伊比利亚Knap杂草（Centaurea section.Jacea and Lepteranthus，Astraceae）的分类分化和亚特异性花形态类型的遗传分离1

IF 1.1 3区生物学 Q3 PLANT SCIENCES

Annals of the Missouri Botanical Garden Pub Date : 2020-11-25 DOI:10.3417/2020389

Itziar Arnelas, E. Pérez-Collazos, J. A. Devesa, A. Manzaneda, P. Catalán

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引用次数: 2

摘要

摘要Centaurea L.种和种下分类群的分类边界经常因杂交而变得模糊。然而，平衡遗传隔离有助于保持分类限制和促进快速的物种形成过程。辐射状（R）和非辐射状（NR）头状花序是Centaurea的两种主要花型。然而，目前尚不清楚基因流如何影响种群和分类群中花形态类型的分布。我们研究了伊比利亚Centaurea教派R和NR种群的分类分化和遗传隔离的潜在影响。Jacea（Mill.）Pers.ex Dumot。和Lepteranthus（Neck.ex DC.）Dumot。使用165个扩增片段长度多态性（AFLP）标记对来自58个种群（12个分类群）的510个个体进行了分析。遗传多样性和结构参数在分类单元、种群和花形态型水平上进行了估计。我们使用基于距离的冗余分析（dbRDA）测试了群体成对固定指数（Fst）遗传距离与近交系数（Fis）之间是否存在相关性，近交系数被视为各组R和NR形态类型之间生殖隔离的替代品，并考虑了地理距离。我们还在不同花形态类型之间进行了距离隔离（IBD）测试，旨在推断遗传隔离对个体进化和分类差异的影响。Centaurea教派的分类群。Jacea和Lepterasus构成了独立的进化谱系。大多数检测到的基因簇与我们最近的研究中提出的分类界限相匹配。C.debeauxii Godr的遗传多样性最高。&Gren。亚种。debeauxii和subsp。grandiflora（Gaudin ex Schübl.&G.Martens）Devesa&Arnelas和C.jacea L.亚种。angustifolia（DC）Gremli（R）和C.nevadensis Boiss最低Reut。（NR）在Centaurea教派内部。Jacea分支，而在Centaurea教派内的C.linifolia L.中最高。细尾目分支。在任何研究病例中，群体水平的dbRDA测试都没有检测到R与NR遗传距离和Fis值的显著相关性；相比之下，他们检测到了与经度（C.jacea subsp.angustifolia，C.debeauxii，C.linifolia–C.stuessyi Arnelas，Devesa&E.López）或纬度（C.nigra L.）的显著相关性。个体水平的IBD分析表明，除了地理因素外，其他形态类型相关因素可能影响C.nigra，C.debeaxii，和C.linifolia–C。施。这些结果表明，不同形态在同一分类单元或类群内的固定可以用地理隔离来解释，尽管我们不能忽视其他潜在的未知因素，这些因素可能导致了这些最近分化的种群的微分化，这些种群起源于数千年前。

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Taxonomic Differentiation of Iberian Knapweeds (Centaurea sects. Jacea and Lepteranthus, Asteraceae) and Genetic Isolation of Infraspecific Floral Morphotypes1

Abstract The taxonomic boundaries of Centaurea L. species and infraspecific taxa are often blurred by hybridizations. However, counterbalancing genetic isolation contributes to maintaining taxonomic limits and fostering rapid speciation processes. Radiant (R) and non-radiant (NR) capitula are two major floral morphs present in Centaurea. However, it is unclear how gene flow affects the distribution of floral morphotypes across populations and taxa. We have investigated the taxonomic differentiation and potential effect of genetic isolation in R and NR populations of Iberian Centaurea sects. Jacea (Mill.) Pers. ex Dumort. and Lepteranthus (Neck. ex DC.) Dumort. A total of 510 individuals from 58 populations (12 taxa) were analyzed using 165 amplified fragment length polymorphism (AFLP) markers. Genetic diversity and structure parameters were estimated at the taxon, population, and floral morphotypic levels. We tested whether there was correlation between population pairwise fixation index (Fst) genetic distances and the inbreeding coefficient (Fis), taken as a surrogate of reproductive isolation between the R and NR morphotypes of each group, and also taking into account geographic distances, using distance-based redundancy analysis (dbRDA). We also performed isolation by distance (IBD) tests between different floral morphotypes, aiming to infer the impact of genetic isolation on evolutionary and taxonomic divergence of the individuals. The taxa of Centaurea sects. Jacea and Lepteranthus constitute independent evolutionary lineages. Most of the detected genetic clusters match the taxonomic circumscription proposed in our most recent treatments. Genetic diversity was highest in C. debeauxii Godr. & Gren. subsp. debeauxii and subsp. grandiflora (Gaudin ex Schübl. & G. Martens) Devesa & Arnelas and in C. jacea L. subsp. angustifolia (DC.) Gremli (R) and lowest in C. nevadensis Boiss. & Reut. (NR) within the Centaurea sect. Jacea clade, whereas it was highest in C. linifolia L. within the Centaurea sect. Lepteranthus clade. Population-level dbRDA tests did not detect a significant correlation of R versus NR genetic distances and Fis values in any of the studied cases; by contrast, they detected significant correlation with longitude (C. jacea subsp. angustifolia, C. debeauxii, C. linifolia–C. stuessyi Arnelas, Devesa & E. López) or latitude (C. nigra L.). IBD analysis at the individual level showed that, in addition to geography, other morphotype-related factors may affect the genetic differentiation of R versus NR morphs in C. nigra, C. debeauxii, and C. linifolia–C. stuessyi. These results suggest that the fixation of the different morphs within the same taxon or group may be explained by geographic isolation, though we could not discount other potential unknown factors that could have contributed to microspeciation in these recently divergent populations, which originated only several thousands of years ago.

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来源期刊

Annals of the Missouri Botanical Garden 生物-植物科学

CiteScore

3.60

自引率

0.00%

发文量

期刊介绍： The Annals of the Missouri Botanical Garden is a quarterly international journal primarily devoted to systematic botany and evolutionary biology. We encourage submissions of original papers dealing with significant advances in the taxonomy, phylogeny, biogeography, paleobiology, and evolution of plants, and in conservation genetics and biology, restoration ecology, and ethnobiology, using morphological and/or molecular characters, field observations, and/or database information. We also welcome reviews and papers on conceptual issues and new methodologies in systematics. Important floristic works will also be considered. Symposium proceedings discussing a broader range of topical biological subjects are also published, typically once a year. All manuscripts are peer-reviewed by qualified and independent reviewers.