{"title":"普通丸虫犰狳(armadillidium vulgare)外骨骼微生物群的初步研究","authors":"Sondos Alhajouj, D. Starkey","doi":"10.1894/0038-4909-67.1.10","DOIUrl":null,"url":null,"abstract":"Abstract The purpose of this study was to preliminarily examine the breadth of microbial diversity on the pill bug (Armadillidium vulgare) exoskeleton. To address this question, we collected pill bugs at four time points (approximately 3 months apart) and analyzed their external microbiotas. Preliminary results suggest that each time point contained a unique microbiome, suggesting a rapid turnover of the exoskeletal microbiota. In total, we recovered 15 unique isolates, which we resolved into three phyla and nine families. There were only three instances in which we recovered a single isolate from multiple time points, and we recovered only a single isolate from all four time points. Among the phyla identified, sequence divergence ranged from a minimum of 6.2% (among isolates recognized within the Actinobacteria) to a maximum of ∼34% (between isolates recognized within the Actinobacteria vs. b-proteobacteria). Overall, the results of this study suggest that in order to gain a complete understanding of the exoskeletal microbiota of pill bugs, and perhaps other arthropods, researchers must examine a temporal scale. Resumen El propósito de este estudio fue examinar preliminarmente la amplitud de la diversidad microbiana en el exoesqueleto de la cochinilla (Armadillidium vulgare). Para abordar esta pregunta, se recolectaron cochinillas en cuatro temporadas (aproximadamente tres meses entre si) y se analizaron sus microbiotas externas. Los resultados preliminares sugieren que cada temporada contuvo un microbioma único, lo que sugiere una rápida renovación de la microbiota exoesquelética. En total, se recuperaron 15 aislamientos únicos, que se resolvieron en tres filos y nueve familias. Solo hubo tres instancias en las que se recuperó un solo aislamiento de múltiples temporadas y se recuperó solo un aislamiento de todas las cuatro temporadas. Entre los filos identificados, la divergencia de secuencias varió desde un mínimo del 6.2% (entre aislados reconocidos dentro de Actinobacteria) hasta un máximo de ∼34% (entre aislados reconocidos dentro de Actinobacteria frente a b-proteobacteria). En general, los resultados de este estudio sugieren que para obtener una comprensión completa de la microbiota exoesquelética de las cochinillas y quizás de otros artrópodos, se debe examinar a escala temporal.","PeriodicalId":51157,"journal":{"name":"Southwestern Naturalist","volume":"67 1","pages":"10 - 17"},"PeriodicalIF":0.2000,"publicationDate":"2023-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"PRELIMINARY EXAMINATION OF THE EXOSKELETAL MICROBIOTA OF ARMADILLIDIUM VULGARE (THE COMMON PILL BUG)\",\"authors\":\"Sondos Alhajouj, D. Starkey\",\"doi\":\"10.1894/0038-4909-67.1.10\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract The purpose of this study was to preliminarily examine the breadth of microbial diversity on the pill bug (Armadillidium vulgare) exoskeleton. To address this question, we collected pill bugs at four time points (approximately 3 months apart) and analyzed their external microbiotas. Preliminary results suggest that each time point contained a unique microbiome, suggesting a rapid turnover of the exoskeletal microbiota. In total, we recovered 15 unique isolates, which we resolved into three phyla and nine families. There were only three instances in which we recovered a single isolate from multiple time points, and we recovered only a single isolate from all four time points. Among the phyla identified, sequence divergence ranged from a minimum of 6.2% (among isolates recognized within the Actinobacteria) to a maximum of ∼34% (between isolates recognized within the Actinobacteria vs. b-proteobacteria). Overall, the results of this study suggest that in order to gain a complete understanding of the exoskeletal microbiota of pill bugs, and perhaps other arthropods, researchers must examine a temporal scale. Resumen El propósito de este estudio fue examinar preliminarmente la amplitud de la diversidad microbiana en el exoesqueleto de la cochinilla (Armadillidium vulgare). Para abordar esta pregunta, se recolectaron cochinillas en cuatro temporadas (aproximadamente tres meses entre si) y se analizaron sus microbiotas externas. Los resultados preliminares sugieren que cada temporada contuvo un microbioma único, lo que sugiere una rápida renovación de la microbiota exoesquelética. En total, se recuperaron 15 aislamientos únicos, que se resolvieron en tres filos y nueve familias. Solo hubo tres instancias en las que se recuperó un solo aislamiento de múltiples temporadas y se recuperó solo un aislamiento de todas las cuatro temporadas. Entre los filos identificados, la divergencia de secuencias varió desde un mínimo del 6.2% (entre aislados reconocidos dentro de Actinobacteria) hasta un máximo de ∼34% (entre aislados reconocidos dentro de Actinobacteria frente a b-proteobacteria). En general, los resultados de este estudio sugieren que para obtener una comprensión completa de la microbiota exoesquelética de las cochinillas y quizás de otros artrópodos, se debe examinar a escala temporal.