红花-阿利新蓝石蜡包埋植物切片的简单鉴别染色方法

IF 1.4 3区农林科学 Q2 FORESTRY

IAWA Journal Pub Date : 2023-04-21 DOI:10.1163/22941932-bja10124

Shunamit Wolberg, Mor Haim, Ilana Shtein

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引用次数: 2

摘要

本文提出了一种改进的石蜡包埋植物样品的藏红花-阿利新蓝染色方法。离开后，双重染色使用2:1的混合物，1%的藏红花和1%阿利新蓝。这种方法比以前发表的双重染色方案更便宜，因为它不需要绝对乙醇。此外，藏红花-阿利新蓝染色创造了一系列的色调，允许良好的细胞类型分化，它为各种各样的植物组织提供了可重复的结果，并且易于使用。我们希望它对研究和教学都有帮助。

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Simple differential staining method of paraffin-embedded plant sections with safranin-alcian blue

We present here a modified protocol for safranin-alcian blue staining of paraffin-embedded plant samples. After deparaffination, the double staining uses a 2:1 mix of 1% safranin and 1% alcian blue. This method is less expensive than previously published double staining protocols as it does not require absolute ethanol. Moreover, safranin-alcian blue staining creates a range of hues that allows good cell type differentiation, it gives reproducible results for a wide variety of plant tissues and is easy to use. We hope it will be found useful both for research and teaching purposes.

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来源期刊

IAWA Journal 农林科学-林学

CiteScore

3.40

自引率

15.80%

发文量

审稿时长

>36 weeks

期刊介绍： The IAWA Journal is the only international periodical fully devoted to structure, function, identification and utilisation of wood and bark in trees, shrubs, lianas, palms, bamboo and herbs. Many papers are of a multidisciplinary nature, linking