血管生成素(AGN)KO小鼠与肌肉力量、抵抗力改善但肿瘤发生几率降低之间缺乏相关性:观察研究

M. Malone, Erika Mejia Hidalgo, Guofu Hu
{"title":"血管生成素(AGN)KO小鼠与肌肉力量、抵抗力改善但肿瘤发生几率降低之间缺乏相关性:观察研究","authors":"M. Malone, Erika Mejia Hidalgo, Guofu Hu","doi":"10.4172/2324-9110.1000172","DOIUrl":null,"url":null,"abstract":"Background: For quite a while, there has been controversy over whether or not angiogenin is associated with amyotrophic lateral sclerosis (ALS) risk. A lot of meta-analysis has been done in the last few years to support the fact that angiogenin has no relationship in improving the outcome and muscle strength in ALS. In the other hand, for about 3 decades angiogenin has been thought to play an important role in cell survival, growth, and proliferation. Evidence suggests that the expression and activity of ANG increases the odds of developing a variety of human cancers. We present the results of an observational study done in wild type (WT) and angiogenin knock out (ANG -/- KO) mice in order to determine phenotype and different features as explained below. \nMethods: We used 2 population of mice, 5 WT and 5 ANG -/- KO were involved in the study. They all were old, each 12 weeks old. These 2 populations were studied for a total of 8 weeks. We invested about 4 hours per day, 5 out 7 days per week. We had the mice running at different speeds on a treadmill in cycles of 300 seconds each, repeating these between 7-10 cycles per population. We used different speeds, being anywhere between 1 MPH to 7 MPH at the highest. We tested our theory that mice ANG -/- KO will have better strength and resistance by observing whether the mice looked stronger compared to the other population. Also by observation, we looked for physical abnormalities as well as evident malignancies in the ANG -/- KO vs. WT mice. \nResults: After conducting this study for 8 weeks, we found that ANG -/- KO mice did not have any statistical significant (p=0.36) muscle strength or resistance improvement compared to the WT mice. Also, at the end of our study we could conclude that ANG -/- KO did have less alopecia, skin cancer (basal cell carcinomas; squamous cell carcinomas), benign lesions of the skin, and malignant development of lymph nodes compared to WT mice. All 5 mice in the WT population did have skin lesions consistent with skin cancer and lymphadenopathy consistent with malignant lymph nodes of either lymphomas or metastatic disease. \nDiscussion: At the end of the study, we could conclude that there was no statistical significance while evaluating both WT and KO mice as they had equal muscle strength and resistance. Conversely, we did see an increase in the number of benign and malignant proliferations in WT mice vs. KO mice.","PeriodicalId":73658,"journal":{"name":"Journal of clinical & experimental oncology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2017-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":"{\"title\":\"Lack of Association between Angiogenin (AGN) KO Mice with Improvement in Muscle Strength, Resistance,but Decreased in Chances of Tumorigenesis: Observational Study\",\"authors\":\"M. Malone, Erika Mejia Hidalgo, Guofu Hu\",\"doi\":\"10.4172/2324-9110.1000172\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background: For quite a while, there has been controversy over whether or not angiogenin is associated with amyotrophic lateral sclerosis (ALS) risk. A lot of meta-analysis has been done in the last few years to support the fact that angiogenin has no relationship in improving the outcome and muscle strength in ALS. In the other hand, for about 3 decades angiogenin has been thought to play an important role in cell survival, growth, and proliferation. Evidence suggests that the expression and activity of ANG increases the odds of developing a variety of human cancers. We present the results of an observational study done in wild type (WT) and angiogenin knock out (ANG -/- KO) mice in order to determine phenotype and different features as explained below. \\nMethods: We used 2 population of mice, 5 WT and 5 ANG -/- KO were involved in the study. They all were old, each 12 weeks old. These 2 populations were studied for a total of 8 weeks. We invested about 4 hours per day, 5 out 7 days per week. We had the mice running at different speeds on a treadmill in cycles of 300 seconds each, repeating these between 7-10 cycles per population. We used different speeds, being anywhere between 1 MPH to 7 MPH at the highest. We tested our theory that mice ANG -/- KO will have better strength and resistance by observing whether the mice looked stronger compared to the other population. Also by observation, we looked for physical abnormalities as well as evident malignancies in the ANG -/- KO vs. WT mice. \\nResults: After conducting this study for 8 weeks, we found that ANG -/- KO mice did not have any statistical significant (p=0.36) muscle strength or resistance improvement compared to the WT mice. Also, at the end of our study we could conclude that ANG -/- KO did have less alopecia, skin cancer (basal cell carcinomas; squamous cell carcinomas), benign lesions of the skin, and malignant development of lymph nodes compared to WT mice. All 5 mice in the WT population did have skin lesions consistent with skin cancer and lymphadenopathy consistent with malignant lymph nodes of either lymphomas or metastatic disease. \\nDiscussion: At the end of the study, we could conclude that there was no statistical significance while evaluating both WT and KO mice as they had equal muscle strength and resistance. Conversely, we did see an increase in the number of benign and malignant proliferations in WT mice vs. KO mice.\",\"PeriodicalId\":73658,\"journal\":{\"name\":\"Journal of clinical & experimental oncology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2017-02-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of clinical & experimental oncology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4172/2324-9110.1000172\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of clinical & experimental oncology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4172/2324-9110.1000172","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 4