\",\"PeriodicalId\":51157,\"journal\":{\"name\":\"Southwestern Naturalist\",\"volume\":\"67 1\",\"pages\":\"10 - 17\"},\"PeriodicalIF\":0.2000,\"publicationDate\":\"2023-04-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Southwestern Naturalist\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://doi.org/10.1894/0038-4909-67.1.10\",\"RegionNum\":4,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Southwestern Naturalist","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1894/0038-4909-67.1.10","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
PRELIMINARY EXAMINATION OF THE EXOSKELETAL MICROBIOTA OF ARMADILLIDIUM VULGARE (THE COMMON PILL BUG)
Abstract The purpose of this study was to preliminarily examine the breadth of microbial diversity on the pill bug (Armadillidium vulgare) exoskeleton. To address this question, we collected pill bugs at four time points (approximately 3 months apart) and analyzed their external microbiotas. Preliminary results suggest that each time point contained a unique microbiome, suggesting a rapid turnover of the exoskeletal microbiota. In total, we recovered 15 unique isolates, which we resolved into three phyla and nine families. There were only three instances in which we recovered a single isolate from multiple time points, and we recovered only a single isolate from all four time points. Among the phyla identified, sequence divergence ranged from a minimum of 6.2% (among isolates recognized within the Actinobacteria) to a maximum of ∼34% (between isolates recognized within the Actinobacteria vs. b-proteobacteria). Overall, the results of this study suggest that in order to gain a complete understanding of the exoskeletal microbiota of pill bugs, and perhaps other arthropods, researchers must examine a temporal scale. Resumen El propósito de este estudio fue examinar preliminarmente la amplitud de la diversidad microbiana en el exoesqueleto de la cochinilla (Armadillidium vulgare). Para abordar esta pregunta, se recolectaron cochinillas en cuatro temporadas (aproximadamente tres meses entre si) y se analizaron sus microbiotas externas. Los resultados preliminares sugieren que cada temporada contuvo un microbioma único, lo que sugiere una rápida renovación de la microbiota exoesquelética. En total, se recuperaron 15 aislamientos únicos, que se resolvieron en tres filos y nueve familias. Solo hubo tres instancias en las que se recuperó un solo aislamiento de múltiples temporadas y se recuperó solo un aislamiento de todas las cuatro temporadas. Entre los filos identificados, la divergencia de secuencias varió desde un mínimo del 6.2% (entre aislados reconocidos dentro de Actinobacteria) hasta un máximo de ∼34% (entre aislados reconocidos dentro de Actinobacteria frente a b-proteobacteria). En general, los resultados de este estudio sugieren que para obtener una comprensión completa de la microbiota exoesquelética de las cochinillas y quizás de otros artrópodos, se debe examinar a escala temporal.
期刊介绍:
The Southwestern Naturalist (a publication of the Southwestern Association of Naturalists since 1953) is an international journal (published quarterly) that reports original and significant research in any field of natural history. This journal promotes the study of plants and animals (living and fossil) in the multinational region that includes the southwestern United States, Mexico, and Central America. Appropriate submission of manuscripts may come from studies conducted in the countries of focus or in regions outside this area that report significant findings relating to biota occurring in the southwestern United States, Mexico, and Central America. Publication is in English, and manuscripts may be feature articles or notes. Feature articles communicate results of completed scientific investigations, while notes are reserved for short communications (e.g., behavioral observations, range extensions, and other important findings that do not in themselves constitute a comprehensive study). All manuscripts (feature articles and notes) require an abstract in both English and Spanish.