Alhagie Cham, M. Zacarías, H. L. Saldaña, R. E. Vázquez Alvarado, Emilio Olivares Sáenz, G. Martínez-Ávila, Omar Guadalupe Alvarado Gómez
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In this study we investigated the effects of elicitors (Activane®, Micobiol®, Stemicol® in doses of 1.8 g L–1, 3 m L–1 and 2.5 g·L–1) on the activity of two enzymes (polyphenoloxydase and peroxidase) in leaves as well as on the bioactive content (DPPH scavenging activity, total phenol and total flavonoid content) in fruits of Solanum lycopersicum L. The elicitors studied affected the production of enzyme and antioxidant activities in tomato, though showed a variable influence on the production of total phenolic and flavonoid content in tomato fruits. In general, the elicitors are able to increase polyphenoloxydase activity in leaves and phytochemical properties in fruits, with a significant negative correlation between the DPPH scavenging activity, total phenolic and flavonoids. 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引用次数: 1
摘要
植物在受到病原体攻击后,通过被动防御和主动防御机制进行自我保护。用各种药剂处理植物,包括细胞壁碎片、植物提取物和合成化学品,可以诱导局部和全身抵抗随后的病原体攻击。鉴于水果和蔬菜中发现的植物化学化合物对消费者的健康有不同的益处,因此有不同的策略来提高这些化合物的浓度,其中使用激发剂。诱导已被用于触发植物不同的防御反应,导致不同的机制,如与次生代谢相关的酶的激活。本研究研究了1.8 g L-1、3 m L-1和2.5 g·L-1剂量的激发子Activane®、Micobiol®、Stemicol®对番茄叶片中两种酶(多酚氧化酶和过氧化物酶)活性以及果实中生物活性(DPPH清除活性、总酚和总黄酮含量)的影响。虽然对番茄果实中总酚和类黄酮含量的产生有不同的影响。总的来说,激发子能够提高叶片的多酚氧化酶活性和果实的植物化学性质,DPPH清除活性与总酚和总黄酮呈显著负相关。这些发现为这些激发子在番茄(‘Bola’和‘Saladette’)温室生产中的应用提供了积极的指导。亮点-激发子增加番茄叶片中PPO的酶活性。-总酚含量和类黄酮的产生取决于激发剂的类型。-对番茄叶片过氧化物酶含量无影响。-不同处理间果实水提取物的抗氧化能力差异显著(P = 0.05)。-总酚含量与类黄酮含量呈正相关。
Potential elicitors on secondary metabolite production and antioxidant defence activity of two tomato (Solanum lycopersicum L.) varieties
Plants protect themselves, after pathogen attack, through the passive and active defence mechanisms. The treatment of plants with various agents, including cell wall fragments, plant extracts, and synthetic chemicals, can induce resistance to subsequent pathogen attack both locally and systemically. In view of the capability of phytochemicals compounds found in fruits and vegetables with different proven health benefits to consumers, there are different strategies to enhance the concentration of these compounds, among which the use of elicitors. Elicitation has been used to trigger different defence responses in plants, which lead to different mechanisms, such as activation of enzymes related with secondary metabolism. In this study we investigated the effects of elicitors (Activane®, Micobiol®, Stemicol® in doses of 1.8 g L–1, 3 m L–1 and 2.5 g·L–1) on the activity of two enzymes (polyphenoloxydase and peroxidase) in leaves as well as on the bioactive content (DPPH scavenging activity, total phenol and total flavonoid content) in fruits of Solanum lycopersicum L. The elicitors studied affected the production of enzyme and antioxidant activities in tomato, though showed a variable influence on the production of total phenolic and flavonoid content in tomato fruits. In general, the elicitors are able to increase polyphenoloxydase activity in leaves and phytochemical properties in fruits, with a significant negative correlation between the DPPH scavenging activity, total phenolic and flavonoids. These findings provide positive directions for the possible use of these elicitors in tomato (‘Bola’ and ‘Saladette’) production in greenhouse.
Highlights - Elicitors increase the enzymatic activity of PPO in tomato leaves. - The production of total phenolic contents and flavonoids depends on type of elicitors. - No effects on the peroxidase content of the tomato leaves. - The antioxidant capacity of the aqueous fruits extract showed a significant difference (P = 0.05) between the treatments in the Saladette variety. - Positive relationship between total phenolic contents and flavonoids.
期刊介绍:
The Italian Journal of Agronomy (IJA) is the official journal of the Italian Society for Agronomy. It publishes quarterly original articles and reviews reporting experimental and theoretical contributions to agronomy and crop science, with main emphasis on original articles from Italy and countries having similar agricultural conditions. The journal deals with all aspects of Agricultural and Environmental Sciences, the interactions between cropping systems and sustainable development. Multidisciplinary articles that bridge agronomy with ecology, environmental and social sciences are also welcome.