinical factors necessitates in-depth population research. Investigating novel molecular regulatory markers like circRNAs could clarify the underlying molecular mechanisms of IDD.
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来源期刊
CiteScore
2.40
自引率
37.50%
发文量
17
审稿时长
12 weeks
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