心肌长期细胞培养过程中1型和3A型DNA甲基转移酶转录物表达水平的增加

Q4 Biochemistry, Genetics and Molecular Biology

Medical Journal of Cell Biology Pub Date : 2021-03-01 DOI:10.2478/acb-2021-0005

M. Nawrocki, Rut Bryl, S. Kałużna, K. Stefańska, B. Stelmach, M. Jemielity, B. Perek, D. Bukowska, P. Mozdziak, J. Petitte, B. Kempisty

{"title":"心肌长期细胞培养过程中1型和3A型DNA甲基转移酶转录物表达水平的增加","authors":"M. Nawrocki, Rut Bryl, S. Kałużna, K. Stefańska, B. Stelmach, M. Jemielity, B. Perek, D. Bukowska, P. Mozdziak, J. Petitte, B. Kempisty","doi":"10.2478/acb-2021-0005","DOIUrl":null,"url":null,"abstract":"Abstract Heart failure (HF) is one of the main causes of death worldwide. Recent studies reported altered DNA methylation in failing human hearts. This may suggest a role of DNA methylation, most frequently implicated in epigenetic control, in the development of heart failure. Here, employing RT-qPCR, we characterized transcript levels for main DNA methyltransferases (DNMTs), DNMT1, DNMT3A, and DNMT3B, mediate DNA methylation, and they have different functions that complement each other during methylation. All analyzes were performed at different stages of porcine myocardial cell primary culture. In the present study we demonstrated increasing transcript expression levels for all analyzed genes during in vitro cultivation. The changes for DNMT1 and DNMT3A seem to be particularly important, where statistically significant changes were observed. Running title: DNMTs role in cardiac muscle cell culture","PeriodicalId":18329,"journal":{"name":"Medical Journal of Cell Biology","volume":"9 1","pages":"27 - 32"},"PeriodicalIF":0.0000,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Increased transcript expression levels of DNA methyltransferases type 1 and 3A during cardiac muscle long-term cell culture\",\"authors\":\"M. Nawrocki, Rut Bryl, S. Kałużna, K. Stefańska, B. Stelmach, M. Jemielity, B. Perek, D. Bukowska, P. Mozdziak, J. Petitte, B. Kempisty\",\"doi\":\"10.2478/acb-2021-0005\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract Heart failure (HF) is one of the main causes of death worldwide. Recent studies reported altered DNA methylation in failing human hearts. This may suggest a role of DNA methylation, most frequently implicated in epigenetic control, in the development of heart failure. Here, employing RT-qPCR, we characterized transcript levels for main DNA methyltransferases (DNMTs), DNMT1, DNMT3A, and DNMT3B, mediate DNA methylation, and they have different functions that complement each other during methylation. All analyzes were performed at different stages of porcine myocardial cell primary culture. In the present study we demonstrated increasing transcript expression levels for all analyzed genes during in vitro cultivation. The changes for DNMT1 and DNMT3A seem to be particularly important, where statistically significant changes were observed. Running title: DNMTs role in cardiac muscle cell culture\",\"PeriodicalId\":18329,\"journal\":{\"name\":\"Medical Journal of Cell Biology\",\"volume\":\"9 1\",\"pages\":\"27 - 32\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Medical Journal of Cell Biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2478/acb-2021-0005\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Medical Journal of Cell Biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2478/acb-2021-0005","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}

引用次数: 1

摘要

心衰(HF)是世界范围内导致死亡的主要原因之一。最近的研究报道了人类衰竭心脏中DNA甲基化的改变。这可能表明DNA甲基化的作用，最常见的涉及表观遗传控制，在心力衰竭的发展。本文采用RT-qPCR技术，研究了介导DNA甲基化的主要DNA甲基转移酶(dnmt) DNMT1、DNMT3A和DNMT3B的转录物水平，它们在甲基化过程中具有不同的功能，并相互补充。所有分析均在猪心肌细胞原代培养的不同阶段进行。在本研究中，我们证明了在体外培养过程中所有分析基因的转录表达水平增加。DNMT1和DNMT3A的变化似乎特别重要，在统计学上观察到显著的变化。题目:DNMTs在心肌细胞培养中的作用

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Increased transcript expression levels of DNA methyltransferases type 1 and 3A during cardiac muscle long-term cell culture

Abstract Heart failure (HF) is one of the main causes of death worldwide. Recent studies reported altered DNA methylation in failing human hearts. This may suggest a role of DNA methylation, most frequently implicated in epigenetic control, in the development of heart failure. Here, employing RT-qPCR, we characterized transcript levels for main DNA methyltransferases (DNMTs), DNMT1, DNMT3A, and DNMT3B, mediate DNA methylation, and they have different functions that complement each other during methylation. All analyzes were performed at different stages of porcine myocardial cell primary culture. In the present study we demonstrated increasing transcript expression levels for all analyzed genes during in vitro cultivation. The changes for DNMT1 and DNMT3A seem to be particularly important, where statistically significant changes were observed. Running title: DNMTs role in cardiac muscle cell culture

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Medical Journal of Cell Biology Biochemistry, Genetics and Molecular Biology-Cell Biology

自引率

0.00%

发文量