99Tcm-3PRGD2靶向受体显像对类风湿性关节炎血管生成的实验研究

中华核医学与分子影像杂志 Pub Date : 2020-03-25 DOI:10.3760/CMA.J.CN321828-20190923-00208

Yun-long Su

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引用次数: 0

摘要

目的探讨99Tcm肼基亚甲酰胺-（聚乙二醇）4-E[（聚乙二醇）-4-c（Arg-Gly-Asp）fK）]2（3PRGD2）在类风湿性关节炎（RA）早期诊断中的可行性。方法雌性Wistar大鼠60只，分为对照组（n=10，注射生理盐水0.3ml/只）和胶原诱导性关节炎（CIA）组（n=50，注射Ⅱ型胶原乳液0.3ml/支）。两组大鼠在建模前、建模后25天和45天接受99Tcm-3PRGD2平面成像。测量分析CIA大鼠模型建立前后病变关节和纵隔靶/非靶比值（T/NT）的变化，并与对照组大鼠进行比较。进行病理检查。采用重复测量方差分析和独立样本t检验对数据进行分析。结果CIA组成功建立32只大鼠滑膜，图像中可见明显的滑膜炎和滑膜增厚、新生血管形成。CIA组在造模前、造模后25和45d病变关节的T/NT分别为0.158±0.023、0.402±0.144、0.403±0.144，病变关节在造模后第25天和第45天的T/NT与对照组比较有显著性差异（0.160±0.028和0.158±0.032；T值分别为-10.484和-20.917，均P<0.01），αvβ3和肿瘤坏死因子-α表达。结论99Tcm-3PRGD2对大鼠类风湿性关节炎模型关节滑膜新生血管形成具有较高的敏感性，有望用于RA的早期诊断。关键词：关节炎、类风湿；新生血管，病理性；放射性核素成像；精氨酸-甘氨酸-天冬氨酸；大鼠

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Experimental study of 99Tcm-3PRGD2 targeted receptor imaging on angiogenesis in rheumatoid arthritis

Objective To investigate the feasibility of 99Tcm-hydrazinonicotinamide-(poly-(ethylene glycol))4-E[(poly-(ethylene glycol))4-c((Arg-Gly-Asp)fK)]2 (3PRGD2) in the early diagnosis of rheumatoid arthritis (RA). Methods Sixty female Wistar rats were divided into control group (n=10; injected with saline of 0.3 ml/piece) and collagen-induced arthritis (CIA) group (n=50; injected with type Ⅱ collagen emulsion of 0.3 ml/piece). Rats in 2 groups were subjected to 99Tcm-3PRGD2 planar imaging before modeling, 25 and 45 d after modeling. The changes of the target/non-target ratio (T/NT) of the lesion joint and mediastinum before and after modeling were measured and analyzed in CIA rats, and compared with rats in control group. Pathological examination was conducted. Repeated measures analysis of variance and independent-sample t test were used to analyze the data. Results Thirty-two rats in CIA group were successfully established, and obvious synovitis and synovial thickening, neovascularization were observed in the images. The T/NT of diseased joints in CIA group before modeling, 25 and 45 d after modeling were 0.158±0.023, 0.402±0.144, and 0.705±0.163 (F=286.924, P<0.01). The T/NT of diseased joints at 25 and 45 d after modeling were significantly different from those of control group (0.160±0.028 and 0.158±0.032; t values: -10.484 and -20.917, both P<0.01). Immunohistochemistry results showed positive expressions of vascular endothelial growth factor, αvβ3 and tumor necrosis factor-α in the synovial tissue in of diseased joints in rats of CIA group. Conclusion 99Tcm-3PRGD2 has high sensitivity for joint synovial neovascularization in rat rheumatoid arthritis models and is expected to be used for early diagnosis of RA. Key words: Arthritis, rheumatoid; Neovascularization, pathologic; Radionuclide imaging; Arginine-glycine-aspartic acid; Rats

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来源期刊

中华核医学与分子影像杂志核医学，分子影像

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5088

期刊介绍： Chinese Journal of Nuclear Medicine and Molecular Imaging (CJNMMI) was established in 1981, with the name of Chinese Journal of Nuclear Medicine, and renamed in 2012. As the specialized periodical in the domain of nuclear medicine in China, the aim of Chinese Journal of Nuclear Medicine and Molecular Imaging is to develop nuclear medicine sciences, push forward nuclear medicine education and basic construction, foster qualified personnel training and academic exchanges, and popularize related knowledge and raising public awareness. Topics of interest for Chinese Journal of Nuclear Medicine and Molecular Imaging include: -Research and commentary on nuclear medicine and molecular imaging with significant implications for disease diagnosis and treatment -Investigative studies of heart, brain imaging and tumor positioning -Perspectives and reviews on research topics that discuss the implications of findings from the basic science and clinical practice of nuclear medicine and molecular imaging - Nuclear medicine education and personnel training - Topics of interest for nuclear medicine and molecular imaging include subject coverage diseases such as cardiovascular diseases, cancer, Alzheimer’s disease, and Parkinson’s disease, and also radionuclide therapy, radiomics, molecular probes and related translational research.