确定合适的三维生物打印支架结构在灌注生物反应器中培养:模拟和实验方法

IF 0.8 4区 医学 Q4 ENGINEERING, BIOMEDICAL
Jack Mankowsky, Connor Quigley, Scott Clark, A. Habib
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引用次数: 0

摘要

传统的细胞培养方法在为支架内的细胞提供生长介质的能力上受到限制。为了解决这个问题,我们开发了一种定制的灌注生物反应器,允许动态介质供应到包被或种子细胞。我们定制的生物反应器改善了体内刺激和条件,这可能会提高细胞的活力和增殖性能。其中一些努力包括使用双介质罐在不停止灌注的情况下取代介质,以及一个新设计的灌注室,可以容纳一系列磁带,允许各种各样的支架形状和大小。在本文中,我们利用模拟和实验方法探讨了流体流动对某些类型支架孔隙几何形状和孔隙度的响应。考虑了各种孔隙几何形状,如均匀三角形、正方形、菱形、圆形和具有均匀和可变尺寸的蜂窝。最后,对骨组织结构进行模拟和模拟,以确定流体流动的影响。在此基础上,确定了支架的最佳孔隙几何形状。我们探索了8%海藻酸盐、4%海藻酸盐-4%羧甲基纤维素(CMC)和2%海藻酸盐-6% CMC制备的支架的实时流体流动响应,允许流体在不同时间段(如1、2、4和8小时)恒定流动。通过膨胀率,即长丝宽度的变化和材料扩散,即培养前后干物质质量的比较来确定制备支架的变化。这项比较研究可以帮助基于应用的材料选择适合在灌注生物反应器中培养。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identifying Suitable Three-Dimensional Bio-Printed Scaffold Architectures to Incubate in a Perfusion Bioreactor: Simulation and Experimental Approaches
Traditional cell culturing methods are limited in their ability to supply growth medium to cells within scaffolds. To address this, we developed a custom perfusion bioreactor that allows for dynamic medium supply to encapsulated or seeded cells. Our custom-designed bioreactor improves the in vivo stimuli and conditions, which may enhance cell viability and proliferation performance. Some of the efforts include using dual medium tanks to replace the medium without stopping perfusion and a newly designed perfusion chamber that can accommodate an array of cassettes allowing for a wide assortment of scaffold shapes and sizes. In this paper, we explored the response of fluid flow to certain types of scaffold pore geometries and porosities using simulation and experimental approaches. Various pore geometries were considered, such as uniform triangular, square, diamond, circular, and honeycomb having uniform and variable sizes. Finally, bone tissue architecture was mimicked and simulated to identify the impact of fluid flow. Based on the results, optimum pore geometry for scaffolds were determined. We explored real-time fluid flow response on scaffolds fabricated with 8% Alginate, 4% Alginate-4% Carboxymethyl Cellulose (CMC), and 2% Alginate-6% CMC incubated, allowing a constant fluid flow for various periods such as 1, 2, 4, and 8 h. The change of fabricated scaffolds was determined in terms of swelling rate, i.e., change of filament width and material diffusion, i.e., comparison of dry material weight before and after incubation. This comparative study can assist in application-based materials selection suitable for incubating in a perfusion bioreactor.
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来源期刊
CiteScore
1.80
自引率
11.10%
发文量
56
审稿时长
6-12 weeks
期刊介绍: The Journal of Medical Devices presents papers on medical devices that improve diagnostic, interventional and therapeutic treatments focusing on applied research and the development of new medical devices or instrumentation. It provides special coverage of novel devices that allow new surgical strategies, new methods of drug delivery, or possible reductions in the complexity, cost, or adverse results of health care. The Design Innovation category features papers focusing on novel devices, including papers with limited clinical or engineering results. The Medical Device News section provides coverage of advances, trends, and events.
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