Tiancheng Qiu, Harvey M. Andersen, Nissa J. Larson, N. Schroeder, J. Sweedler
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引用次数: 0
摘要
游离d -丝氨酸(D-Ser)是谷氨酸神经传递中n -甲基- d -天冬氨酸受体(NMDAR)的有效共激动剂,并调节NMDAR在神经系统中的功能。丝氨酸外消旋酶将l -丝氨酸转化为d -丝氨酸,被认为是动物体内d -丝氨酸的主要来源。在秀丽隐杆线虫中,丝氨酸消旋酶ser1的敲除会导致行为改变,但D-Ser的水平不受影响。通过在无蛋白胨线虫生长培养基(nf - ngm)上培养秀丽隐杆线虫,我们确定了D-Ser的来源,包括培养基中的外源蛋白胨和内源丝氨酸外消旋酶serr-1,以及一种潜在的丝氨酸/天冬氨酸外消旋酶Y51H7C。9、通过序列相似度网络分析进行识别。我们还在秀丽隐杆线虫中发现了一种新的丝氨酸脱水酶(又名丝氨酸解氨酶)K01C8.1。我们发现serr-1敲除和PF-NGM培养条件是影响秀丽隐杆线虫离食后运动行为的两个独立因素,无论是短期的还是长期的,两者之间没有相互作用。
Identifying sources of D-serine in Caenorhabditis elegans and their impact on behavior
Free D-serine (D-Ser) is a potent co-agonist of the N-methyl-D-aspartate receptor (NMDAR) in glutamate neurotransmission and regulates NMDAR functions in the nervous system. Serine racemases convert L-serine to D-Ser and are believed to be the major source of D-Ser in animals. In Caenorhabditis elegans, a knockout of the serine racemase serr-1 results in behavioral changes, but the level of D-Ser is unaffected. By growing C. elegans on peptone-free nematode growth medium (PF-NGM), we delineated the sources of D-Ser, both exogenous from peptone in culturing media and endogenous from the serine racemase serr-1, and a potential serine/aspartate racemase candidate, Y51H7C.9, identified by sequence similarity network analysis. We also discovered a new serine dehydratase (aka serine ammonia-lyase), K01C8.1, in C. elegans. We identified the serr-1 knockout and PF-NGM culturing conditions as two independent factors that impact C. elegans locomotion behavior after off-food, both short-term and long-term, and no interactions were found between the two factors.