摘要

背景:很长一段时间以来,血管生成素是否与肌萎缩侧索硬化(ALS)风险相关一直存在争议。在过去的几年里,很多荟萃分析都支持血管生成素在改善ALS的结果和肌肉力量方面没有任何关系。另一方面,近30年来,血管生成素一直被认为在细胞存活、生长和增殖中起着重要作用。有证据表明,ANG的表达和活性增加了患多种人类癌症的几率。我们在野生型(WT)和血管生成素敲除(ANG -/- KO)小鼠中进行了一项观察性研究,以确定表型和不同的特征,如下所述。方法:采用2群小鼠,WT 5只,ANG -/- KO 5只。它们都很老,都是12周大。对这两个种群进行了为期8周的研究。我们每天投入4个小时,每周投入5到7天。我们让老鼠在跑步机上以不同的速度跑步,每组300秒,每组重复7-10次。我们使用了不同的速度,最高时速在1英里到7英里之间。我们通过观察小鼠是否看起来比其他种群更强壮来验证我们的理论,即小鼠ANG -/- KO将具有更好的力量和抵抗力。同样通过观察,我们在ANG -/- KO与WT小鼠中寻找身体异常以及明显的恶性肿瘤。结果:经过8周的研究,我们发现ANG -/- KO小鼠与WT小鼠相比,肌肉力量和阻力的改善没有统计学意义(p=0.36)。此外,在我们的研究结束时,我们可以得出结论,ANG -/- KO确实有较少的脱发,皮肤癌(基底细胞癌;鳞状细胞癌),皮肤良性病变和淋巴结恶性发展与WT小鼠相比。WT群体中的所有5只小鼠都有与皮肤癌一致的皮肤损伤和与淋巴瘤或转移性疾病的恶性淋巴结一致的淋巴结病。讨论:在研究结束时,我们可以得出结论,WT和KO小鼠在评估时没有统计学意义,因为它们具有相同的肌肉力量和阻力。相反,我们确实看到WT小鼠与KO小鼠的良性和恶性增殖数量增加。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Lack of Association between Angiogenin (AGN) KO Mice with Improvement in Muscle Strength, Resistance,but Decreased in Chances of Tumorigenesis: Observational Study
Background: For quite a while, there has been controversy over whether or not angiogenin is associated with amyotrophic lateral sclerosis (ALS) risk. A lot of meta-analysis has been done in the last few years to support the fact that angiogenin has no relationship in improving the outcome and muscle strength in ALS. In the other hand, for about 3 decades angiogenin has been thought to play an important role in cell survival, growth, and proliferation. Evidence suggests that the expression and activity of ANG increases the odds of developing a variety of human cancers. We present the results of an observational study done in wild type (WT) and angiogenin knock out (ANG -/- KO) mice in order to determine phenotype and different features as explained below. Methods: We used 2 population of mice, 5 WT and 5 ANG -/- KO were involved in the study. They all were old, each 12 weeks old. These 2 populations were studied for a total of 8 weeks. We invested about 4 hours per day, 5 out 7 days per week. We had the mice running at different speeds on a treadmill in cycles of 300 seconds each, repeating these between 7-10 cycles per population. We used different speeds, being anywhere between 1 MPH to 7 MPH at the highest. We tested our theory that mice ANG -/- KO will have better strength and resistance by observing whether the mice looked stronger compared to the other population. Also by observation, we looked for physical abnormalities as well as evident malignancies in the ANG -/- KO vs. WT mice. Results: After conducting this study for 8 weeks, we found that ANG -/- KO mice did not have any statistical significant (p=0.36) muscle strength or resistance improvement compared to the WT mice. Also, at the end of our study we could conclude that ANG -/- KO did have less alopecia, skin cancer (basal cell carcinomas; squamous cell carcinomas), benign lesions of the skin, and malignant development of lymph nodes compared to WT mice. All 5 mice in the WT population did have skin lesions consistent with skin cancer and lymphadenopathy consistent with malignant lymph nodes of either lymphomas or metastatic disease. Discussion: At the end of the study, we could conclude that there was no statistical significance while evaluating both WT and KO mice as they had equal muscle strength and resistance. Conversely, we did see an increase in the number of benign and malignant proliferations in WT mice vs. KO mice.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信