一种新的生物反应器,可在有或无激活素A的情况下促进变性男性卵泡生长

IF 1.1 Q4 OBSTETRICS & GYNECOLOGY
C. Jovet, E. Fraison, J. Lornage, N. M. Journel, A. Gavoille, L. David, A. Montembault, C. Fournier, B. Salle, E. Labrune
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引用次数: 0

摘要

本研究的目的是评估激活素A在具有或不具有我们新的能够实现三维组织培养的分隔壳聚糖水凝胶微生物反应器(“三维(3D)结构”)的情况下对转基因男性人类卵巢组织体外卵泡发生的激活作用。在培养的最后六到八天中,将五个新鲜的卵巢人类组织分为四组,在体外培养20或22天,在标准条件下,用100ng/mL激活素A或不用激活素A。评估卵泡密度和质量,测量卵泡直径。对雌二醇分泌进行定量。还通过免疫染色进行增殖和凋亡。四组原始卵泡的比例均显著降低,初级卵泡和次级卵泡的比例显著增加(p<0.001)。四个培养组均观察到第三代卵泡。激活素A补充对卵泡密度、卵泡质量、卵泡生长没有显著影响,或雌二醇分泌(p>0.05)。3D结构增加了原代卵泡的密度,降低了雌二醇的分泌(p<0.001)。与非3D结构组相比,3D结构组的卵泡增殖显著降低(p=0.008)。关于卵泡凋亡,激活素组明显高于非激活素对照组(p=0.006)。在我们的培养条件下,激活素A似乎在体外卵泡生成激活中没有发挥关键作用。然而,研究结果可能表明,3D结构可能更具生理性,并可以防止有害的体外卵泡生成爆发。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A New Bioreactor to Promote Human Follicular Growth with or without Activin A in Transgender Men
The aim of the present study was to evaluate the effect of activin A on the activation of in vitro folliculogenesis of human ovarian tissues from transgender men with or without our new compartmented chitosan hydrogel microbioreactor (“three-dimensional (3D)-structure”) enabling a three-dimensional tissue culture. Five fresh ovarian human tissues were cultured in vitro for 20 or 22 days in four groups with 100 ng/mL activin A or without activin A during the last six to eight days of culture, and within a 3D-structure or without the 3D-structure in standard conditions. Follicular density and quality were evaluated, and follicular diameters were measured. Estradiol secretion was quantified. Proliferation and apoptosis through immunostaining were also performed. The proportion of primordial follicles was significantly reduced, and the proportion of primary and secondary follicles was significantly increased in all four groups (p < 0.001). Tertiary follicles were observed in the four culture groups. Activin A supplementation did not significantly affect the follicular density, follicular quality, follicular growth, or estradiol secretion (p > 0.05). The 3D-structure increased the density of primary follicles and decreased the estradiol secretion (p < 0.001). Follicular proliferation was significantly lower in the 3D-structure group compared to the non-3D-structure group (p = 0.008). Regarding follicular apoptosis, it was significantly higher in the activin group compared to the non-activin group (p = 0.006). Activin A did not seem to play a key role in the in vitro folliculogenesis activation in our culture conditions. However, the results may indicate that the 3D-structure could be more physiological and could prevent a detrimental in vitro folliculogenesis flare-up.
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